Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis.

2.50
Hdl Handle:
http://hdl.handle.net/10541/70158
Title:
Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis.
Authors:
Wang, Qiuyu; Kumar, Shant; Mitsios, Nick; Slevin, Mark; Kumar, Patricia
Abstract:
PAX3 encodes a transcription factor, which with Zic1 is necessary for induction of the neural crest during early embryonic development. There are 7 human PAX3 isoforms (a-h). PAX3e is the full length isoform comprising 10 exons. PAX3c comprises 8 exons plus 5 codons of intron 8, while PAX3g has a truncated transactivation domain. Previous studies by us indicated that these isoforms have different activities in melanocytes in vitro. In this study, a mouse gene oligo array ( approximately 7.5 k oligos), from the Human Genome Mapping Project (HGMP) Resource Centre, was used to screen for alterations in downstream gene expression in PAX3c, PAX3e and PAX3g melanocyte transfectants, compared with empty vector controls. The data analyses identified 109 genes up or downregulated, at least 2-fold, and involved in cell differentiation, proliferation, migration, adhesion, apoptosis and angiogenesis. Semi-quantitative RT-PCR and Western blotting confirmed the changes identified by microarrays for several putative targets of PAX3, including Met, MyoD and Muc18, and previously undescribed targets, including Dhh, Fgf17, Kitl and Rac1. Thus, our data reveal that PAX3 isoforms regulate distinct but overlapping sets of genes in melanocytes in vitro.
Affiliation:
School of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.
Citation:
Investigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis. 2007, 120 (6):1223-31 Int. J. Cancer
Journal:
International Journal of Cancer.
Issue Date:
15-Mar-2007
URI:
http://hdl.handle.net/10541/70158
DOI:
10.1002/ijc.22316
PubMed ID:
17187370
Type:
Article
Language:
en
ISSN:
0020-7136
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorWang, Qiuyu-
dc.contributor.authorKumar, Shant-
dc.contributor.authorMitsios, Nick-
dc.contributor.authorSlevin, Mark-
dc.contributor.authorKumar, Patricia-
dc.date.accessioned2009-06-11T11:14:46Z-
dc.date.available2009-06-11T11:14:46Z-
dc.date.issued2007-03-15-
dc.identifier.citationInvestigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis. 2007, 120 (6):1223-31 Int. J. Canceren
dc.identifier.issn0020-7136-
dc.identifier.pmid17187370-
dc.identifier.doi10.1002/ijc.22316-
dc.identifier.urihttp://hdl.handle.net/10541/70158-
dc.description.abstractPAX3 encodes a transcription factor, which with Zic1 is necessary for induction of the neural crest during early embryonic development. There are 7 human PAX3 isoforms (a-h). PAX3e is the full length isoform comprising 10 exons. PAX3c comprises 8 exons plus 5 codons of intron 8, while PAX3g has a truncated transactivation domain. Previous studies by us indicated that these isoforms have different activities in melanocytes in vitro. In this study, a mouse gene oligo array ( approximately 7.5 k oligos), from the Human Genome Mapping Project (HGMP) Resource Centre, was used to screen for alterations in downstream gene expression in PAX3c, PAX3e and PAX3g melanocyte transfectants, compared with empty vector controls. The data analyses identified 109 genes up or downregulated, at least 2-fold, and involved in cell differentiation, proliferation, migration, adhesion, apoptosis and angiogenesis. Semi-quantitative RT-PCR and Western blotting confirmed the changes identified by microarrays for several putative targets of PAX3, including Met, MyoD and Muc18, and previously undescribed targets, including Dhh, Fgf17, Kitl and Rac1. Thus, our data reveal that PAX3 isoforms regulate distinct but overlapping sets of genes in melanocytes in vitro.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Movement-
dc.subject.meshGene Expression Profiling-
dc.subject.meshGene Expression Regulation-
dc.subject.meshHumans-
dc.subject.meshMelanocytes-
dc.subject.meshMice-
dc.subject.meshNeovascularization, Physiologic-
dc.subject.meshOligonucleotide Array Sequence Analysis-
dc.subject.meshPaired Box Transcription Factors-
dc.subject.meshProtein Isoforms-
dc.subject.meshRNA, Messenger-
dc.subject.meshTransfection-
dc.titleInvestigation of downstream target genes of PAX3c, PAX3e and PAX3g isoforms in melanocytes by microarray analysis.en
dc.typeArticleen
dc.contributor.departmentSchool of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, United Kingdom.en
dc.identifier.journalInternational Journal of Cancer.en

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