Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109029
Title:
Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat.
Authors:
Allan, D J; Harmon, B V; Roberts, Stephen A
Abstract:
In this study we examined the possibility that regular or circadian fluctuations occur in the frequency of spontaneous spermatogonial apoptosis. Apoptosis of A2, A3 and A4 type spermatogonia occurring spontaneously in the normal rat testis was studied by light and electron microscopy. Normal and apoptotic A3 spermatogonia were quantified in 36 animals killed at two-hourly intervals over a 24 h period. Three sequential phases of spermatogonial apoptosis were defined and quantified separately: (i) an early phase in which cells showed margination of nuclear chromatin, (ii) an intermediate phase in which phagocytosed apoptotic bodies were partly degraded and (iii) a late phase in which only debris of degraded apoptotic bodies was evident. Groups of spermatogonia linked by intercellular bridges underwent apoptosis synchronously. Normal and apoptotic A3 spermatogonia occurred at a mean frequency of 33.4 and 9.6 per 10 seminiferous tubule profiles respectively; there was a large variation in these frequencies between animals, but no peaks or circadian periodicity were detected. Progressive degradation of apoptotic bodies was evident, the average ratios of intermediate and late bodies to early bodies being 1.5 and 3.5, respectively. Absence of a circadian rhythm in these data does not exclude the possibility that initiation of apoptosis in susceptible spermatogonial clones is synchronous, and that affected clones undergo lag periods of differing duration before expressing morphological apoptosis.
Affiliation:
Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.
Citation:
Spermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat. 1992, 25 (3):241-50 Cell Prolif.
Journal:
Cell Proliferation
Issue Date:
May-1992
URI:
http://hdl.handle.net/10541/109029
DOI:
10.1111/j.1365-2184.1992.tb01399.x
PubMed ID:
1596537
Type:
Article
Language:
en
ISSN:
0960-7722
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorAllan, D Jen
dc.contributor.authorHarmon, B Ven
dc.contributor.authorRoberts, Stephen Aen
dc.date.accessioned2010-08-04T09:59:36Z-
dc.date.available2010-08-04T09:59:36Z-
dc.date.issued1992-05-
dc.identifier.citationSpermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat. 1992, 25 (3):241-50 Cell Prolif.en
dc.identifier.issn0960-7722-
dc.identifier.pmid1596537-
dc.identifier.doi10.1111/j.1365-2184.1992.tb01399.x-
dc.identifier.urihttp://hdl.handle.net/10541/109029-
dc.description.abstractIn this study we examined the possibility that regular or circadian fluctuations occur in the frequency of spontaneous spermatogonial apoptosis. Apoptosis of A2, A3 and A4 type spermatogonia occurring spontaneously in the normal rat testis was studied by light and electron microscopy. Normal and apoptotic A3 spermatogonia were quantified in 36 animals killed at two-hourly intervals over a 24 h period. Three sequential phases of spermatogonial apoptosis were defined and quantified separately: (i) an early phase in which cells showed margination of nuclear chromatin, (ii) an intermediate phase in which phagocytosed apoptotic bodies were partly degraded and (iii) a late phase in which only debris of degraded apoptotic bodies was evident. Groups of spermatogonia linked by intercellular bridges underwent apoptosis synchronously. Normal and apoptotic A3 spermatogonia occurred at a mean frequency of 33.4 and 9.6 per 10 seminiferous tubule profiles respectively; there was a large variation in these frequencies between animals, but no peaks or circadian periodicity were detected. Progressive degradation of apoptotic bodies was evident, the average ratios of intermediate and late bodies to early bodies being 1.5 and 3.5, respectively. Absence of a circadian rhythm in these data does not exclude the possibility that initiation of apoptosis in susceptible spermatogonial clones is synchronous, and that affected clones undergo lag periods of differing duration before expressing morphological apoptosis.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshCell Death-
dc.subject.meshCircadian Rhythm-
dc.subject.meshMale-
dc.subject.meshRats-
dc.subject.meshRats, Inbred Strains-
dc.subject.meshSpermatogenesis-
dc.subject.meshSpermatogonia-
dc.titleSpermatogonial apoptosis has three morphologically recognizable phases and shows no circadian rhythm during normal spermatogenesis in the rat.en
dc.typeArticleen
dc.contributor.departmentCentre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Australia.en
dc.identifier.journalCell Proliferationen
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