Functional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS).

2.50
Hdl Handle:
http://hdl.handle.net/10541/109882
Title:
Functional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS).
Authors:
Coutinho, Lucia H; Geary, C; Chang, James; Harrison, Christine J; Testa, Nydia G
Abstract:
Long-term bone marrow culture (LTBMC) was used to investigate the proliferative behaviour of marrow cells from a spectrum of cases of the myelodysplastic syndrome (MDS), and the results compared with those obtained in the conventional short-term clonal assay. Two broad patterns of growth were revealed in LTBMC. In one group the incidence of haemopoietic progenitor cells steadily declined to abnormally low levels at 4 weeks, while in a second group they were maintained near normal levels for periods of up to 7 weeks. These growth patterns, which were not predictable from clonogenic assays on the marrow cells prior to LTBMC, or from the morphology of the bone marrow, may reflect the stage of evolution of the disease. Further studies of clonality are required to establish whether or not patients exhibiting the second pattern have a potentiality to harbour residual normal haemopoiesis. LTBMC was also used to study the function of MDS marrow stroma in terms of its ability to sustain the growth of normal haemopoietic progenitor cells. Although the phenotype of the cultured adherent cell layer, obtained from some patients, was atypical, no consistent functional defect of MDS stroma could be identified by studying the level of haemopoiesis reached by normal cells seeded into MDS stroma.
Affiliation:
Experimental Haematology, Christie Hospital and Holt Radium Institute, Manchester.
Citation:
Functional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS). 1990, 75 (1):16-25 Br. J. Haematol.
Journal:
British Journal of Haematology
Issue Date:
May-1990
URI:
http://hdl.handle.net/10541/109882
DOI:
10.1111/j.1365-2141.1990.tb02611.x
PubMed ID:
2375919
Type:
Article
Language:
en
ISSN:
0007-1048
Appears in Collections:
All Christie Publications

Full metadata record

DC FieldValue Language
dc.contributor.authorCoutinho, Lucia Hen
dc.contributor.authorGeary, Cen
dc.contributor.authorChang, Jamesen
dc.contributor.authorHarrison, Christine Jen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-08-18T15:08:56Z-
dc.date.available2010-08-18T15:08:56Z-
dc.date.issued1990-05-
dc.identifier.citationFunctional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS). 1990, 75 (1):16-25 Br. J. Haematol.en
dc.identifier.issn0007-1048-
dc.identifier.pmid2375919-
dc.identifier.doi10.1111/j.1365-2141.1990.tb02611.x-
dc.identifier.urihttp://hdl.handle.net/10541/109882-
dc.description.abstractLong-term bone marrow culture (LTBMC) was used to investigate the proliferative behaviour of marrow cells from a spectrum of cases of the myelodysplastic syndrome (MDS), and the results compared with those obtained in the conventional short-term clonal assay. Two broad patterns of growth were revealed in LTBMC. In one group the incidence of haemopoietic progenitor cells steadily declined to abnormally low levels at 4 weeks, while in a second group they were maintained near normal levels for periods of up to 7 weeks. These growth patterns, which were not predictable from clonogenic assays on the marrow cells prior to LTBMC, or from the morphology of the bone marrow, may reflect the stage of evolution of the disease. Further studies of clonality are required to establish whether or not patients exhibiting the second pattern have a potentiality to harbour residual normal haemopoiesis. LTBMC was also used to study the function of MDS marrow stroma in terms of its ability to sustain the growth of normal haemopoietic progenitor cells. Although the phenotype of the cultured adherent cell layer, obtained from some patients, was atypical, no consistent functional defect of MDS stroma could be identified by studying the level of haemopoiesis reached by normal cells seeded into MDS stroma.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAged, 80 and over-
dc.subject.meshBone Marrow-
dc.subject.meshBone Marrow Cells-
dc.subject.meshCell Adhesion-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Division-
dc.subject.meshChild-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshFemale-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMyelodysplastic Syndromes-
dc.subject.meshTime Factors-
dc.titleFunctional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS).en
dc.typeArticleen
dc.contributor.departmentExperimental Haematology, Christie Hospital and Holt Radium Institute, Manchester.en
dc.identifier.journalBritish Journal of Haematologyen

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