The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal

2.50
Hdl Handle:
http://hdl.handle.net/10541/71278
Title:
The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal
Authors:
Maekawa, Hiromi; Priest, Claire; Lechner, Johannes; Pereira, Gislene; Schiebel, Elmar
Abstract:
The spindle orientation checkpoint (SPOC) of budding yeast delays mitotic exit when cytoplasmic microtubules (MTs) are defective, causing the spindle to become misaligned. Delay is achieved by maintaining the activity of the Bfa1-Bub2 guanosine triphosphatase-activating protein complex, an inhibitor of mitotic exit. In this study, we show that the spindle pole body (SPB) component Spc72, a transforming acidic coiled coil-like molecule that interacts with the gamma-tubulin complex, recruits Kin4 kinase to both SPBs when cytoplasmic MTs are defective. This allows Kin4 to phosphorylate the SPB-associated Bfa1, rendering it resistant to inactivation by Cdc5 polo kinase. Consistently, forced targeting of Kin4 to both SPBs delays mitotic exit even when the anaphase spindle is correctly aligned. Moreover, we present evidence that Spc72 has an additional function in SPOC regulation that is independent of the recruitment of Kin4. Thus, Spc72 provides a missing link between cytoplasmic MT function and components of the SPOC.
Affiliation:
Zentrum für Molekulare Biologie and 2Biochemie-Zentrum, Universität Heidelberg, 69120 Heidelberg, Germany.
Citation:
The yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. 2007, 179 (3):423-36 J. Cell Biol.
Journal:
The Journal of Cell Biology
Issue Date:
5-Nov-2007
URI:
http://hdl.handle.net/10541/71278
DOI:
10.1083/jcb.200705197
PubMed ID:
17967947
Type:
Article
Language:
en
ISSN:
0021-9525
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMaekawa, Hiromi-
dc.contributor.authorPriest, Claire-
dc.contributor.authorLechner, Johannes-
dc.contributor.authorPereira, Gislene-
dc.contributor.authorSchiebel, Elmar-
dc.date.accessioned2009-06-23T13:28:39Z-
dc.date.available2009-06-23T13:28:39Z-
dc.date.issued2007-11-05-
dc.identifier.citationThe yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signal. 2007, 179 (3):423-36 J. Cell Biol.en
dc.identifier.issn0021-9525-
dc.identifier.pmid17967947-
dc.identifier.doi10.1083/jcb.200705197-
dc.identifier.urihttp://hdl.handle.net/10541/71278-
dc.description.abstractThe spindle orientation checkpoint (SPOC) of budding yeast delays mitotic exit when cytoplasmic microtubules (MTs) are defective, causing the spindle to become misaligned. Delay is achieved by maintaining the activity of the Bfa1-Bub2 guanosine triphosphatase-activating protein complex, an inhibitor of mitotic exit. In this study, we show that the spindle pole body (SPB) component Spc72, a transforming acidic coiled coil-like molecule that interacts with the gamma-tubulin complex, recruits Kin4 kinase to both SPBs when cytoplasmic MTs are defective. This allows Kin4 to phosphorylate the SPB-associated Bfa1, rendering it resistant to inactivation by Cdc5 polo kinase. Consistently, forced targeting of Kin4 to both SPBs delays mitotic exit even when the anaphase spindle is correctly aligned. Moreover, we present evidence that Spc72 has an additional function in SPOC regulation that is independent of the recruitment of Kin4. Thus, Spc72 provides a missing link between cytoplasmic MT function and components of the SPOC.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnaphase-
dc.subject.meshCell Cycle-
dc.subject.meshCell Membrane-
dc.subject.meshCentrosome-
dc.subject.meshCytoskeletal Proteins-
dc.subject.meshFungal Proteins-
dc.subject.meshMicrotubule-Associated Proteins-
dc.subject.meshMitotic Spindle Apparatus-
dc.subject.meshModels, Biological-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Kinases-
dc.subject.meshSaccharomyces cerevisiae-
dc.subject.meshSaccharomyces cerevisiae Proteins-
dc.subject.meshSignal Transduction-
dc.titleThe yeast centrosome translates the positional information of the anaphase spindle into a cell cycle signalen
dc.typeArticleen
dc.contributor.departmentZentrum für Molekulare Biologie and 2Biochemie-Zentrum, Universität Heidelberg, 69120 Heidelberg, Germany.en
dc.identifier.journalThe Journal of Cell Biologyen

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