Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).

2.50
Hdl Handle:
http://hdl.handle.net/10541/53393
Title:
Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).
Authors:
El Bounkari, Omar; Guria, Anuja; Klebba-Faerber, Sabine; Claussen, Maike; Pieler, Tomas; Griffiths, John R; Whetton, Anthony D; Koch, Alexandra; Tamura, Teruko
Abstract:
THOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.
Affiliation:
Institut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Hannover, Germany.
Citation:
Nuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP). 2009, 583 (1):13-8 FEBS Lett.
Journal:
FEBS Letters
Issue Date:
5-Jan-2009
URI:
http://hdl.handle.net/10541/53393
DOI:
10.1016/j.febslet.2008.11.024
PubMed ID:
19059247
Type:
Article
Language:
en
ISSN:
1873-3468
Appears in Collections:
All Paterson Institute for Cancer Research; School of Cancer and Imaging Sciences

Full metadata record

DC FieldValue Language
dc.contributor.authorEl Bounkari, Omar-
dc.contributor.authorGuria, Anuja-
dc.contributor.authorKlebba-Faerber, Sabine-
dc.contributor.authorClaussen, Maike-
dc.contributor.authorPieler, Tomas-
dc.contributor.authorGriffiths, John R-
dc.contributor.authorWhetton, Anthony D-
dc.contributor.authorKoch, Alexandra-
dc.contributor.authorTamura, Teruko-
dc.date.accessioned2009-03-09T15:40:49Z-
dc.date.available2009-03-09T15:40:49Z-
dc.date.issued2009-01-05-
dc.identifier.citationNuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP). 2009, 583 (1):13-8 FEBS Lett.en
dc.identifier.issn1873-3468-
dc.identifier.pmid19059247-
dc.identifier.doi10.1016/j.febslet.2008.11.024-
dc.identifier.urihttp://hdl.handle.net/10541/53393-
dc.description.abstractTHOC7 and Fms-interacting protein (FMIP) are members of the THO complex that associate with the mRNA export apparatus. FMIP is a nucleocytoplasmic shuttling protein with a nuclear localization signal (NLS), whereas THOC7 does not contain a typical NLS motif. We show here that THOC7 (50-137, amino acid numbers) binds to the N-terminal portion (1-199) of FMIP directly. FMIP is detected mainly in the nucleus. In the absence of exogenous FMIP, THOC7 resides mainly in the cytoplasm, while in the presence of FMIP, THOC7 is transported into the nucleus with FMIP. Furthermore, THOC7 lacking the FMIP binding site does not co-localize with FMIP, indicating that THOC7/FMIP interaction is required for nuclear localization of THOC7.en
dc.language.isoenen
dc.subjectmRNAen
dc.subjectTHOC7en
dc.subjectFMIPen
dc.subjectFms Tyrosine Kinase Interacting Proteinen
dc.titleNuclear localization of the pre-mRNA associating protein THOC7 depends upon its direct interaction with Fms tyrosine kinase interacting protein (FMIP).en
dc.typeArticleen
dc.contributor.departmentInstitut fuer Biochemie, OE4310 Medizinische Hochschule Hannover, Hannover, Germany.en
dc.identifier.journalFEBS Lettersen
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