NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.

2.50
Hdl Handle:
http://hdl.handle.net/10541/68739
Title:
NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.
Authors:
Salpingidou, Georgia; Rzepecki, Ryszard; Kiseleva, Elena; Lyon, Carol; Lane, Birgit; Fusiek, Kasia; Golebiewska, Anja; Drummond, Sheona P; Allen, Terence D; Ellis, Juliet A; Smythe, Carl; Goldberg, Martin W; Hutchison, Christopher J
Abstract:
In vertebrates, the nuclear envelope (NE) assembles and disassembles during mitosis. As the NE is a complex structure consisting of inner and outer membranes, nuclear pore complexes (NPCs) and the nuclear lamina, NE assembly must be a controlled and systematic process. In Xenopus egg extracts, NE assembly is mediated by two distinct membrane vesicle populations, termed NEP-A and NEP-B. Here, we re-investigate how these two membrane populations contribute to NPC assembly. In growing stage III Xenopus oocytes, NPC assembly intermediates are frequently observed. High concentrations of NPC assembly intermediates always correlate with fusion of vesicles into preformed membranes. In Xenopus egg extracts, two integral membrane proteins essential for NPC assembly, POM121 and NDC1, are exclusively associated with NEP-B membranes. By contrast, a third integral membrane protein associated with the NPCs, gp210, associates only with NEP-A membranes. During NE assembly, fusion between NEP-A and NEP-B led to the formation of fusion junctions at which >65% of assembling NPCs were located. To investigate how each membrane type contributes to NPC assembly, we preferentially limited NEP-A in NE assembly assays. We found that, by limiting the NEP-A contribution to the NE, partially formed NPCs were assembled in which protein components of the nucleoplasmic face were depleted or absent. Our data suggest that fusion between NEP-A and NEP-B membranes is essential for NPC assembly and that, in contrast to previous reports, both membranes contribute to NPC assembly.
Affiliation:
Integrative Cell Biology Laboratories, School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham DH1 3LE, UK.
Citation:
NEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes. 2008, 121 (Pt 5):706-16 J. Cell. Sci.
Journal:
Journal of Cell Science
Issue Date:
1-Mar-2008
URI:
http://hdl.handle.net/10541/68739
DOI:
10.1242/jcs.019968
PubMed ID:
18270266
Type:
Article
Language:
en
ISSN:
0021-9533
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSalpingidou, Georgia-
dc.contributor.authorRzepecki, Ryszard-
dc.contributor.authorKiseleva, Elena-
dc.contributor.authorLyon, Carol-
dc.contributor.authorLane, Birgit-
dc.contributor.authorFusiek, Kasia-
dc.contributor.authorGolebiewska, Anja-
dc.contributor.authorDrummond, Sheona P-
dc.contributor.authorAllen, Terence D-
dc.contributor.authorEllis, Juliet A-
dc.contributor.authorSmythe, Carl-
dc.contributor.authorGoldberg, Martin W-
dc.contributor.authorHutchison, Christopher J-
dc.date.accessioned2009-05-21T16:39:43Z-
dc.date.available2009-05-21T16:39:43Z-
dc.date.issued2008-03-01-
dc.identifier.citationNEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes. 2008, 121 (Pt 5):706-16 J. Cell. Sci.en
dc.identifier.issn0021-9533-
dc.identifier.pmid18270266-
dc.identifier.doi10.1242/jcs.019968-
dc.identifier.urihttp://hdl.handle.net/10541/68739-
dc.description.abstractIn vertebrates, the nuclear envelope (NE) assembles and disassembles during mitosis. As the NE is a complex structure consisting of inner and outer membranes, nuclear pore complexes (NPCs) and the nuclear lamina, NE assembly must be a controlled and systematic process. In Xenopus egg extracts, NE assembly is mediated by two distinct membrane vesicle populations, termed NEP-A and NEP-B. Here, we re-investigate how these two membrane populations contribute to NPC assembly. In growing stage III Xenopus oocytes, NPC assembly intermediates are frequently observed. High concentrations of NPC assembly intermediates always correlate with fusion of vesicles into preformed membranes. In Xenopus egg extracts, two integral membrane proteins essential for NPC assembly, POM121 and NDC1, are exclusively associated with NEP-B membranes. By contrast, a third integral membrane protein associated with the NPCs, gp210, associates only with NEP-A membranes. During NE assembly, fusion between NEP-A and NEP-B led to the formation of fusion junctions at which >65% of assembling NPCs were located. To investigate how each membrane type contributes to NPC assembly, we preferentially limited NEP-A in NE assembly assays. We found that, by limiting the NEP-A contribution to the NE, partially formed NPCs were assembled in which protein components of the nucleoplasmic face were depleted or absent. Our data suggest that fusion between NEP-A and NEP-B membranes is essential for NPC assembly and that, in contrast to previous reports, both membranes contribute to NPC assembly.en
dc.language.isoenen
dc.subjectNuclear Envelope Assemblyen
dc.subjectLAP2en
dc.subject.meshActive Transport, Cell Nucleus-
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus-
dc.subject.meshCytoplasmic Vesicles-
dc.subject.meshFemale-
dc.subject.meshMacromolecular Substances-
dc.subject.meshMembrane Proteins-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Pore-
dc.subject.meshNuclear Pore Complex Proteins-
dc.subject.meshNuclear Proteins-
dc.subject.meshOocytes-
dc.subject.meshXenopus Proteins-
dc.subject.meshXenopus laevis-
dc.titleNEP-A and NEP-B both contribute to nuclear pore formation in Xenopus eggs and oocytes.en
dc.typeArticleen
dc.contributor.departmentIntegrative Cell Biology Laboratories, School of Biological and Biomedical Sciences, The University of Durham, South Road, Durham DH1 3LE, UK.en
dc.identifier.journalJournal of Cell Scienceen

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