Different roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109419
Title:
Different roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients.
Authors:
Spasojević, Ivan; Stević, Zorica; Nikolić-Kokić, Aleksandra; Jones, David R; Blagojević, Dusko; Spasić, Mihajlo B
Abstract:
Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
Affiliation:
Institute for Multidisciplinary Research, University of Belgrade, Kneza Viseslava 1, 11000 Belgrade, Serbia. ivan@cms.bg.ac.rs
Citation:
Different roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. 2010, 15 (2):81-6 Redox Rep
Journal:
Redox Report
Issue Date:
2010
URI:
http://hdl.handle.net/10541/109419
DOI:
10.1179/174329210X12650506623320
PubMed ID:
20500989
Type:
Article
Language:
en
ISSN:
1743-2928
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorSpasojević, Ivanen
dc.contributor.authorStević, Zoricaen
dc.contributor.authorNikolić-Kokić, Aleksandraen
dc.contributor.authorJones, David Ren
dc.contributor.authorBlagojević, Duskoen
dc.contributor.authorSpasić, Mihajlo Ben
dc.date.accessioned2010-08-10T16:01:18Z-
dc.date.available2010-08-10T16:01:18Z-
dc.date.issued2010-
dc.identifier.citationDifferent roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. 2010, 15 (2):81-6 Redox Repen
dc.identifier.issn1743-2928-
dc.identifier.pmid20500989-
dc.identifier.doi10.1179/174329210X12650506623320-
dc.identifier.urihttp://hdl.handle.net/10541/109419-
dc.description.abstractFerrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H(2)O(2), which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H(2)O(2) to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.en
dc.language.isoenen
dc.subjectAmyotrophic Lateral Sclerosisen
dc.subjectIronen
dc.subjectAscorbateen
dc.subjectUrateen
dc.subject.meshAmyotrophic Lateral Sclerosis-
dc.subject.meshAscorbic Acid-
dc.subject.meshCerebrospinal Fluid-
dc.subject.meshFree Radical Scavengers-
dc.subject.meshUric Acid-
dc.titleDifferent roles of radical scavengers--ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients.en
dc.typeArticleen
dc.contributor.departmentInstitute for Multidisciplinary Research, University of Belgrade, Kneza Viseslava 1, 11000 Belgrade, Serbia. ivan@cms.bg.ac.rsen
dc.identifier.journalRedox Reporten

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