Functional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72578
Title:
Functional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro.
Authors:
Wang, Qiuyu; Kumar, Shant; Slevin, Mark; Kumar, Patricia
Abstract:
Transcription factor PAX3 has seven isoforms of which PAX3c has been studied extensively whereas the functions of the other isoforms are less well known. Here, we found that PAX3 isoforms in a stable transfection system have different biological functions in mouse melanocytes in vitro. PAX3a and PAX3b had negative effects on melanocyte proliferation but had no discernable effect on melanocyte growth in soft agar. PAX3a did not affect cell migration and apoptosis but PAX3b reduced migration and accelerated apoptosis. PAX3c and PAX3d promoted cell proliferation, migration, transformation, and survival. PAX3e reduced melanocyte growth; transformation and migration were unchanged and apoptosis was increased in vitro. PAX3g did not influence cell proliferation or apoptosis. Cells expressing PAX3g were able to grow in soft agar but migration was reduced. PAX3h increased cell proliferation, migration, survival, and transformation. These functional studies have advanced our understanding of the effects of PAX3 isoforms in melanocytes and their potential contribution in tumorigenesis.
Affiliation:
School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.
Citation:
Functional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro. 2006, 66 (17):8574-80 Cancer Res.
Journal:
Cancer Research
Issue Date:
1-Sep-2006
URI:
http://hdl.handle.net/10541/72578
DOI:
10.1158/0008-5472.CAN-06-0947
PubMed ID:
16951170
Type:
Article
Language:
en
ISSN:
1538-7445
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Qiuyu-
dc.contributor.authorKumar, Shant-
dc.contributor.authorSlevin, Mark-
dc.contributor.authorKumar, Patricia-
dc.date.accessioned2009-07-06T11:46:16Z-
dc.date.available2009-07-06T11:46:16Z-
dc.date.issued2006-09-01-
dc.identifier.citationFunctional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro. 2006, 66 (17):8574-80 Cancer Res.en
dc.identifier.issn1538-7445-
dc.identifier.pmid16951170-
dc.identifier.doi10.1158/0008-5472.CAN-06-0947-
dc.identifier.urihttp://hdl.handle.net/10541/72578-
dc.description.abstractTranscription factor PAX3 has seven isoforms of which PAX3c has been studied extensively whereas the functions of the other isoforms are less well known. Here, we found that PAX3 isoforms in a stable transfection system have different biological functions in mouse melanocytes in vitro. PAX3a and PAX3b had negative effects on melanocyte proliferation but had no discernable effect on melanocyte growth in soft agar. PAX3a did not affect cell migration and apoptosis but PAX3b reduced migration and accelerated apoptosis. PAX3c and PAX3d promoted cell proliferation, migration, transformation, and survival. PAX3e reduced melanocyte growth; transformation and migration were unchanged and apoptosis was increased in vitro. PAX3g did not influence cell proliferation or apoptosis. Cells expressing PAX3g were able to grow in soft agar but migration was reduced. PAX3h increased cell proliferation, migration, survival, and transformation. These functional studies have advanced our understanding of the effects of PAX3 isoforms in melanocytes and their potential contribution in tumorigenesis.en
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectCancer RNAen
dc.subject.meshCell Cycle-
dc.subject.meshCell Division-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Movement-
dc.subject.meshEmbryonic Development-
dc.subject.meshHumans-
dc.subject.meshMelanocytes-
dc.subject.meshMelanoma-
dc.subject.meshNeural Crest-
dc.subject.meshPaired Box Transcription Factors-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshProtein Isoforms-
dc.subject.meshRNA, Neoplasm-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction-
dc.titleFunctional analysis of alternative isoforms of the transcription factor PAX3 in melanocytes in vitro.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.en
dc.identifier.journalCancer Researchen

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