Multiple pathways differentially regulate global oxidative stress responses in fission yeast.

2.50
Hdl Handle:
http://hdl.handle.net/10541/56017
Title:
Multiple pathways differentially regulate global oxidative stress responses in fission yeast.
Authors:
Chen, Dongrong; Wilkinson, Caroline R M; Watt, Stephen; Penkett, Christopher J; Toone, W Mark; Jones, Nic; Bähler, Jürg
Abstract:
Cellular protection against oxidative damage is relevant to ageing and numerous diseases. We analyzed the diversity of genome-wide gene expression programs and their regulation in response to various types and doses of oxidants in Schizosaccharomyces pombe. A small core gene set, regulated by the AP-1-like factor Pap1p and the two-component regulator Prr1p, was universally induced irrespective of oxidant and dose. Strong oxidative stresses led to a much larger transcriptional response. The mitogen-activated protein kinase (MAPK) Sty1p and the bZIP factor Atf1p were critical for the response to hydrogen peroxide. A newly identified zinc-finger protein, Hsr1p, is uniquely regulated by all three major regulatory systems (Sty1p-Atf1p, Pap1p, and Prr1p) and in turn globally supports gene expression in response to hydrogen peroxide. Although the overall transcriptional responses to hydrogen peroxide and t-butylhydroperoxide were similar, to our surprise, Sty1p and Atf1p were less critical for the response to the latter. Instead, another MAPK, Pmk1p, was involved in surviving this stress, although Pmk1p played only a minor role in regulating the transcriptional response. These data reveal a considerable plasticity and differential control of regulatory pathways in distinct oxidative stress conditions, providing both specificity and backup for protection from oxidative damage.
Affiliation:
Cancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.
Citation:
Multiple pathways differentially regulate global oxidative stress responses in fission yeast. 2008, 19 (1):308-17 Mol. Biol. Cell
Journal:
Molecular Biology of the Cell
Issue Date:
Jan-2008
URI:
http://hdl.handle.net/10541/56017
DOI:
10.1091/mbc.E07-08-0735
PubMed ID:
18003976
Type:
Article
Language:
en
ISSN:
1939-4586
Appears in Collections:
All Paterson Institute for Cancer Research; Cell Regulation

Full metadata record

DC FieldValue Language
dc.contributor.authorChen, Dongrong-
dc.contributor.authorWilkinson, Caroline R M-
dc.contributor.authorWatt, Stephen-
dc.contributor.authorPenkett, Christopher J-
dc.contributor.authorToone, W Mark-
dc.contributor.authorJones, Nic-
dc.contributor.authorBähler, Jürg-
dc.date.accessioned2009-03-17T17:01:28Z-
dc.date.available2009-03-17T17:01:28Z-
dc.date.issued2008-01-
dc.identifier.citationMultiple pathways differentially regulate global oxidative stress responses in fission yeast. 2008, 19 (1):308-17 Mol. Biol. Cellen
dc.identifier.issn1939-4586-
dc.identifier.pmid18003976-
dc.identifier.doi10.1091/mbc.E07-08-0735-
dc.identifier.urihttp://hdl.handle.net/10541/56017-
dc.description.abstractCellular protection against oxidative damage is relevant to ageing and numerous diseases. We analyzed the diversity of genome-wide gene expression programs and their regulation in response to various types and doses of oxidants in Schizosaccharomyces pombe. A small core gene set, regulated by the AP-1-like factor Pap1p and the two-component regulator Prr1p, was universally induced irrespective of oxidant and dose. Strong oxidative stresses led to a much larger transcriptional response. The mitogen-activated protein kinase (MAPK) Sty1p and the bZIP factor Atf1p were critical for the response to hydrogen peroxide. A newly identified zinc-finger protein, Hsr1p, is uniquely regulated by all three major regulatory systems (Sty1p-Atf1p, Pap1p, and Prr1p) and in turn globally supports gene expression in response to hydrogen peroxide. Although the overall transcriptional responses to hydrogen peroxide and t-butylhydroperoxide were similar, to our surprise, Sty1p and Atf1p were less critical for the response to the latter. Instead, another MAPK, Pmk1p, was involved in surviving this stress, although Pmk1p played only a minor role in regulating the transcriptional response. These data reveal a considerable plasticity and differential control of regulatory pathways in distinct oxidative stress conditions, providing both specificity and backup for protection from oxidative damage.en
dc.language.isoenen
dc.subjectOxiative Stress Responseen
dc.subjectOxidative Damageen
dc.subjectSchizosaccharomyces Pombeen
dc.subject.meshGene Expression Regulation, Fungal-
dc.subject.meshGenes, Fungal-
dc.subject.meshHydrogen Peroxide-
dc.subject.meshMitogen-Activated Protein Kinase Kinases-
dc.subject.meshOxidants-
dc.subject.meshOxidative Stress-
dc.subject.meshSchizosaccharomyces-
dc.subject.meshSchizosaccharomyces Pombe Proteins-
dc.subject.meshSignal Transduction-
dc.subject.meshVitamin K 3-
dc.subject.meshtert-Butylhydroperoxide-
dc.titleMultiple pathways differentially regulate global oxidative stress responses in fission yeast.en
dc.typeArticleen
dc.contributor.departmentCancer Research UK Fission Yeast Functional Genomics Group, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1HH, United Kingdom.en
dc.identifier.journalMolecular Biology of the Cellen

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