Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.

2.50
Hdl Handle:
http://hdl.handle.net/10541/66076
Title:
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.
Authors:
Sanchez-Diaz, Alberto; Marchesi, Vanessa; Murray, Stephen M; Jones, Richard C; Pereira, Gislene; Edmondson, Ricky D; Allen, Terence D; Labib, Karim
Abstract:
By rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.
Affiliation:
Cancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.
Citation:
Inn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. 2008, 10 (4):395-406 Nat. Cell Biol.
Journal:
Nature Cell Biology
Issue Date:
Apr-2008
URI:
http://hdl.handle.net/10541/66076
DOI:
10.1038/ncb1701
PubMed ID:
18344988
Type:
Article
Language:
en
ISSN:
1476-4679
Appears in Collections:
All Paterson Institute for Cancer Research; Cell Cycle Group

Full metadata record

DC FieldValue Language
dc.contributor.authorSanchez-Diaz, Alberto-
dc.contributor.authorMarchesi, Vanessa-
dc.contributor.authorMurray, Stephen M-
dc.contributor.authorJones, Richard C-
dc.contributor.authorPereira, Gislene-
dc.contributor.authorEdmondson, Ricky D-
dc.contributor.authorAllen, Terence D-
dc.contributor.authorLabib, Karim-
dc.date.accessioned2009-04-23T16:05:07Z-
dc.date.available2009-04-23T16:05:07Z-
dc.date.issued2008-04-
dc.identifier.citationInn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast. 2008, 10 (4):395-406 Nat. Cell Biol.en
dc.identifier.issn1476-4679-
dc.identifier.pmid18344988-
dc.identifier.doi10.1038/ncb1701-
dc.identifier.urihttp://hdl.handle.net/10541/66076-
dc.description.abstractBy rapidly depleting each of the essential budding yeast proteins of unknown function, we identified a novel factor that we call Inn1, which associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. We show that Inn1 has a C2 domain at the amino terminus of the protein that is required for ingression of the plasma membrane, whereas the remainder of the protein recruits Inn1 to the actomyosin ring. The lethal effects of deleting the INN1 gene can be suppressed by artificial fusion of the C2 domain to other components of the actomyosin ring, restoring membrane ingression on contraction of the actomyosin ring. Our data indicate that recruitment of the C2 domain of Inn1 to the contractile actomyosin ring is crucial for ingression of the plasma membrane during cytokinesis in budding yeast.en
dc.language.isoenen
dc.subject.meshActomyosin-
dc.subject.meshAmino Acid Sequence-
dc.subject.meshCell Cycle Proteins-
dc.subject.meshCell Membrane-
dc.subject.meshCytokinesis-
dc.subject.meshCytoskeletal Proteins-
dc.subject.meshCytoskeleton-
dc.subject.meshMitosis-
dc.subject.meshModels, Molecular-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshProtein Conformation-
dc.subject.meshProteomics-
dc.subject.meshRecombinant Fusion Proteins-
dc.subject.meshSaccharomyces Cerevisiae-
dc.subject.meshSaccharomyces Cerevisiae Proteins-
dc.subject.meshSequence Alignment-
dc.titleInn1 couples contraction of the actomyosin ring to membrane ingression during cytokinesis in budding yeast.en
dc.typeArticleen
dc.contributor.departmentCancer Research U.K., Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, M20 4BX, UK.en
dc.identifier.journalNature Cell Biologyen

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