Characterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109794
Title:
Characterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment.
Authors:
Mackett, Mike; Conway, Margaret J; Arrand, John R; Haddad, R S; Hutt-Fletcher, L M
Abstract:
Computer-assisted analysis of the Epstein-Barr virus (EBV) open reading frame BILF2 (B95-8 nucleotides 150,525 to 149,782) predicts that it codes for a membrane-bound glycoprotein. [3H]glucosamine labeling of cells infected with vaccinia virus recombinants that expressed the BILF2 open reading frame revealed several diffuse species of glycoproteins of around 80,000 and 55,000 daltons. A monoclonal antibody derived from spleens of mice immunized with EBV immunoprecipitated the EBV-derived protein made by the vaccinia virus recombinants and also precipitated a late envelope glycoprotein with a mobility of 78,000 to 55,000 from EBV-producing cells. N-Glycanase treatment of the immunoprecipitated BILF2 product from EBV-producing cells resulted in a polypeptide of 28 kilodaltons, closely agreeing with the predicted molecular mass for the unmodified BILF2 gene product. Western (immuno-) blots using recombinant infected cells as a source of antigen showed that the majority of EBV-seropositive individuals have a serum antibody response to the BILF2-encoded gp78/55.
Affiliation:
Cancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, United Kingdom.
Citation:
Characterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment. 1990, 64 (6):2545-52 J. Virol.
Journal:
Journal of Virology
Issue Date:
Jun-1990
URI:
http://hdl.handle.net/10541/109794
PubMed ID:
2159529
Type:
Article
Language:
en
ISSN:
0022-538X
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMackett, Mikeen
dc.contributor.authorConway, Margaret Jen
dc.contributor.authorArrand, John Ren
dc.contributor.authorHaddad, R Sen
dc.contributor.authorHutt-Fletcher, L Men
dc.date.accessioned2010-08-18T08:21:30Z-
dc.date.available2010-08-18T08:21:30Z-
dc.date.issued1990-06-
dc.identifier.citationCharacterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment. 1990, 64 (6):2545-52 J. Virol.en
dc.identifier.issn0022-538X-
dc.identifier.pmid2159529-
dc.identifier.urihttp://hdl.handle.net/10541/109794-
dc.description.abstractComputer-assisted analysis of the Epstein-Barr virus (EBV) open reading frame BILF2 (B95-8 nucleotides 150,525 to 149,782) predicts that it codes for a membrane-bound glycoprotein. [3H]glucosamine labeling of cells infected with vaccinia virus recombinants that expressed the BILF2 open reading frame revealed several diffuse species of glycoproteins of around 80,000 and 55,000 daltons. A monoclonal antibody derived from spleens of mice immunized with EBV immunoprecipitated the EBV-derived protein made by the vaccinia virus recombinants and also precipitated a late envelope glycoprotein with a mobility of 78,000 to 55,000 from EBV-producing cells. N-Glycanase treatment of the immunoprecipitated BILF2 product from EBV-producing cells resulted in a polypeptide of 28 kilodaltons, closely agreeing with the predicted molecular mass for the unmodified BILF2 gene product. Western (immuno-) blots using recombinant infected cells as a source of antigen showed that the majority of EBV-seropositive individuals have a serum antibody response to the BILF2-encoded gp78/55.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshBase Sequence-
dc.subject.meshBlotting, Western-
dc.subject.meshCell Line-
dc.subject.meshDeoxyribonuclease BamHI-
dc.subject.meshFluorescent Antibody Technique-
dc.subject.meshGenes, Viral-
dc.subject.meshGlycoproteins-
dc.subject.meshHerpesvirus 4, Human-
dc.subject.meshHumans-
dc.subject.meshImmunoglobulin G-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshPlasmids-
dc.subject.meshRestriction Mapping-
dc.subject.meshVaccinia virus-
dc.subject.meshViral Proteins-
dc.titleCharacterization and expression of a glycoprotein encoded by the Epstein-Barr virus BamHI I fragment.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Laboratories, Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, Manchester, United Kingdom.en
dc.identifier.journalJournal of Virologyen
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