Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer

2.50
Hdl Handle:
http://hdl.handle.net/10541/70266
Title:
Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer
Authors:
Byrne, Ged J; Hayden, K; McDowell, G; Lang, H; Kirwan, C C; Tetlow, L C; Kumar, Shant; Bundred, Nigel J
Abstract:
In cancer models, thrombospondin-1 (TSP-1) has been shown to inhibit angiogenesis or promote metastasis by increasing adhesion of malignant cells to endothelium. To determine the role of TSP-1 in breast cancer and breast cancer angiogenesis, we have measured TSP-1 in plasma and tumour cytosols and compared levels to established clinicopathological prognostic parameters and intratumoural microvessel density. TSP-1 was measured, by radioimmunoassay, in plasma (pTSP-1) and tumour cytosols (cTSP-1) of women with early breast cancer (EBC) (n=71). pTSP-1 in EBC was compared to pTSP-1 levels in women with advanced breast cancer (ABC) (n=66), normal controls (n=77) and was correlated with prognostic features and microvessel density (MVD) (measured by CD31 immunostaining). cTSP-1 levels were compared to prognostic features and microvessel density. pTSP-1 in women with EBC (median 484, IQR 344-877 ng/ml) and ABC (median 588, IQR 430-952 ng/ml) were elevated when compared to normal controls (median 21, IQR 175-247) (p<0.001). Women with lymph node metastases (n=35) had higher levels of TSP-1 (median 799 ng/ml, IQR 455-943) than women who were node negative (median 343 ng/ml, IQR 267-514) (n=36) (p<0.05). Levels of pTSP-1 in EBC correlated with MVD (R=0.39, p<0.05). Levels of TSP-1 in tumour cytosols of women with EBC (median 1714, IQR 893-5283 ng/ml) correlated with microvessel density (R=0.46, p<0.01). Circulating levels of TSP-1 appear to be a marker of breast cancer aggressiveness and in breast cancer may have a pro-angiogenic rather than anti-angiogenic role.
Affiliation:
Department of Surgery, South Manchester University Hospitals, Wythenshawe Hospital, Manchester, UK.
Citation:
Angiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer. 2007, 31 (5):1127-32 Int. J. Oncol.
Journal:
International Journal of Oncology
Issue Date:
Nov-2007
URI:
http://hdl.handle.net/10541/70266
PubMed ID:
17912439
Type:
Article
Language:
en
ISSN:
1019-6439
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorByrne, Ged J-
dc.contributor.authorHayden, K-
dc.contributor.authorMcDowell, G-
dc.contributor.authorLang, H-
dc.contributor.authorKirwan, C C-
dc.contributor.authorTetlow, L C-
dc.contributor.authorKumar, Shant-
dc.contributor.authorBundred, Nigel J-
dc.date.accessioned2009-06-12T10:39:20Z-
dc.date.available2009-06-12T10:39:20Z-
dc.date.issued2007-11-
dc.identifier.citationAngiogenic characteristics of circulating and tumoural thrombospondin-1 in breast cancer. 2007, 31 (5):1127-32 Int. J. Oncol.en
dc.identifier.issn1019-6439-
dc.identifier.pmid17912439-
dc.identifier.urihttp://hdl.handle.net/10541/70266-
dc.description.abstractIn cancer models, thrombospondin-1 (TSP-1) has been shown to inhibit angiogenesis or promote metastasis by increasing adhesion of malignant cells to endothelium. To determine the role of TSP-1 in breast cancer and breast cancer angiogenesis, we have measured TSP-1 in plasma and tumour cytosols and compared levels to established clinicopathological prognostic parameters and intratumoural microvessel density. TSP-1 was measured, by radioimmunoassay, in plasma (pTSP-1) and tumour cytosols (cTSP-1) of women with early breast cancer (EBC) (n=71). pTSP-1 in EBC was compared to pTSP-1 levels in women with advanced breast cancer (ABC) (n=66), normal controls (n=77) and was correlated with prognostic features and microvessel density (MVD) (measured by CD31 immunostaining). cTSP-1 levels were compared to prognostic features and microvessel density. pTSP-1 in women with EBC (median 484, IQR 344-877 ng/ml) and ABC (median 588, IQR 430-952 ng/ml) were elevated when compared to normal controls (median 21, IQR 175-247) (p<0.001). Women with lymph node metastases (n=35) had higher levels of TSP-1 (median 799 ng/ml, IQR 455-943) than women who were node negative (median 343 ng/ml, IQR 267-514) (n=36) (p<0.05). Levels of pTSP-1 in EBC correlated with MVD (R=0.39, p<0.05). Levels of TSP-1 in tumour cytosols of women with EBC (median 1714, IQR 893-5283 ng/ml) correlated with microvessel density (R=0.46, p<0.01). Circulating levels of TSP-1 appear to be a marker of breast cancer aggressiveness and in breast cancer may have a pro-angiogenic rather than anti-angiogenic role.en
dc.language.isoenen
dc.subjectBreast Canceren
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshBreast Neoplasms-
dc.subject.meshCytosol-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMiddle Aged-
dc.subject.meshNeovascularization, Pathologic-
dc.subject.meshThrombospondin 1-
dc.titleAngiogenic characteristics of circulating and tumoural thrombospondin-1 in breast canceren
dc.typeArticleen
dc.contributor.departmentDepartment of Surgery, South Manchester University Hospitals, Wythenshawe Hospital, Manchester, UK.en
dc.identifier.journalInternational Journal of Oncologyen

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