A comparative study of neovascularisation in atherosclerotic plaques using CD31, CD105 and TGF beta 1.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72433
Title:
A comparative study of neovascularisation in atherosclerotic plaques using CD31, CD105 and TGF beta 1.
Authors:
Li, Chenggang; Mollahan, Pamela; Baguneid, Mohamed S; McMahon, R; Kumar, Patricia; Walker, Michael G; Freemont, Anthony J; Kumar, Shant
Abstract:
OBJECTIVES: This study aims to identify plaque neovascularisation using antibodies to CD31, CD105 and TGFbeta1, and to compare their patterns of expression. METHODS: Tissue expression of CD31, CD105 and TGFbeta1 was examined immunohistologically in atherosclerotic plaques from 53 patients who had undergone carotid endarterectomy and in 10 controls. RESULTS: CD31 was observed in a proportion of the microvessels within atheroma. The expression of CD105 was barely visible in normal arteries, but was markedly enhanced in atherosclerotic plaques. The vast majority of the microvessels in atheroma were positive for CD105 with pronounced expression around the periphery of the lipid core. In consecutive sections, microvessels showing negative staining for CD31 were positive for CD105. Although TGFbeta1 was seen in the thickened intima, it was more strongly expressed in well-formed fibrous plaques. Consecutive sections showed that some microvessels were stained by both CD105 and TGFbeta1, but in certain areas microvessels were exclusively CD105 positive. CONCLUSIONS: These observations highlight the distinctive expression patterns of CD31, CD105 and TGFbeta1, suggesting their specific roles in the development of atherosclerotic plaques. CD105 is almost universally expressed in microvessels within the atheroma and is therefore a better vascular marker than CD31 and TGFbeta1for assessing neovascularisation in atherosclerotic plaques.
Affiliation:
Department of Pathology, Medical School and Christie Hospital, University of Manchester, Manchester, UK.
Citation:
A comparative study of neovascularisation in atherosclerotic plaques using CD31, CD105 and TGF beta 1. 2006, 73 (4):192-7 Pathobiology
Journal:
Pathobiology
Issue Date:
2006
URI:
http://hdl.handle.net/10541/72433
DOI:
10.1159/000096020
PubMed ID:
17119348
Type:
Article
Language:
en
ISSN:
1015-2008
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Chenggang-
dc.contributor.authorMollahan, Pamela-
dc.contributor.authorBaguneid, Mohamed S-
dc.contributor.authorMcMahon, R-
dc.contributor.authorKumar, Patricia-
dc.contributor.authorWalker, Michael G-
dc.contributor.authorFreemont, Anthony J-
dc.contributor.authorKumar, Shant-
dc.date.accessioned2009-07-03T11:33:40Z-
dc.date.available2009-07-03T11:33:40Z-
dc.date.issued2006-
dc.identifier.citationA comparative study of neovascularisation in atherosclerotic plaques using CD31, CD105 and TGF beta 1. 2006, 73 (4):192-7 Pathobiologyen
dc.identifier.issn1015-2008-
dc.identifier.pmid17119348-
dc.identifier.doi10.1159/000096020-
dc.identifier.urihttp://hdl.handle.net/10541/72433-
dc.description.abstractOBJECTIVES: This study aims to identify plaque neovascularisation using antibodies to CD31, CD105 and TGFbeta1, and to compare their patterns of expression. METHODS: Tissue expression of CD31, CD105 and TGFbeta1 was examined immunohistologically in atherosclerotic plaques from 53 patients who had undergone carotid endarterectomy and in 10 controls. RESULTS: CD31 was observed in a proportion of the microvessels within atheroma. The expression of CD105 was barely visible in normal arteries, but was markedly enhanced in atherosclerotic plaques. The vast majority of the microvessels in atheroma were positive for CD105 with pronounced expression around the periphery of the lipid core. In consecutive sections, microvessels showing negative staining for CD31 were positive for CD105. Although TGFbeta1 was seen in the thickened intima, it was more strongly expressed in well-formed fibrous plaques. Consecutive sections showed that some microvessels were stained by both CD105 and TGFbeta1, but in certain areas microvessels were exclusively CD105 positive. CONCLUSIONS: These observations highlight the distinctive expression patterns of CD31, CD105 and TGFbeta1, suggesting their specific roles in the development of atherosclerotic plaques. CD105 is almost universally expressed in microvessels within the atheroma and is therefore a better vascular marker than CD31 and TGFbeta1for assessing neovascularisation in atherosclerotic plaques.en
dc.language.isoenen
dc.subject.meshAntigens, CD-
dc.subject.meshAntigens, CD31-
dc.subject.meshAtherosclerosis-
dc.subject.meshBiological Markers-
dc.subject.meshCarotid Stenosis-
dc.subject.meshHumans-
dc.subject.meshImmunoenzyme Techniques-
dc.subject.meshNeovascularization, Pathologic-
dc.subject.meshReceptors, Cell Surface-
dc.subject.meshTransforming Growth Factor beta1-
dc.titleA comparative study of neovascularisation in atherosclerotic plaques using CD31, CD105 and TGF beta 1.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, Medical School and Christie Hospital, University of Manchester, Manchester, UK.en
dc.identifier.journalPathobiologyen

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