Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109813
Title:
Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.
Authors:
Littler, Edward; Lawrence, G; Liu, Mei-Ying; Barrell, B G; Arrand, John R
Abstract:
DNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.
Affiliation:
Department of Molecular Biology, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.
Citation:
Identification, cloning, and expression of the major capsid protein gene of human herpesvirus 6. 1990, 64 (2):714-22 J. Virol.
Journal:
Journal of Virology
Issue Date:
Feb-1990
URI:
http://hdl.handle.net/10541/109813
PubMed ID:
2153237
Type:
Article
Language:
en
ISSN:
0022-538X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLittler, Edwarden
dc.contributor.authorLawrence, Gen
dc.contributor.authorLiu, Mei-Yingen
dc.contributor.authorBarrell, B Gen
dc.contributor.authorArrand, John Ren
dc.date.accessioned2010-08-18T08:23:55Z-
dc.date.available2010-08-18T08:23:55Z-
dc.date.issued1990-02-
dc.identifier.citationIdentification, cloning, and expression of the major capsid protein gene of human herpesvirus 6. 1990, 64 (2):714-22 J. Virol.en
dc.identifier.issn0022-538X-
dc.identifier.pmid2153237-
dc.identifier.urihttp://hdl.handle.net/10541/109813-
dc.description.abstractDNA sequence analysis of part of the human herpesvirus 6 (HHV-6) genome led to the identification of an open reading frame with amino acid sequence homology to the major capsid proteins (MCP) of other HHVs. DIAGON analysis showed that the closest homology was with human cytomegalovirus. Plasmids were constructed which were shown to express the HHV-6 MCP as either the entire open reading frame or as portions of it, and the recombinant-produced proteins were used to raise antisera. The antisera were shown by immunofluorescence to react with HHV-6-infected lymphoblastoid cells and in Western blots with a 135-kilodalton protein specific to HHV-6-infected cells. The recombinant protein expressed from the entire HHV-6 MCP gene was detected only weakly in Western blot assays with normal HHV-6-positive human sera as a probe.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshBase Sequence-
dc.subject.meshCapsid-
dc.subject.meshCells, Cultured-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA, Viral-
dc.subject.meshFetal Blood-
dc.subject.meshGene Expression-
dc.subject.meshGenes, Viral-
dc.subject.meshHumans-
dc.subject.meshLymphocytes-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPlasmids-
dc.subject.meshRestriction Mapping-
dc.subject.meshSequence Homology, Nucleic Acid-
dc.subject.meshSimplexvirus-
dc.subject.meshViral Structural Proteins-
dc.titleIdentification, cloning, and expression of the major capsid protein gene of human herpesvirus 6.en
dc.typeArticleen
dc.contributor.departmentDepartment of Molecular Biology, Christie Hospital & Holt Radium Institute, Manchester, United Kingdom.en
dc.identifier.journalJournal of Virologyen

Related articles on PubMed

All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.