Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells

2.50
Hdl Handle:
http://hdl.handle.net/10541/70468
Title:
Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells
Authors:
Welman, Arkadiusz; Griffiths, John R; Whetton, Anthony D; Dive, Caroline ( 0000-0002-1726-8850 )
Abstract:
Phosphorylation plays an important role in regulation of protein kinase C delta (PKCdelta). To date, three Ser/Thr residues (Thr 505, Ser 643, and Ser 662) and nine tyrosine residues (Tyr 52, Tyr 64, Tyr 155, Tyr 187, Tyr 311, Tyr 332, Tyr 512, Tyr 523, and Tyr 565) have been defined as regulatory phosphorylation sites for this protein (rat PKCdelta numbering). We combined doxycycline-regulated inducible gene expression technology with a hypothesis-driven mass spectrometry approach to study PKCdelta phosphorylation pattern in colorectal cancer cells. We report identification of five novel Ser/Thr phosphorylation sites: Thr 50, Thr 141, Ser 304, Thr 451, and Ser 506 (human PKCdelta numbering) following overexpression of PKCdelta in HCT116 human colon carcinoma cells grown in standard tissue culture conditions. Identification of potential novel phosphorylation sites will affect further functional studies of this protein, and may introduce additional complexity to PKCdelta signaling.
Affiliation:
Cancer Research UK, Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom. awelman@picr.man.ac.uk
Citation:
Protein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells. 2007, 16 (12):2711-5 Protein Sci.
Journal:
Protein Science
Issue Date:
Dec-2007
URI:
http://hdl.handle.net/10541/70468
DOI:
10.1110/ps.072874607
PubMed ID:
17965192
Type:
Article
Language:
en
ISSN:
0961-8368
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWelman, Arkadiusz-
dc.contributor.authorGriffiths, John R-
dc.contributor.authorWhetton, Anthony D-
dc.contributor.authorDive, Caroline-
dc.date.accessioned2009-06-15T12:16:00Z-
dc.date.available2009-06-15T12:16:00Z-
dc.date.issued2007-12-
dc.identifier.citationProtein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cells. 2007, 16 (12):2711-5 Protein Sci.en
dc.identifier.issn0961-8368-
dc.identifier.pmid17965192-
dc.identifier.doi10.1110/ps.072874607-
dc.identifier.urihttp://hdl.handle.net/10541/70468-
dc.description.abstractPhosphorylation plays an important role in regulation of protein kinase C delta (PKCdelta). To date, three Ser/Thr residues (Thr 505, Ser 643, and Ser 662) and nine tyrosine residues (Tyr 52, Tyr 64, Tyr 155, Tyr 187, Tyr 311, Tyr 332, Tyr 512, Tyr 523, and Tyr 565) have been defined as regulatory phosphorylation sites for this protein (rat PKCdelta numbering). We combined doxycycline-regulated inducible gene expression technology with a hypothesis-driven mass spectrometry approach to study PKCdelta phosphorylation pattern in colorectal cancer cells. We report identification of five novel Ser/Thr phosphorylation sites: Thr 50, Thr 141, Ser 304, Thr 451, and Ser 506 (human PKCdelta numbering) following overexpression of PKCdelta in HCT116 human colon carcinoma cells grown in standard tissue culture conditions. Identification of potential novel phosphorylation sites will affect further functional studies of this protein, and may introduce additional complexity to PKCdelta signaling.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshCatalytic Domain-
dc.subject.meshColorectal Neoplasms-
dc.subject.meshDoxycycline-
dc.subject.meshHumans-
dc.subject.meshMass Spectrometry-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPhosphorylation-
dc.subject.meshProtein Kinase C-delta-
dc.subject.meshSerine-
dc.subject.meshThreonine-
dc.titleProtein kinase C delta is phosphorylated on five novel Ser/Thr sites following inducible overexpression in human colorectal cancer cellsen
dc.typeArticleen
dc.contributor.departmentCancer Research UK, Clinical and Experimental Pharmacology Group, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom. awelman@picr.man.ac.uken
dc.identifier.journalProtein Scienceen

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