The hematopoietic defect in aplastic anemia assessed by long-term marrow culture.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109835
Title:
The hematopoietic defect in aplastic anemia assessed by long-term marrow culture.
Authors:
Marsh, Judith C W; Chang, James; Testa, Nydia G; Hows, J M; Dexter, T Michael
Abstract:
Thirty-two patients with aplastic anemia (AA) have been studied using the long-term bone marrow culture (LTBMC) system. Of these patients, 26 had been treated with immunosuppressive therapy including antilymphocyte globulin (ALG) with or without androgens or high-dose methyl prednisolone. The remaining six patients either required no treatment or were studied before therapy was begun. Thirty-one of 32 patients (96%) had defective hematopoiesis in LTBMC with little or no evidence for the generation of primitive progenitor cells. The only exception was a patient with spontaneous recovery of aplasia in whom the defect was less marked. Crossover LTBMC experiments were performed in 23 cases by inoculating (1) patient marrow hematopoietic cells that had been depleted of adherent cells onto preformed, irradiated, normal stromas to assess the proliferative capacity of the hematopoietic cells, and (2) normal marrow hematopoietic cells that were depleted of adherent cells onto preformed, irradiated stromas from patients with AA to assess stromal function. Results of these experiments demonstrated a hematopoietic defect in all patients that was independent of the degree of hematologic recovery after ALG therapy. Only one patient had a probable stromal defect and this coexisted with a defect in the regenerative capacity of hematopoietic cells. We conclude that LTBMC is a sensitive method for detecting and defining the hematopoietic failure in AA. We suggest that the defective hematopoiesis present in all patients studied may be important in the pathogenesis of clonal evolution in AA.
Affiliation:
Department of Experimental Hematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.
Citation:
The hematopoietic defect in aplastic anemia assessed by long-term marrow culture. 1990, 76 (9):1748-57 Blood
Journal:
Blood
Issue Date:
1-Nov-1990
URI:
http://hdl.handle.net/10541/109835
PubMed ID:
2224124
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMarsh, Judith C Wen
dc.contributor.authorChang, Jamesen
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorHows, J Men
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-08-18T10:28:20Z-
dc.date.available2010-08-18T10:28:20Z-
dc.date.issued1990-11-01-
dc.identifier.citationThe hematopoietic defect in aplastic anemia assessed by long-term marrow culture. 1990, 76 (9):1748-57 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid2224124-
dc.identifier.urihttp://hdl.handle.net/10541/109835-
dc.description.abstractThirty-two patients with aplastic anemia (AA) have been studied using the long-term bone marrow culture (LTBMC) system. Of these patients, 26 had been treated with immunosuppressive therapy including antilymphocyte globulin (ALG) with or without androgens or high-dose methyl prednisolone. The remaining six patients either required no treatment or were studied before therapy was begun. Thirty-one of 32 patients (96%) had defective hematopoiesis in LTBMC with little or no evidence for the generation of primitive progenitor cells. The only exception was a patient with spontaneous recovery of aplasia in whom the defect was less marked. Crossover LTBMC experiments were performed in 23 cases by inoculating (1) patient marrow hematopoietic cells that had been depleted of adherent cells onto preformed, irradiated, normal stromas to assess the proliferative capacity of the hematopoietic cells, and (2) normal marrow hematopoietic cells that were depleted of adherent cells onto preformed, irradiated stromas from patients with AA to assess stromal function. Results of these experiments demonstrated a hematopoietic defect in all patients that was independent of the degree of hematologic recovery after ALG therapy. Only one patient had a probable stromal defect and this coexisted with a defect in the regenerative capacity of hematopoietic cells. We conclude that LTBMC is a sensitive method for detecting and defining the hematopoietic failure in AA. We suggest that the defective hematopoiesis present in all patients studied may be important in the pathogenesis of clonal evolution in AA.en
dc.language.isoenen
dc.subjectAplastic Anaemiaen
dc.subjectHaematopoiesisen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAdolescent-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshAnemia, Aplastic-
dc.subject.meshBone Marrow-
dc.subject.meshCell Adhesion-
dc.subject.meshCells, Cultured-
dc.subject.meshFemale-
dc.subject.meshHematopoiesis-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshTime Factors-
dc.titleThe hematopoietic defect in aplastic anemia assessed by long-term marrow culture.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Hematology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, England.en
dc.identifier.journalBlooden

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