2.50
Hdl Handle:
http://hdl.handle.net/10541/109769
Title:
The synthesis of proteoglycans by human T lymphocytes.
Authors:
Steward, William P; Christmas, Stephen E; Lyon, Malcolm; Gallagher, John T
Abstract:
We have examined the proteoglycans produced by highly-purified cultures of human T-lymphocytes. The proteoglycans were metabolically labelled with [35S]sulphate and analysed in cellular and medium fractions using DEAE-cellulose chromatography, gel filtration and specific enzymatic and chemical degradations. The results showed that the T cells synthesized a relatively homogeneous, proteinase-resistant chondroitin 4-sulphate proteoglycan that accumulated in the culture medium during a 48 h incubation period. The cellular fraction contained a significant amount of free chondroitin sulphate chains that were not secreted into the medium. These polysaccharides were formed by intracellular degradation of proteoglycan in a chloroquine-sensitive process, indicating a requirement for an acidic environment. In contrast to chondroitin sulphate derived from proteoglycan, chondroitin sulphates synthesized on the exogenous primer, beta-D-xyloside, were mainly secreted by the cells. beta-D-Xylosides caused an 8-fold stimulation in the synthesis of chondroitin sulphate, but decreased the synthesis of proteoglycan by about 50%. These proteoglycans contained shorter chondroitin sulphate chains than their normal counterparts. The results indicate that although proteoglycans are mainly secretory components in human T-cell cultures, a specific metabolic step leads to the intracellular accumulation of free glycosaminoglycans. Separate functions are likely to be associated with the intracellular and secretory pools of chondroitin sulphate.
Affiliation:
CRC Dept. Medical Oncology, Christie Hospital, Manchester, U.K.
Citation:
The synthesis of proteoglycans by human T lymphocytes. 1990, 1052 (3):416-25 Biochim. Biophys. Acta
Journal:
Biochimica et Biophysica Acta
Issue Date:
22-May-1990
URI:
http://hdl.handle.net/10541/109769
DOI:
10.1016/0167-4889(90)90151-3
PubMed ID:
2354207
Type:
Article
Language:
en
ISSN:
0006-3002
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSteward, William Pen
dc.contributor.authorChristmas, Stephen Een
dc.contributor.authorLyon, Malcolmen
dc.contributor.authorGallagher, John Ten
dc.date.accessioned2010-08-17T13:39:23Z-
dc.date.available2010-08-17T13:39:23Z-
dc.date.issued1990-05-22-
dc.identifier.citationThe synthesis of proteoglycans by human T lymphocytes. 1990, 1052 (3):416-25 Biochim. Biophys. Actaen
dc.identifier.issn0006-3002-
dc.identifier.pmid2354207-
dc.identifier.doi10.1016/0167-4889(90)90151-3-
dc.identifier.urihttp://hdl.handle.net/10541/109769-
dc.description.abstractWe have examined the proteoglycans produced by highly-purified cultures of human T-lymphocytes. The proteoglycans were metabolically labelled with [35S]sulphate and analysed in cellular and medium fractions using DEAE-cellulose chromatography, gel filtration and specific enzymatic and chemical degradations. The results showed that the T cells synthesized a relatively homogeneous, proteinase-resistant chondroitin 4-sulphate proteoglycan that accumulated in the culture medium during a 48 h incubation period. The cellular fraction contained a significant amount of free chondroitin sulphate chains that were not secreted into the medium. These polysaccharides were formed by intracellular degradation of proteoglycan in a chloroquine-sensitive process, indicating a requirement for an acidic environment. In contrast to chondroitin sulphate derived from proteoglycan, chondroitin sulphates synthesized on the exogenous primer, beta-D-xyloside, were mainly secreted by the cells. beta-D-Xylosides caused an 8-fold stimulation in the synthesis of chondroitin sulphate, but decreased the synthesis of proteoglycan by about 50%. These proteoglycans contained shorter chondroitin sulphate chains than their normal counterparts. The results indicate that although proteoglycans are mainly secretory components in human T-cell cultures, a specific metabolic step leads to the intracellular accumulation of free glycosaminoglycans. Separate functions are likely to be associated with the intracellular and secretory pools of chondroitin sulphate.en
dc.language.isoenen
dc.subject.meshCells, Cultured-
dc.subject.meshChloroquine-
dc.subject.meshGlycosaminoglycans-
dc.subject.meshGlycosides-
dc.subject.meshHumans-
dc.subject.meshProteoglycans-
dc.subject.meshT-Lymphocytes-
dc.titleThe synthesis of proteoglycans by human T lymphocytes.en
dc.typeArticleen
dc.contributor.departmentCRC Dept. Medical Oncology, Christie Hospital, Manchester, U.K.en
dc.identifier.journalBiochimica et Biophysica Actaen

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