The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure.

2.50
Hdl Handle:
http://hdl.handle.net/10541/58133
Title:
The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure.
Authors:
Wright, Daniel J; Munro, Ewen; Corbett, Mark; Bentley, Adam J; Fullwood, Nigel J; Murray, Stephen M; Price, Clive
Abstract:
Iqg1p is a component of the actomyosin contractile ring that is required for actin recruitment and septum deposition. Cells lacking Iqg1p function have an altered bud-neck structure and fail to form a functional actomyosin contractile ring resulting in a block to cytokinesis and septation. Here it is demonstrated that increased expression of the actin cytoskeleton associated protein Bsp1p bypasses the requirement for contractile ring function. This also correlates with reduced bud-neck width and remedial septum formation. Increased expression of this protein in a temperature-sensitive iqg1-1 background causes remedial septum formation at the bud neck that is reliant upon chitin synthase III activity and restores cell separation. The observed suppression correlates with a restoration of normal bud-neck structure. While Bsp1p is a component of the contractile ring, its recruitment to the bud neck is not required for the observed suppression. Loss of Bsp1p causes a brief delay in the redistribution of the actin cytoskeleton normally observed at the end of actin ring contraction. Compromise of Iqg1p function, in the absence of Bsp1p function, leads to a profound change in the distribution of actin and the pattern of cell growth accompanied by a failure to complete cytokinesis and cell separation.
Affiliation:
Biomedical Sciences Unit, Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom.
Citation:
The Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure. 2008, 178 (4):1903-14 Genetics
Journal:
Genetics
Issue Date:
Apr-2008
URI:
http://hdl.handle.net/10541/58133
DOI:
10.1534/genetics.107.082685
PubMed ID:
18430924
Type:
Article
Language:
en
ISSN:
0016-6731
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWright, Daniel J-
dc.contributor.authorMunro, Ewen-
dc.contributor.authorCorbett, Mark-
dc.contributor.authorBentley, Adam J-
dc.contributor.authorFullwood, Nigel J-
dc.contributor.authorMurray, Stephen M-
dc.contributor.authorPrice, Clive-
dc.date.accessioned2009-03-31T11:10:49Z-
dc.date.available2009-03-31T11:10:49Z-
dc.date.issued2008-04-
dc.identifier.citationThe Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure. 2008, 178 (4):1903-14 Geneticsen
dc.identifier.issn0016-6731-
dc.identifier.pmid18430924-
dc.identifier.doi10.1534/genetics.107.082685-
dc.identifier.urihttp://hdl.handle.net/10541/58133-
dc.description.abstractIqg1p is a component of the actomyosin contractile ring that is required for actin recruitment and septum deposition. Cells lacking Iqg1p function have an altered bud-neck structure and fail to form a functional actomyosin contractile ring resulting in a block to cytokinesis and septation. Here it is demonstrated that increased expression of the actin cytoskeleton associated protein Bsp1p bypasses the requirement for contractile ring function. This also correlates with reduced bud-neck width and remedial septum formation. Increased expression of this protein in a temperature-sensitive iqg1-1 background causes remedial septum formation at the bud neck that is reliant upon chitin synthase III activity and restores cell separation. The observed suppression correlates with a restoration of normal bud-neck structure. While Bsp1p is a component of the contractile ring, its recruitment to the bud neck is not required for the observed suppression. Loss of Bsp1p causes a brief delay in the redistribution of the actin cytoskeleton normally observed at the end of actin ring contraction. Compromise of Iqg1p function, in the absence of Bsp1p function, leads to a profound change in the distribution of actin and the pattern of cell growth accompanied by a failure to complete cytokinesis and cell separation.en
dc.language.isoenen
dc.subjectBud-Neck Structureen
dc.subjectCytokinesisen
dc.subjectGrowth Polarityen
dc.subject.meshActins-
dc.subject.meshActomyosin-
dc.subject.meshAdaptor Proteins, Vesicular Transport-
dc.subject.meshCell Wall-
dc.subject.meshChitin-
dc.subject.meshChitin Synthase-
dc.subject.meshCytoskeleton-
dc.subject.meshFluorescence Resonance Energy Transfer-
dc.subject.meshFungal Proteins-
dc.subject.meshMAP Kinase Signaling System-
dc.subject.meshMutation-
dc.subject.meshPhenotype-
dc.subject.meshProtein Binding-
dc.subject.meshProtein Transport-
dc.subject.meshSaccharomyces Cerevisiae-
dc.subject.meshSaccharomyces Cerevisiae Proteins-
dc.subject.meshSuppression, Genetic-
dc.subject.meshTemperature-
dc.subject.meshras GTPase-Activating Proteins-
dc.titleThe Saccharomyces cerevisiae actin cytoskeletal component Bsp1p has an auxiliary role in actomyosin ring function and in the maintenance of bud-neck structure.en
dc.typeArticleen
dc.contributor.departmentBiomedical Sciences Unit, Biological Sciences, Lancaster University, Lancaster LA1 4YQ, United Kingdom.en
dc.identifier.journalGeneticsen

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