Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109815
Title:
Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.
Authors:
Rossiter, Belinda J; Muzny, D M; Hampson, Ian N; Caskey, C T; Fox, Margaret
Abstract:
The Chinese hamster hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient cell line TG15 produces apparently normal HPRT mRNA by northern analysis and was therefore presumed to contain a point mutation within the coding region. Sequencing cDNA from the TG15 cell line revealed an A to G transition which results in the substitution of the amino acid glycine for aspartic acid at position 135. TG15 cells revert to wild-type HPRT activity upon exposure to monofunctional alkylating agents. A rapid test to assay the site of the TG15 point mutation has been developed, utilizing the polymerase chain reaction and allele-specific oligonucleotide screening. In all revertants studied, the original point mutation has been corrected to the wild-type sequence. The TG15 point mutation lies within a proposed catalytic domain of the HPRT protein in common with other phosphoribosyltransferases.
Affiliation:
Institute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.
Citation:
Induced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence. 1990, 5 (6):605-8 Mutagenesis
Journal:
Mutagenesis
Issue Date:
Nov-1990
URI:
http://hdl.handle.net/10541/109815
DOI:
10.1093/mutage/5.6.605
PubMed ID:
1702179
Type:
Article
Language:
en
ISSN:
0267-8357
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorRossiter, Belinda Jen
dc.contributor.authorMuzny, D Men
dc.contributor.authorHampson, Ian Nen
dc.contributor.authorCaskey, C Ten
dc.contributor.authorFox, Margareten
dc.date.accessioned2010-08-18T08:33:57Z-
dc.date.available2010-08-18T08:33:57Z-
dc.date.issued1990-11-
dc.identifier.citationInduced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence. 1990, 5 (6):605-8 Mutagenesisen
dc.identifier.issn0267-8357-
dc.identifier.pmid1702179-
dc.identifier.doi10.1093/mutage/5.6.605-
dc.identifier.urihttp://hdl.handle.net/10541/109815-
dc.description.abstractThe Chinese hamster hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient cell line TG15 produces apparently normal HPRT mRNA by northern analysis and was therefore presumed to contain a point mutation within the coding region. Sequencing cDNA from the TG15 cell line revealed an A to G transition which results in the substitution of the amino acid glycine for aspartic acid at position 135. TG15 cells revert to wild-type HPRT activity upon exposure to monofunctional alkylating agents. A rapid test to assay the site of the TG15 point mutation has been developed, utilizing the polymerase chain reaction and allele-specific oligonucleotide screening. In all revertants studied, the original point mutation has been corrected to the wild-type sequence. The TG15 point mutation lies within a proposed catalytic domain of the HPRT protein in common with other phosphoribosyltransferases.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCell Line-
dc.subject.meshCricetinae-
dc.subject.meshCricetulus-
dc.subject.meshDNA-
dc.subject.meshGenes-
dc.subject.meshHypoxanthine Phosphoribosyltransferase-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutation-
dc.subject.meshOligonucleotide Probes-
dc.subject.meshPoly A-
dc.subject.meshRNA-
dc.subject.meshRNA, Messenger-
dc.subject.meshRestriction Mapping-
dc.titleInduced reversion of a spontaneous point mutation within the Chinese hamster HPRT gene to the wild-type sequence.en
dc.typeArticleen
dc.contributor.departmentInstitute for Molecular Genetics, Baylor College of Medicine, Houston, TX 77030.en
dc.identifier.journalMutagenesisen
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