Biological characterization of recombinant vaccinia viruses in mice infected by the respiratory route.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109806
Title:
Biological characterization of recombinant vaccinia viruses in mice infected by the respiratory route.
Authors:
Williamson, J D; Reith, R W; Jeffrey, L J; Arrand, John R; Mackett, Mike
Abstract:
A murine model based on infection by the respiratory route has been used to study the pathogenesis of recombinant vaccinia viruses. The neurovirulent Western Reserve (WR) strain and the Wyeth smallpox vaccine strain were used as vectors. Recombinant viruses were constructed by insertion of the Epstein-Barr virus membrane glycoprotein 340 gene into the thymidine kinase (TK) gene of each vaccinia virus. Intranasal inoculation of DBA/2 mice with 10(6) pock-forming units (pk.f.u.) of the WR strain was lethal but mice survived similar infection with the WR recombinant virus. Each virus was recovered from lung, blood and brain but, unlike wild-type virus, the recombinant virus was subsequently cleared. No deaths occurred after similar infection with the Wyeth strain or the Wyeth recombinant virus. There was limited growth of the Wyeth strain in the respiratory tract, low levels of virus in the blood and only sporadic recovery in brain extracts. The Wyeth recombinant virus was cleared rapidly with little viraemia or detectable infection of the central nervous system. No phenotypic character determined in vitro could be related consistently to the virulence of wild-type and recombinant viruses. Although the lethal character of the WR strain was affected by its TK+ phenotype, mice survived infection by intranasal inoculation with 10(6) pk.f.u. of WR TK+ recombinant viruses which either expressed the human interleukin 2 gene or had a deficient vaccinia virus growth factor gene.
Affiliation:
Department of Medical Microbiology, St Mary's Hospital Medical School, London, U.K.
Citation:
Biological characterization of recombinant vaccinia viruses in mice infected by the respiratory route. 1990, 71 ( Pt 11):2761-7 J. Gen. Virol.
Journal:
Journal of General Virology
Issue Date:
Nov-1990
URI:
http://hdl.handle.net/10541/109806
DOI:
10.1099/0022-1317-71-11-2761
PubMed ID:
2254756
Type:
Article
Language:
en
ISSN:
0022-1317
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWilliamson, J Den
dc.contributor.authorReith, R Wen
dc.contributor.authorJeffrey, L Jen
dc.contributor.authorArrand, John Ren
dc.contributor.authorMackett, Mikeen
dc.date.accessioned2010-08-18T08:54:12Z-
dc.date.available2010-08-18T08:54:12Z-
dc.date.issued1990-11-
dc.identifier.citationBiological characterization of recombinant vaccinia viruses in mice infected by the respiratory route. 1990, 71 ( Pt 11):2761-7 J. Gen. Virol.en
dc.identifier.issn0022-1317-
dc.identifier.pmid2254756-
dc.identifier.doi10.1099/0022-1317-71-11-2761-
dc.identifier.urihttp://hdl.handle.net/10541/109806-
dc.description.abstractA murine model based on infection by the respiratory route has been used to study the pathogenesis of recombinant vaccinia viruses. The neurovirulent Western Reserve (WR) strain and the Wyeth smallpox vaccine strain were used as vectors. Recombinant viruses were constructed by insertion of the Epstein-Barr virus membrane glycoprotein 340 gene into the thymidine kinase (TK) gene of each vaccinia virus. Intranasal inoculation of DBA/2 mice with 10(6) pock-forming units (pk.f.u.) of the WR strain was lethal but mice survived similar infection with the WR recombinant virus. Each virus was recovered from lung, blood and brain but, unlike wild-type virus, the recombinant virus was subsequently cleared. No deaths occurred after similar infection with the Wyeth strain or the Wyeth recombinant virus. There was limited growth of the Wyeth strain in the respiratory tract, low levels of virus in the blood and only sporadic recovery in brain extracts. The Wyeth recombinant virus was cleared rapidly with little viraemia or detectable infection of the central nervous system. No phenotypic character determined in vitro could be related consistently to the virulence of wild-type and recombinant viruses. Although the lethal character of the WR strain was affected by its TK+ phenotype, mice survived infection by intranasal inoculation with 10(6) pk.f.u. of WR TK+ recombinant viruses which either expressed the human interleukin 2 gene or had a deficient vaccinia virus growth factor gene.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCells, Cultured-
dc.subject.meshCentral Nervous System Diseases-
dc.subject.meshCloning, Molecular-
dc.subject.meshGenes, Viral-
dc.subject.meshGenetic Vectors-
dc.subject.meshHumans-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshRespiratory Tract Infections-
dc.subject.meshSmallpox Vaccine-
dc.subject.meshThymidine Kinase-
dc.subject.meshVaccinia virus-
dc.subject.meshViremia-
dc.titleBiological characterization of recombinant vaccinia viruses in mice infected by the respiratory route.en
dc.typeArticleen
dc.contributor.departmentDepartment of Medical Microbiology, St Mary's Hospital Medical School, London, U.K.en
dc.identifier.journalJournal of General Virologyen
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