A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109793
Title:
A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver.
Authors:
Lyon, Malcolm; Gallagher, John T
Abstract:
A sensitive method has been developed for the visualization of nonradiolabeled glycosaminoglycan oligosaccharides resolved by polyacrylamide gel electrophoresis using fixation with azure A followed by staining with ammoniacal silver. This method, which can detect as little as 1-2 ng of a single oligosaccharide species, can be used to stain a few micrograms of a complex oligosaccharide mixture. The combination of gradient polyacrylamide gel electrophoresis and sequential azure A/silver staining can be applied to the analysis of all the complex glycosaminoglycans (i.e., heparin, heparan sulfate, chondroitin/dermatan sulfate, keratan sulfate) and hyaluronate, as well as to comparisons of specificities of the glycosaminoglycan-degrading enzymes. This procedure may be particularly valuable in situations where the availability of glycosaminoglycan is very limited and/or where radiolabeling is impractical or undesirable.
Affiliation:
Cancer Research Campaign Department of Medical Oncology, Christie Hospital, Manchester, United Kingdom.
Citation:
A general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver. 1990, 185 (1):63-70 Anal. Biochem.
Journal:
Analytical Biochemistry
Issue Date:
15-Feb-1990
URI:
http://hdl.handle.net/10541/109793
DOI:
10.1016/0003-2697(90)90255-8
PubMed ID:
1693050
Type:
Article
Language:
en
ISSN:
0003-2697
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLyon, Malcolmen
dc.contributor.authorGallagher, John Ten
dc.date.accessioned2010-08-18T08:18:55Z-
dc.date.available2010-08-18T08:18:55Z-
dc.date.issued1990-02-15-
dc.identifier.citationA general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver. 1990, 185 (1):63-70 Anal. Biochem.en
dc.identifier.issn0003-2697-
dc.identifier.pmid1693050-
dc.identifier.doi10.1016/0003-2697(90)90255-8-
dc.identifier.urihttp://hdl.handle.net/10541/109793-
dc.description.abstractA sensitive method has been developed for the visualization of nonradiolabeled glycosaminoglycan oligosaccharides resolved by polyacrylamide gel electrophoresis using fixation with azure A followed by staining with ammoniacal silver. This method, which can detect as little as 1-2 ng of a single oligosaccharide species, can be used to stain a few micrograms of a complex oligosaccharide mixture. The combination of gradient polyacrylamide gel electrophoresis and sequential azure A/silver staining can be applied to the analysis of all the complex glycosaminoglycans (i.e., heparin, heparan sulfate, chondroitin/dermatan sulfate, keratan sulfate) and hyaluronate, as well as to comparisons of specificities of the glycosaminoglycan-degrading enzymes. This procedure may be particularly valuable in situations where the availability of glycosaminoglycan is very limited and/or where radiolabeling is impractical or undesirable.en
dc.language.isoenen
dc.subject.meshAzure Stains-
dc.subject.meshChondroitin Sulfates-
dc.subject.meshDermatan Sulfate-
dc.subject.meshElectrophoresis, Polyacrylamide Gel-
dc.subject.meshGlycosaminoglycans-
dc.subject.meshHeparin-
dc.subject.meshHeparitin Sulfate-
dc.subject.meshKeratan Sulfate-
dc.subject.meshOligosaccharides-
dc.subject.meshParticle Size-
dc.subject.meshPhenothiazines-
dc.subject.meshPolysaccharide-Lyases-
dc.subject.meshSilver-
dc.subject.meshStaining and Labeling-
dc.subject.meshSubstrate Specificity-
dc.titleA general method for the detection and mapping of submicrogram quantities of glycosaminoglycan oligosaccharides on polyacrylamide gels by sequential staining with azure A and ammoniacal silver.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Medical Oncology, Christie Hospital, Manchester, United Kingdom.en
dc.identifier.journalAnalytical Biochemistryen
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