Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/71224
Title:
Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.
Authors:
Allen, Terence D; Rutherford, Sandra A; Murray, Stephen M; Gardiner, Fiona; Kiseleva, Elena; Goldberg, Martin W; Drummond, Sheona P
Abstract:
Our previous work characterizing the biogenesis and structural integrity of the nuclear envelope and nuclear pore complexes (NPCs) has been based on amphibian material but has recently progressed into the analysis of tissue-culture cells. This protocol describes methods for the high resolution visualization, by field-emission scanning electron microscopy (FESEM), of the nucleus and associated structures in tissue culture cells. Imaging by fluorescence light microscopy shows general nuclear and NPC information at a resolution of approximately 200 nm, in contrast to the 3-5 nm resolution provided by FESEM or transmission electron microscopy (TEM), which generates detail at the macromolecular level. The protocols described here are applicable to all tissue culture cell lines tested to date (HeLa, A6, DLD, XTC and NIH 3T3). The processed cells can be stored long term under vacuum. The protocol can be completed in 5 d, including 3 d for cell growth, 1 d for processing and 1 d for imaging.
Affiliation:
Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Withington, Manchester M20 4BX, UK. tallen@picr.man.ac.uk
Citation:
Visualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells. 2007, 2 (5):1180-4 Nat Protoc
Journal:
Nature Protocols
Issue Date:
2007
URI:
http://hdl.handle.net/10541/71224
DOI:
10.1038/nprot.2007.139
PubMed ID:
17546013
Type:
Article
Language:
en
ISSN:
1750-2799
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAllen, Terence D-
dc.contributor.authorRutherford, Sandra A-
dc.contributor.authorMurray, Stephen M-
dc.contributor.authorGardiner, Fiona-
dc.contributor.authorKiseleva, Elena-
dc.contributor.authorGoldberg, Martin W-
dc.contributor.authorDrummond, Sheona P-
dc.date.accessioned2009-06-23T11:28:03Z-
dc.date.available2009-06-23T11:28:03Z-
dc.date.issued2007-
dc.identifier.citationVisualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells. 2007, 2 (5):1180-4 Nat Protocen
dc.identifier.issn1750-2799-
dc.identifier.pmid17546013-
dc.identifier.doi10.1038/nprot.2007.139-
dc.identifier.urihttp://hdl.handle.net/10541/71224-
dc.description.abstractOur previous work characterizing the biogenesis and structural integrity of the nuclear envelope and nuclear pore complexes (NPCs) has been based on amphibian material but has recently progressed into the analysis of tissue-culture cells. This protocol describes methods for the high resolution visualization, by field-emission scanning electron microscopy (FESEM), of the nucleus and associated structures in tissue culture cells. Imaging by fluorescence light microscopy shows general nuclear and NPC information at a resolution of approximately 200 nm, in contrast to the 3-5 nm resolution provided by FESEM or transmission electron microscopy (TEM), which generates detail at the macromolecular level. The protocols described here are applicable to all tissue culture cell lines tested to date (HeLa, A6, DLD, XTC and NIH 3T3). The processed cells can be stored long term under vacuum. The protocol can be completed in 5 d, including 3 d for cell growth, 1 d for processing and 1 d for imaging.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Culture Techniques-
dc.subject.meshCell Line-
dc.subject.meshCell Nucleus-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshXenopus laevis-
dc.titleVisualization of the nucleus and nuclear envelope in situ by SEM in tissue culture cells.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Withington, Manchester M20 4BX, UK. tallen@picr.man.ac.uken
dc.identifier.journalNature Protocolsen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.