Trials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/66863
Title:
Trials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors.
Authors:
Iczkowski, Kenneth A; Butler, Samantha L; Shanks, Jonathan H; Hossain, Deloar; Schall, Albrecht; Meiers, Isabelle; Zhou, Ming; Torkko, Kathleen C; Kim, Stacy J; MacLennan, Gregory T
Abstract:
Organic cation transporter 3/4 (OCT3/4) is a transcription factor of embryonic stem cells; c-kit (CD117) is a tyrosine kinase receptor implicated in seminoma carcinogenesis. Their reactivity is well characterized in testicular, but not extragonadal and metastatic, germ cell tumors. A total of 93 germ cell tumors (41 seminoma, 22 embryonal carcinoma, 18 teratoma, and 12 yolk sac tumor) were obtained from the central nervous system (30), mediastinum (23), retroperitoneum/abdomen (31), and other locations (9). Immunohistochemical staining for c-kit, placental-like alkaline phosphatase (PLAP), OCT3/4, and new markers D2-40 and AP-2gamma was performed on seminomas; CD30 and epithelial membrane antigen were added for nonseminomas. In embryonal carcinoma, c-kit reacted in 17 of 22 cases, OCT3/4 in 18 of 22, and PLAP in 13 of 22. OCT3/4 was superior to PLAP in intensity and percent cells staining. In seminoma, OCT3/4 and D2-40 were superior to PLAP in intensity and percent cells; c-kit and AP-2gamma were superior in percent cells. D2-40 stained 23 of 24 seminomas strongly but had only weak focal reactivity in 6 of 17 embryonal carcinomas. Sensitivity and specificity were high for OCT3/4 discriminating seminoma and embryonal carcinoma, and c-kit discriminating seminoma, from other germ cell tumors. For embryonal carcinoma, OCT3/4 had higher specificity (0.94) than CD30 (0.786) owing to CD30 reactivity in 3 of 10 teratomas. Epithelial membrane antigen discriminated teratoma from other nonseminomas with a sensitivity of 1 but reacted occasionally in embryonal carcinoma (3/15) and yolk sac tumor (2/7). In conclusion, for extragonadal seminoma, OCT3/4, AP-2gamma, D2-40, and c-kit were equivalently superior to PLAP. For embryonal carcinoma, OCT3/4 was superior to PLAP and more specific than CD30. D2-40 is recommended to discriminate between seminoma and embryonal carcinoma.
Affiliation:
Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL 32608, USA. kenneth.iczkowski@uchsc.edu
Citation:
Trials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors. 2008, 39 (2):275-81 Hum. Pathol.
Journal:
Human Pathology
Issue Date:
Feb-2008
URI:
http://hdl.handle.net/10541/66863
DOI:
10.1016/j.humpath.2007.07.002
PubMed ID:
18045648
Type:
Article
Language:
en
ISSN:
0046-8177
Appears in Collections:
All Christie Publications ; Pathology

Full metadata record

DC FieldValue Language
dc.contributor.authorIczkowski, Kenneth A-
dc.contributor.authorButler, Samantha L-
dc.contributor.authorShanks, Jonathan H-
dc.contributor.authorHossain, Deloar-
dc.contributor.authorSchall, Albrecht-
dc.contributor.authorMeiers, Isabelle-
dc.contributor.authorZhou, Ming-
dc.contributor.authorTorkko, Kathleen C-
dc.contributor.authorKim, Stacy J-
dc.contributor.authorMacLennan, Gregory T-
dc.date.accessioned2009-05-01T10:39:20Z-
dc.date.available2009-05-01T10:39:20Z-
dc.date.issued2008-02-
dc.identifier.citationTrials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors. 2008, 39 (2):275-81 Hum. Pathol.en
dc.identifier.issn0046-8177-
dc.identifier.pmid18045648-
dc.identifier.doi10.1016/j.humpath.2007.07.002-
dc.identifier.urihttp://hdl.handle.net/10541/66863-
dc.description.abstractOrganic cation transporter 3/4 (OCT3/4) is a transcription factor of embryonic stem cells; c-kit (CD117) is a tyrosine kinase receptor implicated in seminoma carcinogenesis. Their reactivity is well characterized in testicular, but not extragonadal and metastatic, germ cell tumors. A total of 93 germ cell tumors (41 seminoma, 22 embryonal carcinoma, 18 teratoma, and 12 yolk sac tumor) were obtained from the central nervous system (30), mediastinum (23), retroperitoneum/abdomen (31), and other locations (9). Immunohistochemical staining for c-kit, placental-like alkaline phosphatase (PLAP), OCT3/4, and new markers D2-40 and AP-2gamma was performed on seminomas; CD30 and epithelial membrane antigen were added for nonseminomas. In embryonal carcinoma, c-kit reacted in 17 of 22 cases, OCT3/4 in 18 of 22, and PLAP in 13 of 22. OCT3/4 was superior to PLAP in intensity and percent cells staining. In seminoma, OCT3/4 and D2-40 were superior to PLAP in intensity and percent cells; c-kit and AP-2gamma were superior in percent cells. D2-40 stained 23 of 24 seminomas strongly but had only weak focal reactivity in 6 of 17 embryonal carcinomas. Sensitivity and specificity were high for OCT3/4 discriminating seminoma and embryonal carcinoma, and c-kit discriminating seminoma, from other germ cell tumors. For embryonal carcinoma, OCT3/4 had higher specificity (0.94) than CD30 (0.786) owing to CD30 reactivity in 3 of 10 teratomas. Epithelial membrane antigen discriminated teratoma from other nonseminomas with a sensitivity of 1 but reacted occasionally in embryonal carcinoma (3/15) and yolk sac tumor (2/7). In conclusion, for extragonadal seminoma, OCT3/4, AP-2gamma, D2-40, and c-kit were equivalently superior to PLAP. For embryonal carcinoma, OCT3/4 was superior to PLAP and more specific than CD30. D2-40 is recommended to discriminate between seminoma and embryonal carcinoma.en
dc.language.isoenen
dc.subjectCancer Proteinsen
dc.subjectOvarian Canceren
dc.subjectTesticular Canceren
dc.subjectTumour Markersen
dc.subjectGerm Cell Tumouren
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshFemale-
dc.subject.meshFluorescent Antibody Technique, Indirect-
dc.subject.meshGerminoma-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshNeoplasm Proteins-
dc.subject.meshOctamer Transcription Factor-3-
dc.subject.meshOvarian Neoplasms-
dc.subject.meshSensitivity and Specificity-
dc.subject.meshTesticular Neoplasms-
dc.subject.meshTranscription Factor AP-2-
dc.subject.meshTumor Markers, Biological-
dc.titleTrials of new germ cell immunohistochemical stains in 93 extragonadal and metastatic germ cell tumors.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, Immunology, and Laboratory Medicine, University of Florida, Gainesville, FL 32608, USA. kenneth.iczkowski@uchsc.eduen
dc.identifier.journalHuman Pathologyen
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