Radiosensitivity increases with differentiation status of murine hemopoietic progenitor cells selected using enriched marrow subpopulations and recombinant growth factors.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109829
Title:
Radiosensitivity increases with differentiation status of murine hemopoietic progenitor cells selected using enriched marrow subpopulations and recombinant growth factors.
Authors:
Baird, M C; Hendry, Jolyon H; Testa, Nydia G
Abstract:
The radiosensitivity of populations of colony-forming cells (CFC) in murine bone marrow was investigated using different recombinant colony-stimulating factors (CSFs; murine IL-3 and granulocyte-macrophage CSF and human granulocyte CSF), or purified murine macrophage CSF. With unfractionated normal bone marrow the CFC increased in radiosensitivity as they progressed through the granulocyte lineage. The D0 values ranged from 129 +/- 12 cGy for CFC stimulated with GM-CSF down to 42 +/- 2 cGy after stimulation with G-CSF. IL-3 stimulated a CFC population which gave the only survival curve with a shoulder (n = 1.9 +/- 0.3). With semipurified populations of primitive or bipotential CFC, D0 values were generally lower with respect to the equivalent values for unpurified bone marrow (range 62 +/- 7 cGy to 135 +/- 7 cGy). Changes in cluster/colony ratio and colony morphology together possibly with products of accessory cells influence the interpretation of the radiosensitivity parameters.
Affiliation:
Department of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.
Citation:
Radiosensitivity increases with differentiation status of murine hemopoietic progenitor cells selected using enriched marrow subpopulations and recombinant growth factors. 1990, 123 (3):292-8 Radiat. Res.
Journal:
Radiation Research
Issue Date:
Sep-1990
URI:
http://hdl.handle.net/10541/109829
PubMed ID:
2217726
Type:
Article
Language:
en
ISSN:
0033-7587
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBaird, M Cen
dc.contributor.authorHendry, Jolyon Hen
dc.contributor.authorTesta, Nydia Gen
dc.date.accessioned2010-08-18T12:21:59Z-
dc.date.available2010-08-18T12:21:59Z-
dc.date.issued1990-09-
dc.identifier.citationRadiosensitivity increases with differentiation status of murine hemopoietic progenitor cells selected using enriched marrow subpopulations and recombinant growth factors. 1990, 123 (3):292-8 Radiat. Res.en
dc.identifier.issn0033-7587-
dc.identifier.pmid2217726-
dc.identifier.urihttp://hdl.handle.net/10541/109829-
dc.description.abstractThe radiosensitivity of populations of colony-forming cells (CFC) in murine bone marrow was investigated using different recombinant colony-stimulating factors (CSFs; murine IL-3 and granulocyte-macrophage CSF and human granulocyte CSF), or purified murine macrophage CSF. With unfractionated normal bone marrow the CFC increased in radiosensitivity as they progressed through the granulocyte lineage. The D0 values ranged from 129 +/- 12 cGy for CFC stimulated with GM-CSF down to 42 +/- 2 cGy after stimulation with G-CSF. IL-3 stimulated a CFC population which gave the only survival curve with a shoulder (n = 1.9 +/- 0.3). With semipurified populations of primitive or bipotential CFC, D0 values were generally lower with respect to the equivalent values for unpurified bone marrow (range 62 +/- 7 cGy to 135 +/- 7 cGy). Changes in cluster/colony ratio and colony morphology together possibly with products of accessory cells influence the interpretation of the radiosensitivity parameters.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Survival-
dc.subject.meshCesium Radioisotopes-
dc.subject.meshColony-Stimulating Factors-
dc.subject.meshDose-Response Relationship, Radiation-
dc.subject.meshFemale-
dc.subject.meshGamma Rays-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshRadiation Tolerance-
dc.subject.meshRecombinant Proteins-
dc.titleRadiosensitivity increases with differentiation status of murine hemopoietic progenitor cells selected using enriched marrow subpopulations and recombinant growth factors.en
dc.typeArticleen
dc.contributor.departmentDepartment of Radiobiology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalRadiation Researchen
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