Leukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke.

2.50
Hdl Handle:
http://hdl.handle.net/10541/68193
Title:
Leukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke.
Authors:
Slevin, Mark; Krupinski, Jerzy; Mitsios, Nick; Perikleous, C; Cuadrado, E; Montaner, J; Sanfeliu, Coral; Luque, A; Kumar, Shant; Kumar, Patricia; Gaffney, John
Abstract:
Leukaemia inhibitory factor (LIF) is a glycoprotein of the interleukin-6 family, which has potent pro-inflammatory properties and is involved in regulation of neuronal differentiation. We have previously identified its upregulation in gene microarrays following acute ischaemic stroke in man. LIF expression and localization was measured in human ischaemic stroke autopsy specimens, in a rat model of middle cerebral artery occlusion (MCAO) and in human foetal neural cell cultures following oxygen-glucose deprivation (OGD) by Western blotting and immunohistochemistry. Circulating LIF was determined in the plasma of patients in the hyper-acute stroke phase using a multiplex enzyme-linked-immunosorbent serologic assay system. Patients demonstrated an increase in LIF expression in peri-infarcted regions with localization in neurons and endothelial cells of microvessels surrounding the infarcted core. The rat MCAO model showed similar upregulation in neurons with a peak increase at 90 min. Circulating serum LIF expression was significantly decreased in the hyper-acute phase of stroke. Brain-derived neurons and glia cultured in vitro demonstrated an increase in gene/protein and protein expression respectively following exposure to OGD. Increased LIF expression in peri-infarcted regions and sequestration from the peripheral circulation in acute stroke patients are characteristic of the pathobiological response to ischaemia and tissue damage.
Affiliation:
The Department of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK. m.a.slevin@mmu.ac.uk
Citation:
Leukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke. 2008, 15 (1):29-37 Eur. J. Neurol.
Journal:
European Journal of Neurology
Issue Date:
Jan-2008
URI:
http://hdl.handle.net/10541/68193
DOI:
10.1111/j.1468-1331.2007.01995.x
PubMed ID:
18042242
Type:
Article
Language:
en
ISSN:
1468-1331
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorSlevin, Mark-
dc.contributor.authorKrupinski, Jerzy-
dc.contributor.authorMitsios, Nick-
dc.contributor.authorPerikleous, C-
dc.contributor.authorCuadrado, E-
dc.contributor.authorMontaner, J-
dc.contributor.authorSanfeliu, Coral-
dc.contributor.authorLuque, A-
dc.contributor.authorKumar, Shant-
dc.contributor.authorKumar, Patricia-
dc.contributor.authorGaffney, John-
dc.date.accessioned2009-05-14T13:45:39Z-
dc.date.available2009-05-14T13:45:39Z-
dc.date.issued2008-01-
dc.identifier.citationLeukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke. 2008, 15 (1):29-37 Eur. J. Neurol.en
dc.identifier.issn1468-1331-
dc.identifier.pmid18042242-
dc.identifier.doi10.1111/j.1468-1331.2007.01995.x-
dc.identifier.urihttp://hdl.handle.net/10541/68193-
dc.description.abstractLeukaemia inhibitory factor (LIF) is a glycoprotein of the interleukin-6 family, which has potent pro-inflammatory properties and is involved in regulation of neuronal differentiation. We have previously identified its upregulation in gene microarrays following acute ischaemic stroke in man. LIF expression and localization was measured in human ischaemic stroke autopsy specimens, in a rat model of middle cerebral artery occlusion (MCAO) and in human foetal neural cell cultures following oxygen-glucose deprivation (OGD) by Western blotting and immunohistochemistry. Circulating LIF was determined in the plasma of patients in the hyper-acute stroke phase using a multiplex enzyme-linked-immunosorbent serologic assay system. Patients demonstrated an increase in LIF expression in peri-infarcted regions with localization in neurons and endothelial cells of microvessels surrounding the infarcted core. The rat MCAO model showed similar upregulation in neurons with a peak increase at 90 min. Circulating serum LIF expression was significantly decreased in the hyper-acute phase of stroke. Brain-derived neurons and glia cultured in vitro demonstrated an increase in gene/protein and protein expression respectively following exposure to OGD. Increased LIF expression in peri-infarcted regions and sequestration from the peripheral circulation in acute stroke patients are characteristic of the pathobiological response to ischaemia and tissue damage.en
dc.language.isoenen
dc.subjectGliaen
dc.subjectIschaemic Strokeen
dc.subjectNeuronalen
dc.subjectPlasmaen
dc.subject.meshAcute Disease-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshAnimals-
dc.subject.meshBrain-
dc.subject.meshBrain Ischemia-
dc.subject.meshCells, Cultured-
dc.subject.meshCoculture Techniques-
dc.subject.meshDisease Models, Animal-
dc.subject.meshDisease Progression-
dc.subject.meshEndothelial Cells-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshInfarction, Middle Cerebral Artery-
dc.subject.meshLeukemia Inhibitory Factor-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshNeuroglia-
dc.subject.meshNeurons-
dc.subject.meshRats-
dc.subject.meshRats, Sprague-Dawley-
dc.subject.meshStroke-
dc.subject.meshUp-Regulation-
dc.titleLeukaemia inhibitory factor is over-expressed by ischaemic brain tissue concomitant with reduced plasma expression following acute stroke.en
dc.typeArticleen
dc.contributor.departmentThe Department of Biology, Chemistry and Health Science, Manchester Metropolitan University, Manchester, UK. m.a.slevin@mmu.ac.uken
dc.identifier.journalEuropean Journal of Neurologyen

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