2.50
Hdl Handle:
http://hdl.handle.net/10541/68173
Title:
Scanning electron microscopy of nuclear structure.
Authors:
Allen, Terence D; Rutherford, Sandra A; Murray, Stephen M; Drummond, Sheona P; Goldberg, Martin W; Kiseleva, Elena
Abstract:
Accessing internal structure and retaining relative three dimensional (3D) organization within the nucleus has always proved difficult in the electron microscope. This is due to the overall size and largely fibrous nature of the contents, making large scale 3D reconstructions difficult from thin sections using transmission electron microscopy. This chapter brings together a number of methods developed for visualization of nuclear structure by scanning electron microscopy (SEM). These methods utilize the easily accessed high resolution available in field emission instruments. Surface imaging has proved particularly useful to date in studies of the nuclear envelope and pore complexes, and has also shown promise for internal nuclear organization, including the dynamic and radical reorganization of structure during cell division. Consequently, surface imaging in the SEM has the potential to make a significant contribution to our understanding of nuclear structure.
Affiliation:
Department of Structural Cell Biology, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom.
Citation:
Scanning electron microscopy of nuclear structure. 2008, 88:389-409 Methods Cell Biol.
Journal:
Methods in Cell Biology
Issue Date:
2008
URI:
http://hdl.handle.net/10541/68173
DOI:
10.1016/S0091-679X(08)00420-2
PubMed ID:
18617044
Type:
Book chapter
Language:
en
ISSN:
0091-679X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAllen, Terence D-
dc.contributor.authorRutherford, Sandra A-
dc.contributor.authorMurray, Stephen M-
dc.contributor.authorDrummond, Sheona P-
dc.contributor.authorGoldberg, Martin W-
dc.contributor.authorKiseleva, Elena-
dc.date.accessioned2009-05-14T10:12:41Z-
dc.date.available2009-05-14T10:12:41Z-
dc.date.issued2008-
dc.identifier.citationScanning electron microscopy of nuclear structure. 2008, 88:389-409 Methods Cell Biol.en
dc.identifier.issn0091-679X-
dc.identifier.pmid18617044-
dc.identifier.doi10.1016/S0091-679X(08)00420-2-
dc.identifier.urihttp://hdl.handle.net/10541/68173-
dc.description.abstractAccessing internal structure and retaining relative three dimensional (3D) organization within the nucleus has always proved difficult in the electron microscope. This is due to the overall size and largely fibrous nature of the contents, making large scale 3D reconstructions difficult from thin sections using transmission electron microscopy. This chapter brings together a number of methods developed for visualization of nuclear structure by scanning electron microscopy (SEM). These methods utilize the easily accessed high resolution available in field emission instruments. Surface imaging has proved particularly useful to date in studies of the nuclear envelope and pore complexes, and has also shown promise for internal nuclear organization, including the dynamic and radical reorganization of structure during cell division. Consequently, surface imaging in the SEM has the potential to make a significant contribution to our understanding of nuclear structure.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Nucleus-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshOocytes-
dc.subject.meshXenopus laevis-
dc.titleScanning electron microscopy of nuclear structure.en
dc.typeBook chapteren
dc.contributor.departmentDepartment of Structural Cell Biology, Paterson Institute for Cancer Research, University of Manchester, Manchester M20 4BX, United Kingdom.en
dc.identifier.journalMethods in Cell Biologyen
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