2.50
Hdl Handle:
http://hdl.handle.net/10541/53433
Title:
THOC5 couples M-CSF receptor signaling to transcription factor expression.
Authors:
Carney, Louise; Pierce, Andrew; Rijnen, Marco; Gonzalez Sanchez, M Belen; Hamzah, Hajja G; Zhang, Liqun; Tamura, Teruko; Whetton, Anthony D
Abstract:
THOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPbeta. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPbeta, C/EBPalpha, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP(3). Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation.
Affiliation:
Stem Cell and Leukaemia Proteomics laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK.
Citation:
THOC5 couples M-CSF receptor signaling to transcription factor expression. 2009, 21 (2):309-16 Cell. Signal.
Journal:
Cellular Signalling
Issue Date:
Feb-2009
URI:
http://hdl.handle.net/10541/53433
DOI:
10.1016/j.cellsig.2008.10.018
PubMed ID:
19015024
Type:
Article
Language:
en
ISSN:
1873-3913
Appears in Collections:
All Paterson Institute for Cancer Research; School of Cancer and Imaging Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorCarney, Louise-
dc.contributor.authorPierce, Andrew-
dc.contributor.authorRijnen, Marco-
dc.contributor.authorGonzalez Sanchez, M Belen-
dc.contributor.authorHamzah, Hajja G-
dc.contributor.authorZhang, Liqun-
dc.contributor.authorTamura, Teruko-
dc.contributor.authorWhetton, Anthony D-
dc.date.accessioned2009-03-09T15:58:44Z-
dc.date.available2009-03-09T15:58:44Z-
dc.date.issued2009-02-
dc.identifier.citationTHOC5 couples M-CSF receptor signaling to transcription factor expression. 2009, 21 (2):309-16 Cell. Signal.en
dc.identifier.issn1873-3913-
dc.identifier.pmid19015024-
dc.identifier.doi10.1016/j.cellsig.2008.10.018-
dc.identifier.urihttp://hdl.handle.net/10541/53433-
dc.description.abstractTHOC5 is a nuclear/cytoplasmic protein member of the spliceosome complex which potentiates C/EBP expression in adipocyte differentiation. As C/EBP family members are important regulators of myelopoiesis and THOC5 is highly expressed in neutrophil/macrophage progenitor cells we assessed the role of THOC5 in cytokine-stimulated monocytic development. M-CSF stimulated maturation of the NFS60 cell line was associated with enhanced THOC5 expression and phosphorylation. THOC5 was also shown to form a complex with C/EBPbeta. Ectopic expression of THOC5 mimicked M-CSF mediated cell maturation and enhanced protein expression of the myeloid transcription factors C/EBPbeta, C/EBPalpha, Pu-1 and also GAB2 (a PI-3 Kinase and macrophage development regulator). Increased THOC5 expression also mimicked M-CSF stimulated increases in the lipid second messenger PtdInsP(3). Inhibition of THOC5-induced increases in PtdInsP(3) levels abrogated the elevated levels of C/EBPbeta. Thus THOC5 expression can potentiate receptor signalling to transcription factor expression and monocyte differentiation.en
dc.language.isoenen
dc.subjectTHOC5en
dc.subjectC/EBPen
dc.subjectTranscriptionen
dc.subjectCell Signallingen
dc.subjectM-CSF Receptor Signalingen
dc.subject.mesh1-Phosphatidylinositol 3-Kinase-
dc.subject.meshAnimals-
dc.subject.meshCCAAT-Enhancer-Binding Protein-beta-
dc.subject.meshCell Line-
dc.subject.meshHumans-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMice-
dc.subject.meshMonocytes-
dc.subject.meshNuclear Proteins-
dc.subject.meshPhosphorylation-
dc.subject.meshReceptor, Macrophage Colony-Stimulating Factor-
dc.subject.meshSignal Transduction-
dc.subject.meshTranscription Factors-
dc.subject.meshTransfection-
dc.titleTHOC5 couples M-CSF receptor signaling to transcription factor expression.en
dc.typeArticleen
dc.contributor.departmentStem Cell and Leukaemia Proteomics laboratory, Faculty of Medical and Human Sciences, University of Manchester, Christie Hospital, Wilmslow Road, Manchester M20 9BX, UK.en
dc.identifier.journalCellular Signallingen

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