Cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109030
Title:
Cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line.
Authors:
Harmon, B V; Takano, Y S; Winterford, C M; Potten, Christopher S
Abstract:
Although vincristine is widely used clinically in the treatment of some human cancers, its mechanism of action has not been clearly established. In this study, the patterns of cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line were investigated by light and electron microscopy. Vincristine was found to enhance apoptosis of interphase cells in both systems and also to cause the arrest of cells in mitosis, the latter effect being more pronounced in the intestinal crypts. Arrested mitotic cells went on to die by a process that had a number of features in common with apoptosis. These include compaction of chromatin (following coalescence of chromosomes), condensation of the cytoplasm, initial preservation of organelle integrity, and eventually the fragmentation of the cell into a number of membrane-enclosed bodies which are morphologically similar to conventional apoptotic bodies. The results suggest that the cytocidal effect of vincristine is not solely dependent on metaphase arrest but is a cumulative one, resulting both from apoptosis of interphase cells and the 'apoptotic-like' death of cells arrested in metaphase.
Affiliation:
Department of Pathology, University of Queensland Medical School, Herston, Brisbane, Australia.
Citation:
Cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line. 1992, 25 (6):523-36 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
Nov-1992
URI:
http://hdl.handle.net/10541/109030
DOI:
10.1111/j.1365-2184.1992.tb01457.x
PubMed ID:
1457603
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHarmon, B Ven
dc.contributor.authorTakano, Y Sen
dc.contributor.authorWinterford, C Men
dc.contributor.authorPotten, Christopher Sen
dc.date.accessioned2010-08-04T10:01:16Z-
dc.date.available2010-08-04T10:01:16Z-
dc.date.issued1992-11-
dc.identifier.citationCell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line. 1992, 25 (6):523-36 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid1457603-
dc.identifier.doi10.1111/j.1365-2184.1992.tb01457.x-
dc.identifier.urihttp://hdl.handle.net/10541/109030-
dc.description.abstractAlthough vincristine is widely used clinically in the treatment of some human cancers, its mechanism of action has not been clearly established. In this study, the patterns of cell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line were investigated by light and electron microscopy. Vincristine was found to enhance apoptosis of interphase cells in both systems and also to cause the arrest of cells in mitosis, the latter effect being more pronounced in the intestinal crypts. Arrested mitotic cells went on to die by a process that had a number of features in common with apoptosis. These include compaction of chromatin (following coalescence of chromosomes), condensation of the cytoplasm, initial preservation of organelle integrity, and eventually the fragmentation of the cell into a number of membrane-enclosed bodies which are morphologically similar to conventional apoptotic bodies. The results suggest that the cytocidal effect of vincristine is not solely dependent on metaphase arrest but is a cumulative one, resulting both from apoptosis of interphase cells and the 'apoptotic-like' death of cells arrested in metaphase.en
dc.language.isoenen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAnimals-
dc.subject.meshApoptosis-
dc.subject.meshCell Count-
dc.subject.meshDose-Response Relationship, Drug-
dc.subject.meshHumans-
dc.subject.meshIntestinal Mucosa-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshMitosis-
dc.subject.meshNecrosis-
dc.subject.meshTime Factors-
dc.subject.meshTumor Cells, Cultured-
dc.subject.meshVincristine-
dc.titleCell death induced by vincristine in the intestinal crypts of mice and in a human Burkitt's lymphoma cell line.en
dc.typeArticleen
dc.contributor.departmentDepartment of Pathology, University of Queensland Medical School, Herston, Brisbane, Australia.en
dc.identifier.journalCell Proliferationen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.