Contrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109845
Title:
Contrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones.
Authors:
Butler, John; Dzielendziak, A; Lea, J S; Ward, Timothy H; Hoey, Brigid M
Abstract:
The mechanisms of cytotoxicity of the antitumour diaziridinylbenzoquinones, AZQ and BZQ, have been investigated. HPLC analysis has been used to study the products as well as the rate of decomposition of acid-assisted ring-opening in aqueous medium as a function of pH. Microconcentrators with a molecular weight cutoff of 30 kDa were utilised to study the covalent binding of both compounds to calf thymus DNA. Radical production of both compounds in K562 cell incubations was followed using ESR and their uptake into K562 cells was monitored using radiolabelled compounds. The results show that these two diaziridinylbenzoquinones, although very similar in structure, have diverse mechanisms of cytotoxicity. The implications of these findings are discussed in the light of antitumor action.
Affiliation:
Department of Biophysical Chemistry, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.
Citation:
Contrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones. 1990, 8 (4-6):231-9 Free Radic. Res. Commun.
Journal:
Free Radical Research Communications
Issue Date:
1990
URI:
http://hdl.handle.net/10541/109845
DOI:
10.3109/10715769009053356
PubMed ID:
2162311
Type:
Article
Language:
en
ISSN:
8755-0199
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorButler, Johnen
dc.contributor.authorDzielendziak, Aen
dc.contributor.authorLea, J Sen
dc.contributor.authorWard, Timothy Hen
dc.contributor.authorHoey, Brigid Men
dc.date.accessioned2010-08-18T12:56:44Z-
dc.date.available2010-08-18T12:56:44Z-
dc.date.issued1990-
dc.identifier.citationContrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones. 1990, 8 (4-6):231-9 Free Radic. Res. Commun.en
dc.identifier.issn8755-0199-
dc.identifier.pmid2162311-
dc.identifier.doi10.3109/10715769009053356-
dc.identifier.urihttp://hdl.handle.net/10541/109845-
dc.description.abstractThe mechanisms of cytotoxicity of the antitumour diaziridinylbenzoquinones, AZQ and BZQ, have been investigated. HPLC analysis has been used to study the products as well as the rate of decomposition of acid-assisted ring-opening in aqueous medium as a function of pH. Microconcentrators with a molecular weight cutoff of 30 kDa were utilised to study the covalent binding of both compounds to calf thymus DNA. Radical production of both compounds in K562 cell incubations was followed using ESR and their uptake into K562 cells was monitored using radiolabelled compounds. The results show that these two diaziridinylbenzoquinones, although very similar in structure, have diverse mechanisms of cytotoxicity. The implications of these findings are discussed in the light of antitumor action.en
dc.language.isoenen
dc.subjectAnticancerous Agentsen
dc.subject.meshAlkylating Agents-
dc.subject.meshAnimals-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshAziridines-
dc.subject.meshBenzoquinones-
dc.subject.meshCell Survival-
dc.subject.meshChromatography, High Pressure Liquid-
dc.subject.meshDNA-
dc.subject.meshDrug Stability-
dc.subject.meshElectron Spin Resonance Spectroscopy-
dc.subject.meshFree Radicals-
dc.subject.meshHumans-
dc.subject.meshHydrogen-Ion Concentration-
dc.subject.meshKinetics-
dc.subject.meshOxidation-Reduction-
dc.titleContrasting cytotoxic mechanisms of similar antitumour diaziridinylbenzoquinones.en
dc.typeArticleen
dc.contributor.departmentDepartment of Biophysical Chemistry, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, U.K.en
dc.identifier.journalFree Radical Research Communicationsen
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