ATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation.

2.50
Hdl Handle:
http://hdl.handle.net/10541/70319
Title:
ATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation.
Authors:
Bruhat, Alain; Chérasse, Yoan; Maurin, Anne-Catherine; Breitwieser, Wolfgang; Parry, Laurent; Deval, Christiane; Jones, Nic; Jousse, Céline; Fafournoux, Pierre
Abstract:
The transcriptional activation of CHOP (a CCAAT/enhancer-binding protein-related gene) by amino acid deprivation involves the activating transcription factor 2 (ATF2) and the activating transcription factor 4 (ATF4) binding the amino acid response element (AARE) within the promoter. Using a chromatin immunoprecipitation approach, we report that in vivo binding of phospho-ATF2 and ATF4 to CHOP AARE are associated with acetylation of histones H4 and H2B in response to amino acid starvation. A time course analysis reveals that ATF2 phosphorylation precedes histone acetylation, ATF4 binding and the increase in CHOP mRNA. We also show that ATF4 binding and histone acetylation are two independent events that are required for the CHOP induction upon amino acid starvation. Using ATF2-deficient mouse embryonic fibroblasts, we demonstrate that ATF2 is essential in the acetylation of histone H4 and H2B in vivo. The role of ATF2 on histone H4 acetylation is dependent on its binding to the AARE and can be extended to other amino acid regulated genes. Thus, ATF2 is involved in promoting the modification of the chromatin structure to enhance the transcription of a number of amino acid-regulated genes.
Affiliation:
UMR 1019, Unité de Nutrition Humaine, INRA de Theix, 63122 Saint Genès Champanelle, France. bruhat@clermont.inra.fr
Citation:
ATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation. 2007, 35 (4):1312-21 Nucleic Acids Res.
Journal:
Nucleic Acids Research
Issue Date:
2007
URI:
http://hdl.handle.net/10541/70319
DOI:
10.1093/nar/gkm038
PubMed ID:
17267404
Type:
Article
Language:
en
ISSN:
1362-4962
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBruhat, Alain-
dc.contributor.authorChérasse, Yoan-
dc.contributor.authorMaurin, Anne-Catherine-
dc.contributor.authorBreitwieser, Wolfgang-
dc.contributor.authorParry, Laurent-
dc.contributor.authorDeval, Christiane-
dc.contributor.authorJones, Nic-
dc.contributor.authorJousse, Céline-
dc.contributor.authorFafournoux, Pierre-
dc.date.accessioned2009-06-12T13:39:36Z-
dc.date.available2009-06-12T13:39:36Z-
dc.date.issued2007-
dc.identifier.citationATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation. 2007, 35 (4):1312-21 Nucleic Acids Res.en
dc.identifier.issn1362-4962-
dc.identifier.pmid17267404-
dc.identifier.doi10.1093/nar/gkm038-
dc.identifier.urihttp://hdl.handle.net/10541/70319-
dc.description.abstractThe transcriptional activation of CHOP (a CCAAT/enhancer-binding protein-related gene) by amino acid deprivation involves the activating transcription factor 2 (ATF2) and the activating transcription factor 4 (ATF4) binding the amino acid response element (AARE) within the promoter. Using a chromatin immunoprecipitation approach, we report that in vivo binding of phospho-ATF2 and ATF4 to CHOP AARE are associated with acetylation of histones H4 and H2B in response to amino acid starvation. A time course analysis reveals that ATF2 phosphorylation precedes histone acetylation, ATF4 binding and the increase in CHOP mRNA. We also show that ATF4 binding and histone acetylation are two independent events that are required for the CHOP induction upon amino acid starvation. Using ATF2-deficient mouse embryonic fibroblasts, we demonstrate that ATF2 is essential in the acetylation of histone H4 and H2B in vivo. The role of ATF2 on histone H4 acetylation is dependent on its binding to the AARE and can be extended to other amino acid regulated genes. Thus, ATF2 is involved in promoting the modification of the chromatin structure to enhance the transcription of a number of amino acid-regulated genes.en
dc.language.isoenen
dc.subject.meshAcetylation-
dc.subject.meshActivating Transcription Factor 2-
dc.subject.meshActivating Transcription Factor 4-
dc.subject.meshAmino Acids-
dc.subject.meshAnimals-
dc.subject.meshCells, Cultured-
dc.subject.meshHistones-
dc.subject.meshMice-
dc.subject.meshPhosphorylation-
dc.subject.meshResponse Elements-
dc.subject.meshTranscription Factor CHOP-
dc.subject.meshTranscriptional Activation-
dc.titleATF2 is required for amino acid-regulated transcription by orchestrating specific histone acetylation.en
dc.typeArticleen
dc.contributor.departmentUMR 1019, Unité de Nutrition Humaine, INRA de Theix, 63122 Saint Genès Champanelle, France. bruhat@clermont.inra.fren
dc.identifier.journalNucleic Acids Researchen

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