QuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma.

2.50
Hdl Handle:
http://hdl.handle.net/10541/558726
Title:
QuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma.
Authors:
Hall, J; Usher, S; Byers, R; Higgins, R; Memon, D; Radford, John A ( 0000-0001-7898-2786 ) ; Linton, Kim M ( 0000-0002-3294-1548 )
Abstract:
Emerging therapies targeting the molecularly distinct GCB and non-GCB/ABC subtypes of diffuse large B-cell lymphoma (DLBCL) have created the need to develop an accurate subtyping assay for routine use. We investigated the potential of QuantiGene Plex (QGP)-branched DNA signal amplification assay-for DLBCL subtyping. We performed in silico analysis of public DLBCL datasets to develop and validate a naïve Bayes classifier, and migrated the resulting 21-gene classifier to QGP and real-time quantitative PCR (qPCR) assays. Forty DLBCL formalin-fixed, paraffin-embedded tumors of known subtype (20 per subtype by gene expression profiling of paired fresh-frozen tissues) were reclassified, and results for QGP (on 38/40 for 21/21 targets) and qPCR (on 40/40 samples for 19/21 targets) compared for recapitulation of microarray data and classification accuracy. The 21-gene bayesian classifier achieved mean area under the curve values >0.9 on independent validation. QGP showed a higher correlation with microarray data (mean R(2) = 0.66 ± 0.05 versus 0.34 ± 0.07; P < 0.0001) and classification accuracy (92.1% versus 78.9%). The proportion of validated targets was also higher for QGP (85.7% versus 47.4%). The QGP protocol was rapid and simple to perform, at a cost similar to qPCR. These promising preliminary results strongly support ongoing work to develop a QGP companion diagnostic assay for DLBCL subtyping.
Affiliation:
Lymphoma Translational Research Group, Manchester Academic Health Science Centre, The University of Manchester, Manchester, United Kingdom
Citation:
QuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma. 2015, 17 (4):402-11 J Mol Diagn
Journal:
The Journal of Molecular Diagnostics
Issue Date:
Jul-2015
URI:
http://hdl.handle.net/10541/558726
DOI:
10.1016/j.jmoldx.2015.03.010
PubMed ID:
25982535
Type:
Article
Language:
en
ISSN:
1943-7811
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorHall, Jen
dc.contributor.authorUsher, Sen
dc.contributor.authorByers, Ren
dc.contributor.authorHiggins, Ren
dc.contributor.authorMemon, Den
dc.contributor.authorRadford, John Aen
dc.contributor.authorLinton, Kim Men
dc.date.accessioned2015-07-01T07:51:23Zen
dc.date.available2015-07-01T07:51:23Zen
dc.date.issued2015-07en
dc.identifier.citationQuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma. 2015, 17 (4):402-11 J Mol Diagnen
dc.identifier.issn1943-7811en
dc.identifier.pmid25982535en
dc.identifier.doi10.1016/j.jmoldx.2015.03.010en
dc.identifier.urihttp://hdl.handle.net/10541/558726en
dc.description.abstractEmerging therapies targeting the molecularly distinct GCB and non-GCB/ABC subtypes of diffuse large B-cell lymphoma (DLBCL) have created the need to develop an accurate subtyping assay for routine use. We investigated the potential of QuantiGene Plex (QGP)-branched DNA signal amplification assay-for DLBCL subtyping. We performed in silico analysis of public DLBCL datasets to develop and validate a naïve Bayes classifier, and migrated the resulting 21-gene classifier to QGP and real-time quantitative PCR (qPCR) assays. Forty DLBCL formalin-fixed, paraffin-embedded tumors of known subtype (20 per subtype by gene expression profiling of paired fresh-frozen tissues) were reclassified, and results for QGP (on 38/40 for 21/21 targets) and qPCR (on 40/40 samples for 19/21 targets) compared for recapitulation of microarray data and classification accuracy. The 21-gene bayesian classifier achieved mean area under the curve values >0.9 on independent validation. QGP showed a higher correlation with microarray data (mean R(2) = 0.66 ± 0.05 versus 0.34 ± 0.07; P < 0.0001) and classification accuracy (92.1% versus 78.9%). The proportion of validated targets was also higher for QGP (85.7% versus 47.4%). The QGP protocol was rapid and simple to perform, at a cost similar to qPCR. These promising preliminary results strongly support ongoing work to develop a QGP companion diagnostic assay for DLBCL subtyping.en
dc.language.isoenen
dc.rightsArchived with thanks to The Journal of molecular diagnostics : JMDen
dc.titleQuantiGene Plex Represents a Promising Diagnostic Tool for Cell-of-Origin Subtyping of Diffuse Large B-Cell Lymphoma.en
dc.typeArticleen
dc.contributor.departmentLymphoma Translational Research Group, Manchester Academic Health Science Centre, The University of Manchester, Manchester, United Kingdomen
dc.identifier.journalThe Journal of Molecular Diagnosticsen
dc.description.collectionLymphoma Research Teamen

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