Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy.

2.50
Hdl Handle:
http://hdl.handle.net/10541/71914
Title:
Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy.
Authors:
Kiesslich, Ralf; Goetz, Martin; Angus, Elizabeth M; Hu, Qiuping; Guan, Yanfang; Potten, Christopher S; Allen, Terence D; Neurath, Markus F; Shroyer, Noah F; Montrose, Marshall H; Watson, Alastair
Abstract:
BACKGROUND & AIMS: Confocal endomicroscopy is an emerging technology that poses the endoscopist with challenges for identifying epithelial structures in the human intestine. We have shown previously that the murine intestinal epithelium is punctuated by gaps caused by cell shedding. The goals of this study were to determine if confocal endomicroscopy could resolve the presence of human epithelial gaps and whether a proinflammatory cytokine could increase cell shedding. METHODS: Intestinal mucosa was imaged after staining with acriflavine. Confocal endomicroscopy of 17 patients yielded 6277 images from the human terminal ileum and rectum. Results were validated by parallel studies of anesthetized mice (wild-type and Math1(DeltaIntestine)) using rigid confocal probe microscopy, 2-photon/confocal microscopy, and scanning electron microscopy. RESULTS: Human terminal ileal and rectal epithelium revealed unstained areas with the diameter of an individual epithelial cell, with 2 distinct morphologies. One had a "target" appearance, shown by mouse studies to be goblet cells. The other morphology had no nucleus and was observed by rigid confocal probe microscopy and scanning electron microscopy in the villi of Math1(DeltaIntestine) mice, which lack goblet cells. In the mouse, tumor necrosis factor alpha (0.33 microg/g intraperitoneally) increases cell shedding by 27-fold and caused loss of barrier function across 20% of resultant gaps. CONCLUSIONS: Confocal endomicroscopy can distinguish between epithelial discontinuities (gaps) and goblet cells in human intestine. Results suggest that the sealing of epithelial gaps must be considered as a component of the intestinal barrier and has potential implications for intestinal barrier dysfunction in human disease.
Affiliation:
I Medical Clinic, University of Mainz, Mainz, Germany.
Citation:
Identification of epithelial gaps in human small and large intestine by confocal endomicroscopy. 2007, 133 (6):1769-78 Gastroenterology
Journal:
Gastroenterology
Issue Date:
Dec-2007
URI:
http://hdl.handle.net/10541/71914
DOI:
10.1053/j.gastro.2007.09.011
PubMed ID:
18054549
Type:
Article
Language:
en
ISSN:
1528-0012
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorKiesslich, Ralf-
dc.contributor.authorGoetz, Martin-
dc.contributor.authorAngus, Elizabeth M-
dc.contributor.authorHu, Qiuping-
dc.contributor.authorGuan, Yanfang-
dc.contributor.authorPotten, Christopher S-
dc.contributor.authorAllen, Terence D-
dc.contributor.authorNeurath, Markus F-
dc.contributor.authorShroyer, Noah F-
dc.contributor.authorMontrose, Marshall H-
dc.contributor.authorWatson, Alastair-
dc.date.accessioned2009-06-30T10:40:46Z-
dc.date.available2009-06-30T10:40:46Z-
dc.date.issued2007-12-
dc.identifier.citationIdentification of epithelial gaps in human small and large intestine by confocal endomicroscopy. 2007, 133 (6):1769-78 Gastroenterologyen
dc.identifier.issn1528-0012-
dc.identifier.pmid18054549-
dc.identifier.doi10.1053/j.gastro.2007.09.011-
dc.identifier.urihttp://hdl.handle.net/10541/71914-
dc.description.abstractBACKGROUND & AIMS: Confocal endomicroscopy is an emerging technology that poses the endoscopist with challenges for identifying epithelial structures in the human intestine. We have shown previously that the murine intestinal epithelium is punctuated by gaps caused by cell shedding. The goals of this study were to determine if confocal endomicroscopy could resolve the presence of human epithelial gaps and whether a proinflammatory cytokine could increase cell shedding. METHODS: Intestinal mucosa was imaged after staining with acriflavine. Confocal endomicroscopy of 17 patients yielded 6277 images from the human terminal ileum and rectum. Results were validated by parallel studies of anesthetized mice (wild-type and Math1(DeltaIntestine)) using rigid confocal probe microscopy, 2-photon/confocal microscopy, and scanning electron microscopy. RESULTS: Human terminal ileal and rectal epithelium revealed unstained areas with the diameter of an individual epithelial cell, with 2 distinct morphologies. One had a "target" appearance, shown by mouse studies to be goblet cells. The other morphology had no nucleus and was observed by rigid confocal probe microscopy and scanning electron microscopy in the villi of Math1(DeltaIntestine) mice, which lack goblet cells. In the mouse, tumor necrosis factor alpha (0.33 microg/g intraperitoneally) increases cell shedding by 27-fold and caused loss of barrier function across 20% of resultant gaps. CONCLUSIONS: Confocal endomicroscopy can distinguish between epithelial discontinuities (gaps) and goblet cells in human intestine. Results suggest that the sealing of epithelial gaps must be considered as a component of the intestinal barrier and has potential implications for intestinal barrier dysfunction in human disease.en
dc.language.isoenen
dc.subjectTumour Necrosisen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAnimals-
dc.subject.meshColonoscopy-
dc.subject.meshEpithelial Cells-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshIntestine, Large-
dc.subject.meshIntestine, Small-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMicroscopy, Confocal-
dc.subject.meshMiddle Aged-
dc.subject.meshTumor Necrosis Factor-alpha-
dc.titleIdentification of epithelial gaps in human small and large intestine by confocal endomicroscopy.en
dc.typeArticleen
dc.contributor.departmentI Medical Clinic, University of Mainz, Mainz, Germany.en
dc.identifier.journalGastroenterologyen

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