Copper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109819
Title:
Copper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure.
Authors:
Povey, Andrew C; O'Neill, I K
Abstract:
Copper phthalocyanine tetrasulphonic acid (CPTS) functions were introduced into magnetic semi-permeable polyethyleneimine (PEI) microcapsules in order to create a recoverable scavenging system for trapping and biomonitoring, within the gastrointestinal cavity, of mutagens having a planar molecular structure. Stable ionic CPTS and covalent (thionylated CPTS, TCPTS) adducts to the microcapsule PEI were produced and shown to trap benzo[a]pyrene (B[a]P) in vitro in relation to the porphyrin/B[a]P ratio employed. 3-hydroxy B[a]P and B[a]P 3,6-dione from a crude B[a]P metabolite mixture, and a set of planar mutagens from crude opium/morphine pyrolysate mixtures could also be recovered in 7-86% yields after shaking with modified microcapsules followed by methanol/ammonia (50:1) desorption. Tetraols derived from B[a]P 7,8-diol-9,10 epoxide could also be recovered. Modified microcapsules were recovered magnetically from faeces of rats treated with [14C]B[a]P, and 45-51% of trapped radioactivity could be directly desorbed for HPLC assay compared with 30% for unmodified microscapsules. The relative extent of trapping by unmodified or CPTS- or TCPTS-modified microcapsules was different for various substrates, and it appears that the copper phthalocyanine tetrasulphonic acid moiety competes with another unidentified absorption/desorption structure in the microcapsules. These results show that selective and reversible trapping of carcinogens/mutagens having planar molecular structure can be achieved within the gastrointestinal tract.
Affiliation:
Paterson Institute for Cancer Research, Department of Carcinogenesis, Withington, Manchester, UK.
Citation:
Copper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure. 1990, 11 (11):1989-93 Carcinogenesis
Journal:
Carcinogenesis
Issue Date:
Nov-1990
URI:
http://hdl.handle.net/10541/109819
DOI:
10.1093/carcin/11.11.1989
PubMed ID:
2225331
Type:
Article
Language:
en
ISSN:
0143-3334
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorPovey, Andrew Cen
dc.contributor.authorO'Neill, I Ken
dc.date.accessioned2010-08-18T10:07:14Z-
dc.date.available2010-08-18T10:07:14Z-
dc.date.issued1990-11-
dc.identifier.citationCopper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure. 1990, 11 (11):1989-93 Carcinogenesisen
dc.identifier.issn0143-3334-
dc.identifier.pmid2225331-
dc.identifier.doi10.1093/carcin/11.11.1989-
dc.identifier.urihttp://hdl.handle.net/10541/109819-
dc.description.abstractCopper phthalocyanine tetrasulphonic acid (CPTS) functions were introduced into magnetic semi-permeable polyethyleneimine (PEI) microcapsules in order to create a recoverable scavenging system for trapping and biomonitoring, within the gastrointestinal cavity, of mutagens having a planar molecular structure. Stable ionic CPTS and covalent (thionylated CPTS, TCPTS) adducts to the microcapsule PEI were produced and shown to trap benzo[a]pyrene (B[a]P) in vitro in relation to the porphyrin/B[a]P ratio employed. 3-hydroxy B[a]P and B[a]P 3,6-dione from a crude B[a]P metabolite mixture, and a set of planar mutagens from crude opium/morphine pyrolysate mixtures could also be recovered in 7-86% yields after shaking with modified microcapsules followed by methanol/ammonia (50:1) desorption. Tetraols derived from B[a]P 7,8-diol-9,10 epoxide could also be recovered. Modified microcapsules were recovered magnetically from faeces of rats treated with [14C]B[a]P, and 45-51% of trapped radioactivity could be directly desorbed for HPLC assay compared with 30% for unmodified microscapsules. The relative extent of trapping by unmodified or CPTS- or TCPTS-modified microcapsules was different for various substrates, and it appears that the copper phthalocyanine tetrasulphonic acid moiety competes with another unidentified absorption/desorption structure in the microcapsules. These results show that selective and reversible trapping of carcinogens/mutagens having planar molecular structure can be achieved within the gastrointestinal tract.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBenzo(a)pyrene-
dc.subject.meshCapsules-
dc.subject.meshIndoles-
dc.subject.meshMale-
dc.subject.meshMorphine Derivatives-
dc.subject.meshOrganometallic Compounds-
dc.subject.meshPolyethyleneimine-
dc.subject.meshRats-
dc.subject.meshStructure-Activity Relationship-
dc.titleCopper phthalocyanine labelled magnetic microcapsules: preparation, and binding properties in vitro and in vivo for mutagens having planar molecular structure.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Department of Carcinogenesis, Withington, Manchester, UK.en
dc.identifier.journalCarcinogenesisen
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