Radiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109858
Title:
Radiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity.
Authors:
Davidson, Susan E; West, Catharine M L; Roberts, Stephen A; Hendry, Jolyon H; Hunter, Robin D
Abstract:
Biopsies from 89 patients with cervical carcinoma were studied using a clonogenic assay to obtain values for the surviving fraction at 2 Gy (SF2). Heterogeneity in intrinsic radiosensitivity was investigated by independently processing multiple biopsies from 18 tumors. No significant differences between intra-tumour SF2 values were demonstrated (p = 0.30). The results have shown that intra-tumour heterogeneity is not a limitation to radiosensitivity testing using the Courtenay-Mills assay. A wide range of values (0.13-0.97) for SF2 was obtained with a mean value of 0.47 +/- 0.18 (+/- 1 S.D., CV = 38%) for 52 squamous cell carcinomas and 0.59 +/- 0.27 for four adenocarcinomas. There were statistically significant differences between the individual tumours (p less than 0.001). From the analysis-of-variance of all the SF2 results it appears to be the surviving fractions below about 0.40 and those above about 0.7 which show significant differences in radiosensitivity between pairs of tumours (p = 0.05). Also 36% of the values of SF2 show significant differences from the mean SF2 of all tumours. The storage of tumour cell suspensions in liquid nitrogen improved the colony-forming efficiency (CFE) but it did not alter the radiosensitivity.
Affiliation:
Department of Radiobiology, Paterson Institute for Cancer Research, Manchester, U.K.
Citation:
Radiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity. 1990, 18 (4):349-56 Radiother Oncol
Journal:
Radiotherapy and Oncology
Issue Date:
Aug-1990
URI:
http://hdl.handle.net/10541/109858
DOI:
10.1016/0167-8140(90)90115-D
PubMed ID:
2244021
Type:
Article
Language:
en
ISSN:
0167-8140
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorDavidson, Susan Een
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorRoberts, Stephen Aen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorHunter, Robin Den
dc.date.accessioned2010-08-18T15:19:37Z-
dc.date.available2010-08-18T15:19:37Z-
dc.date.issued1990-08-
dc.identifier.citationRadiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity. 1990, 18 (4):349-56 Radiother Oncolen
dc.identifier.issn0167-8140-
dc.identifier.pmid2244021-
dc.identifier.doi10.1016/0167-8140(90)90115-D-
dc.identifier.urihttp://hdl.handle.net/10541/109858-
dc.description.abstractBiopsies from 89 patients with cervical carcinoma were studied using a clonogenic assay to obtain values for the surviving fraction at 2 Gy (SF2). Heterogeneity in intrinsic radiosensitivity was investigated by independently processing multiple biopsies from 18 tumors. No significant differences between intra-tumour SF2 values were demonstrated (p = 0.30). The results have shown that intra-tumour heterogeneity is not a limitation to radiosensitivity testing using the Courtenay-Mills assay. A wide range of values (0.13-0.97) for SF2 was obtained with a mean value of 0.47 +/- 0.18 (+/- 1 S.D., CV = 38%) for 52 squamous cell carcinomas and 0.59 +/- 0.27 for four adenocarcinomas. There were statistically significant differences between the individual tumours (p less than 0.001). From the analysis-of-variance of all the SF2 results it appears to be the surviving fractions below about 0.40 and those above about 0.7 which show significant differences in radiosensitivity between pairs of tumours (p = 0.05). Also 36% of the values of SF2 show significant differences from the mean SF2 of all tumours. The storage of tumour cell suspensions in liquid nitrogen improved the colony-forming efficiency (CFE) but it did not alter the radiosensitivity.en
dc.language.isoenen
dc.subjectTumour Stem Cell Assayen
dc.subjectUterine Cervical Canceren
dc.subject.meshAnalysis of Variance-
dc.subject.meshCarcinoma-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshRadiation Tolerance-
dc.subject.meshTumor Stem Cell Assay-
dc.subject.meshUterine Cervical Neoplasms-
dc.titleRadiosensitivity testing of primary cervical carcinoma: evaluation of intra- and inter-tumour heterogeneity.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiobiology, Paterson Institute for Cancer Research, Manchester, U.K.en
dc.identifier.journalRadiotherapy and Oncologyen

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