The fate of human Langerhans cells in hematopoietic stem cell transplantation.
Authors
Collin, Matthew PHart, Derek N J
Jackson, Graham H
Cook, Gordon
Cavet, James
Mackinnon, Stephen
Middleton, Peter G
Dickinson, Anne M
Affiliation
Haematological Sciences, University of Newcastle, Newcastle upon Tyne NE2 4HH, England, UK. matthew.collin@ncl.ac.ukIssue Date
2006-01-23
Metadata
Show full item recordAbstract
Langerhans cells (LC) and other antigen-presenting cells are believed to be critical in initiating graft versus host responses that influence the outcome of allogeneic hematopoietic stem cell transplantation. However, their fate in humans is poorly understood. We have sought to define the effect of conditioning regimes and graft versus host disease (GVHD) on the survival of recipient LC and reconstitution of donor cells after transplant. Confocal microscopy of epidermal sheets shows that full intensity transplant (FIT) depletes LC more rapidly than reduced intensity transplant (RIT) at day 0, although the nadir is similar in both at 14-21 d. Recovery occurs rapidly within 40 d in the absence of acute GVHD, but is delayed beyond 100 d when GVHD is active. LC chimerism was determined in sex-mismatched transplants using a two-step Giemsa/fluorescence in situ hybridization assay on isolated cells. Acquisition of donor chimerism at 40 d is more rapid after FIT (97%) than RIT (36.5%), irrespective of blood myeloid engraftment. At 100 d, all transplants achieve at least 90% LC donor chimerism and over half achieve 100%. Complete donor chimerism is associated with prior acute cutaneous GVHD, suggesting a role for allogeneic T cells in promoting LC engraftment.Citation
The fate of human Langerhans cells in hematopoietic stem cell transplantation. 2006, 203 (1):27-33 J. Exp. Med.Journal
The Journal of Experimental MedicineDOI
10.1084/jem.20051787PubMed ID
16390938Type
ArticleLanguage
enISSN
0022-1007ae974a485f413a2113503eed53cd6c53
10.1084/jem.20051787