The fate of human Langerhans cells in hematopoietic stem cell transplantation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/72416
Title:
The fate of human Langerhans cells in hematopoietic stem cell transplantation.
Authors:
Collin, Matthew P; Hart, Derek N J; Jackson, Graham H; Cook, Gordon; Cavet, James; Mackinnon, Stephen; Middleton, Peter G; Dickinson, Anne M
Abstract:
Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14-21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.
Affiliation:
Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK. matthew.collin@ncl.ac.uk
Citation:
The fate of human Langerhans cells in hematopoietic stem cell transplantation. 2006, 203 (1):27-33 J. Exp. Med.
Journal:
The Journal of Experimental Medicine
Issue Date:
23-Jan-2006
URI:
http://hdl.handle.net/10541/72416
DOI:
10.1084/jem.20051787
PubMed ID:
16390938
Type:
Article
Language:
en
ISSN:
0022-1007
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorCollin, Matthew P-
dc.contributor.authorHart, Derek N J-
dc.contributor.authorJackson, Graham H-
dc.contributor.authorCook, Gordon-
dc.contributor.authorCavet, James-
dc.contributor.authorMackinnon, Stephen-
dc.contributor.authorMiddleton, Peter G-
dc.contributor.authorDickinson, Anne M-
dc.date.accessioned2009-07-03T12:05:52Z-
dc.date.available2009-07-03T12:05:52Z-
dc.date.issued2006-01-23-
dc.identifier.citationThe fate of human Langerhans cells in hematopoietic stem cell transplantation. 2006, 203 (1):27-33 J. Exp. Med.en
dc.identifier.issn0022-1007-
dc.identifier.pmid16390938-
dc.identifier.doi10.1084/jem.20051787-
dc.identifier.urihttp://hdl.handle.net/10541/72416-
dc.description.abstractLangerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14-21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cell Transplantationen
dc.subject.meshAdult-
dc.subject.meshFemale-
dc.subject.meshGraft vs Host Disease-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshHumans-
dc.subject.meshLangerhans Cells-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshTransplantation Chimera-
dc.subject.meshTransplantation Conditioning-
dc.titleThe fate of human Langerhans cells in hematopoietic stem cell transplantation.en
dc.typeArticleen
dc.contributor.departmentHaematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK. matthew.collin@ncl.ac.uken
dc.identifier.journalThe Journal of Experimental Medicineen

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