Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109311
Title:
Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples.
Authors:
Bassan, Paul; Kohler, Achim; Martens, Harald; Lee, Joe; Byrne, Hugh J; Dumas, Paul; Gazi, Ehsan; Brown, Michael D; Clarke, Noel W ( 0000-0001-7776-8059 ) ; Gardner, Peter
Abstract:
Infrared spectra of single biological cells often exhibit the 'dispersion artefact' observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at approximately 1655 cm(-1), causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the 'dispersion artefact' which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the 'dispersion artefact' appears to be removed; however, the correction is not perfect. An iterative approach was subsequently implemented whereby the reference spectrum is improved after each iteration, resulting in a more accurate correction. Consequently the corrected spectra become increasingly more representative of the pure absorbance spectra. Using this correction method reliable peak positions can be obtained.
Affiliation:
School of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.
Citation:
Resonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples. 2010, 135 (2):268-77 Analyst
Journal:
The Analyst
Issue Date:
Feb-2010
URI:
http://hdl.handle.net/10541/109311
DOI:
10.1039/b921056c
PubMed ID:
20098758
Type:
Article
Language:
en
ISSN:
1364-5528
Appears in Collections:
All Christie Publications ; All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBassan, Paulen
dc.contributor.authorKohler, Achimen
dc.contributor.authorMartens, Haralden
dc.contributor.authorLee, Joeen
dc.contributor.authorByrne, Hugh Jen
dc.contributor.authorDumas, Paulen
dc.contributor.authorGazi, Ehsanen
dc.contributor.authorBrown, Michael Den
dc.contributor.authorClarke, Noel Wen
dc.contributor.authorGardner, Peteren
dc.date.accessioned2010-08-09T16:11:26Z-
dc.date.available2010-08-09T16:11:26Z-
dc.date.issued2010-02-
dc.identifier.citationResonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples. 2010, 135 (2):268-77 Analysten
dc.identifier.issn1364-5528-
dc.identifier.pmid20098758-
dc.identifier.doi10.1039/b921056c-
dc.identifier.urihttp://hdl.handle.net/10541/109311-
dc.description.abstractInfrared spectra of single biological cells often exhibit the 'dispersion artefact' observed as a sharp decrease in intensity on the high wavenumber side of absorption bands, in particular the Amide I band at approximately 1655 cm(-1), causing a downward shift of the true peak position. The presence of this effect makes any biochemical interpretation of the spectra unreliable. Recent theory has shed light on the origins of the 'dispersion artefact' which has been attributed to resonant Mie scattering (RMieS). In this paper a preliminary algorithm for correcting RMieS is presented and evaluated using simulated data. Results show that the 'dispersion artefact' appears to be removed; however, the correction is not perfect. An iterative approach was subsequently implemented whereby the reference spectrum is improved after each iteration, resulting in a more accurate correction. Consequently the corrected spectra become increasingly more representative of the pure absorbance spectra. Using this correction method reliable peak positions can be obtained.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subjectProstatic Canceren
dc.subject.meshAlgorithms-
dc.subject.meshComputer Simulation-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshProstatic Neoplasms-
dc.subject.meshReference Standards-
dc.subject.meshSpectroscopy, Fourier Transform Infrared-
dc.subject.meshTumor Cells, Cultured-
dc.titleResonant Mie scattering (RMieS) correction of infrared spectra from highly scattering biological samples.en
dc.typeArticleen
dc.contributor.departmentSchool of Chemical Engineering and Analytical Science, Manchester Interdisciplinary Biocentre, University of Manchester, 131 Princess Street, Manchester, UK M1 7DN.en
dc.identifier.journalThe Analysten

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