2.50
Hdl Handle:
http://hdl.handle.net/10541/58733
Title:
Fission yeast MAP kinase Sty1 is recruited to stress-induced genes.
Authors:
Reiter, Wolfgang; Watt, Stephen; Dawson, Keren; Lawrence, Clare L; Bähler, Jürg; Jones, Nic; Wilkinson, Caroline R M
Abstract:
The stress-induced expression of many fission yeast genes is dependent upon the Sty1 mitogen-activated protein kinase (MAPK) and Atf1 transcription factor. Atf1 is phosphorylated by Sty1 yet this phosphorylation is not required for stress-induced gene expression, suggesting another mechanism exists whereby Sty1 activates transcription. Here we show that Sty1 associates with Atf1-dependent genes and is recruited to both their promoters and coding regions. This occurs in response to various stress conditions coincident with the kinetics of the activation of Sty1. Association with promoters is not a consequence of increased nuclear accumulation of Sty1 nor does it require the phosphorylation of Atf1. However, recruitment is completely abolished in a mutant lacking Sty1 kinase activity. Both Atf1 and its binding partner Pcr1 are required for association of Sty1 with Atf1-dependent promoters, suggesting that this heterodimer must be intact for optimal recruitment of the MAPK. However, many Atf1-dependent genes are still expressed in a pcr1Delta mutant but with significantly delayed kinetics, thus providing an explanation for the relatively mild stress sensitivity displayed by pcr1Delta. Consistent with this delay, Sty1 and Atf1 cannot be detected at these promoters in this condition, suggesting that their association with chromatin is weak or transient in the absence of Pcr1.
Affiliation:
Paterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, UK.
Citation:
Fission yeast MAP kinase Sty1 is recruited to stress-induced genes. 2008, 283 (15):9945-56 J. Biol. Chem.
Journal:
The Journal of Biological Chemistry
Issue Date:
11-Apr-2008
URI:
http://hdl.handle.net/10541/58733
DOI:
10.1074/jbc.M710428200
PubMed ID:
18252721
Type:
Article
Language:
en
ISSN:
0021-9258
Appears in Collections:
All Paterson Institute for Cancer Research; Cell Regulation

Full metadata record

DC FieldValue Language
dc.contributor.authorReiter, Wolfgang-
dc.contributor.authorWatt, Stephen-
dc.contributor.authorDawson, Keren-
dc.contributor.authorLawrence, Clare L-
dc.contributor.authorBähler, Jürg-
dc.contributor.authorJones, Nic-
dc.contributor.authorWilkinson, Caroline R M-
dc.date.accessioned2009-04-01T23:26:36Z-
dc.date.available2009-04-01T23:26:36Z-
dc.date.issued2008-04-11-
dc.identifier.citationFission yeast MAP kinase Sty1 is recruited to stress-induced genes. 2008, 283 (15):9945-56 J. Biol. Chem.en
dc.identifier.issn0021-9258-
dc.identifier.pmid18252721-
dc.identifier.doi10.1074/jbc.M710428200-
dc.identifier.urihttp://hdl.handle.net/10541/58733-
dc.description.abstractThe stress-induced expression of many fission yeast genes is dependent upon the Sty1 mitogen-activated protein kinase (MAPK) and Atf1 transcription factor. Atf1 is phosphorylated by Sty1 yet this phosphorylation is not required for stress-induced gene expression, suggesting another mechanism exists whereby Sty1 activates transcription. Here we show that Sty1 associates with Atf1-dependent genes and is recruited to both their promoters and coding regions. This occurs in response to various stress conditions coincident with the kinetics of the activation of Sty1. Association with promoters is not a consequence of increased nuclear accumulation of Sty1 nor does it require the phosphorylation of Atf1. However, recruitment is completely abolished in a mutant lacking Sty1 kinase activity. Both Atf1 and its binding partner Pcr1 are required for association of Sty1 with Atf1-dependent promoters, suggesting that this heterodimer must be intact for optimal recruitment of the MAPK. However, many Atf1-dependent genes are still expressed in a pcr1Delta mutant but with significantly delayed kinetics, thus providing an explanation for the relatively mild stress sensitivity displayed by pcr1Delta. Consistent with this delay, Sty1 and Atf1 cannot be detected at these promoters in this condition, suggesting that their association with chromatin is weak or transient in the absence of Pcr1.en
dc.language.isoenen
dc.subjectFission Yeasten
dc.subjectSty1en
dc.subject.meshActivating Transcription Factor 1-
dc.subject.meshActivating Transcription Factors-
dc.subject.meshActive Transport, Cell Nucleus-
dc.subject.meshCell Nucleus-
dc.subject.meshChromatin-
dc.subject.meshGene Deletion-
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshMitogen-Activated Protein Kinases-
dc.subject.meshOpen Reading Frames-
dc.subject.meshPhosphoproteins-
dc.subject.meshPhosphorylation-
dc.subject.meshPromoter Regions, Genetic-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces Pombe Proteins-
dc.titleFission yeast MAP kinase Sty1 is recruited to stress-induced genes.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, University of Manchester, Wilmslow Road, Manchester, UK.en
dc.identifier.journalThe Journal of Biological Chemistryen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.