PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.

2.50
Hdl Handle:
http://hdl.handle.net/10541/71956
Title:
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.
Authors:
Li, Hong Gui; Wang, Qiuyu; Hong, Mei Li; Kumar, Shant; Parker, Craig; Slevin, Mark; Kumar, Patricia
Abstract:
PAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.
Affiliation:
School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom.
Citation:
PAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN. 2007, 253 (2):215-23 Cancer Lett.
Journal:
Cancer Letters
Issue Date:
18-Aug-2007
URI:
http://hdl.handle.net/10541/71956
DOI:
10.1016/j.canlet.2007.01.020
PubMed ID:
17350164
Type:
Article
Language:
en
ISSN:
0304-3835
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorLi, Hong Gui-
dc.contributor.authorWang, Qiuyu-
dc.contributor.authorHong, Mei Li-
dc.contributor.authorKumar, Shant-
dc.contributor.authorParker, Craig-
dc.contributor.authorSlevin, Mark-
dc.contributor.authorKumar, Patricia-
dc.date.accessioned2009-06-30T14:08:21Z-
dc.date.available2009-06-30T14:08:21Z-
dc.date.issued2007-08-18-
dc.identifier.citationPAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN. 2007, 253 (2):215-23 Cancer Lett.en
dc.identifier.issn0304-3835-
dc.identifier.pmid17350164-
dc.identifier.doi10.1016/j.canlet.2007.01.020-
dc.identifier.urihttp://hdl.handle.net/10541/71956-
dc.description.abstractPAX3 or PAX3-FKHR expression is implicated in cell transformation and tumourigenesis. Here, C2C12 myoblasts were transfected with a sense Pax3 vector and a pTet-On system to induce Pax3 expression, whereas to downregulate PAX3-FKHR, Rh18 was transfected with an antisense Pax3 with a pTet-On system. The inhibition of PAX3-FKHR in Rh18 induced upregulation of PTEN. Decreased resistance to apoptosis and increased transformation ability were observed in the Rh18 cells with PAX3-FKHR downregulation. Conversely, Pax3 induction in C2C12 cells downregulated the expression of PTEN and p27(Kip1). These results indicate that the involvement of PAX3 and PAX3-FKHR in rhabdomyosarcoma tumourigenesis may be through downregulation of PTEN tumour suppressor gene, affecting the PTEN/AKT survival pathway.en
dc.language.isoenen
dc.subjectCell Line Tumouren
dc.subjectMuscle Canceren
dc.subject.meshAnimals-
dc.subject.meshCell Line, Tumor-
dc.subject.meshCell Survival-
dc.subject.meshCell Transformation, Neoplastic-
dc.subject.meshChromatin Immunoprecipitation-
dc.subject.meshCyclin-Dependent Kinase Inhibitor p27-
dc.subject.meshDown-Regulation-
dc.subject.meshDoxycycline-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshNeoplasms, Muscle Tissue-
dc.subject.meshOncogene Proteins, Fusion-
dc.subject.meshPTEN Phosphohydrolase-
dc.subject.meshPaired Box Transcription Factors-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshRhabdomyosarcoma-
dc.titlePAX3 and PAX3-FKHR promote rhabdomyosarcoma cell survival through downregulation of PTEN.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester M1 5GD, United Kingdom.en
dc.identifier.journalCancer Lettersen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.