Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109807
Title:
Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia.
Authors:
Tzavaras, T; Stewart, M; McDougall, A; Fulton, R; Testa, Nydia G; Onions, D E; Neil, J C
Abstract:
The GM1 strain of feline leukaemia virus (FeLV) was isolated from a naturally occurring case of myeloid leukaemia and induces severe haematopoietic abnormalities, including myeloblastic leukaemia, on inoculation into cats. Molecular clones of FeLV-GM1 proviruses were obtained and studied by restriction enzyme mapping, blot hybridization and partial DNA sequence analysis. Two types of clone were isolated; the first was a replication-competent FeLV of subgroup A, resembling other low or minimally pathogenic FeLV-A isolates; the second was replication-defective with extensive deletions and mutations in gag and pol, although it has an intact env gene of subgroup B phenotype. Large segments of the defective proviruses, from the 5' leader sequence upstream of the gag gene to the 5' half of the env gene, show structural hallmarks of endogenous FeLV-related proviruses. Infectious FeLV-GM1 viruses recovered after transfection were tested for their leukaemogenic potential in newborn cats. Early polyclonal myeloproliferative changes were observed in cats inoculated with FeLV-A/GM1 alone, although these were more pronounced in animals receiving the full FeLV-AB/GM1 complex reconstituted by cotransfection of the defective virus FeLV-B with its FeLV-A helper. Analysis of viruses in the bone marrow showed that replication of the subgroup B component is delayed and restricted to a proportion of cats. Most of the infected cats developed persistent abnormalities of haematopoiesis and one progressed to disseminated myeloid leukaemia. The defective recombinant FeLV-B/GM1 appears to play an indirect but important role in myeloid leukaemogenesis.
Affiliation:
Beatson Institute for Cancer Research, Bearsden, Glasgow, U.K.
Citation:
Molecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia. 1990, 71 ( Pt 2):343-54 J. Gen. Virol.
Journal:
Journal of General Virology
Issue Date:
Feb-1990
URI:
http://hdl.handle.net/10541/109807
DOI:
10.1099/0022-1317-71-2-343
PubMed ID:
2155287
Type:
Article
Language:
en
ISSN:
0022-1317
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorTzavaras, Ten
dc.contributor.authorStewart, Men
dc.contributor.authorMcDougall, Aen
dc.contributor.authorFulton, Ren
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorOnions, D Een
dc.contributor.authorNeil, J Cen
dc.date.accessioned2010-08-18T08:57:21Z-
dc.date.available2010-08-18T08:57:21Z-
dc.date.issued1990-02-
dc.identifier.citationMolecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia. 1990, 71 ( Pt 2):343-54 J. Gen. Virol.en
dc.identifier.issn0022-1317-
dc.identifier.pmid2155287-
dc.identifier.doi10.1099/0022-1317-71-2-343-
dc.identifier.urihttp://hdl.handle.net/10541/109807-
dc.description.abstractThe GM1 strain of feline leukaemia virus (FeLV) was isolated from a naturally occurring case of myeloid leukaemia and induces severe haematopoietic abnormalities, including myeloblastic leukaemia, on inoculation into cats. Molecular clones of FeLV-GM1 proviruses were obtained and studied by restriction enzyme mapping, blot hybridization and partial DNA sequence analysis. Two types of clone were isolated; the first was a replication-competent FeLV of subgroup A, resembling other low or minimally pathogenic FeLV-A isolates; the second was replication-defective with extensive deletions and mutations in gag and pol, although it has an intact env gene of subgroup B phenotype. Large segments of the defective proviruses, from the 5' leader sequence upstream of the gag gene to the 5' half of the env gene, show structural hallmarks of endogenous FeLV-related proviruses. Infectious FeLV-GM1 viruses recovered after transfection were tested for their leukaemogenic potential in newborn cats. Early polyclonal myeloproliferative changes were observed in cats inoculated with FeLV-A/GM1 alone, although these were more pronounced in animals receiving the full FeLV-AB/GM1 complex reconstituted by cotransfection of the defective virus FeLV-B with its FeLV-A helper. Analysis of viruses in the bone marrow showed that replication of the subgroup B component is delayed and restricted to a proportion of cats. Most of the infected cats developed persistent abnormalities of haematopoiesis and one progressed to disseminated myeloid leukaemia. The defective recombinant FeLV-B/GM1 appears to play an indirect but important role in myeloid leukaemogenesis.en
dc.language.isoenen
dc.subjectFeline Leukaemia Virusen
dc.subjectMyeloid Leukaemiaen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Southern-
dc.subject.meshCat Diseases-
dc.subject.meshCats-
dc.subject.meshCloning, Molecular-
dc.subject.meshDNA Replication-
dc.subject.meshDNA, Viral-
dc.subject.meshDefective Viruses-
dc.subject.meshGenes, gag-
dc.subject.meshLeukemia Virus, Feline-
dc.subject.meshLeukemia, Myeloid-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshNucleic Acid Hybridization-
dc.subject.meshProviruses-
dc.subject.meshRestriction Mapping-
dc.subject.meshTransfection-
dc.subject.meshVirus Replication-
dc.titleMolecular cloning and characterization of a defective recombinant feline leukaemia virus associated with myeloid leukaemia.en
dc.typeArticleen
dc.contributor.departmentBeatson Institute for Cancer Research, Bearsden, Glasgow, U.K.en
dc.identifier.journalJournal of General Virologyen

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