Promutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109026
Title:
Promutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals.
Authors:
Badawi, Alaa F; Mostafa, M H; Aboul-Azm, T; Haboubi, N Y; O'Connor, Peter J; Cooper, Donald P
Abstract:
Radioimmunoassays (RIAs) have been used to detect the promutagenic lesion O6-methyldeoxyguanosine (O6-MedG) in DNA isolated from the bladder tissues of Egyptian patients presenting with bladder carcinoma and concomitant schistosomiasis (bilharziasis), a parasitic disease known to be associated with the presence of N-nitrosamines in the urine. Alkylation damage was present in the DNA of the majority of the samples (44/46, 96%); 38 samples were of tumour tissue and 8 from uninvolved bladder mucosa. Levels of O6-MedG ranged from undetectable (ND; i.e. less than 0.01 mumol O6-MedG/mol dG) to 0.485 mumol/mol dG with an overall mean of 0.134 +/- 0.10 mumol/mol dG, including the two samples that were below the limit of detection. In contrast, methylation damage was detected in only 4/12 (33%) of the DNA samples from normal bladder tissue of European origin. In these samples levels of O6-MedG ranged from ND to 0.225 mumol/mol dG with an overall mean of 0.046 +/- 0.082 mumol O6-MedG/mol dG. These results confirm that alkylation events can be detected in the DNA of schistosome-infected human bladder tissue. The methylation of uninvolved and tumour tissue DNA to similar extents suggests that the alkylating intermediate may have been present in the urine. These results indicate the need for further investigation to determine whether relationships exist between levels of DNA damage and the prevalence of schistosome infection and/or the extent and type of bacterial infection that frequently accompanies this disease.
Affiliation:
Institute for Graduate Studies and Research, University of Alexandria, Egypt.
Citation:
Promutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals. 1992, 13 (5):877-81 Carcinogenesis
Journal:
Carcinogenesis
Issue Date:
May-1992
URI:
http://hdl.handle.net/10541/109026
DOI:
10.1093/carcin/13.5.877
PubMed ID:
1587002
Type:
Article
Language:
en
ISSN:
0143-3334
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBadawi, Alaa Fen
dc.contributor.authorMostafa, M Hen
dc.contributor.authorAboul-Azm, Ten
dc.contributor.authorHaboubi, N Yen
dc.contributor.authorO'Connor, Peter Jen
dc.contributor.authorCooper, Donald Pen
dc.date.accessioned2010-08-04T09:52:11Z-
dc.date.available2010-08-04T09:52:11Z-
dc.date.issued1992-05-
dc.identifier.citationPromutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals. 1992, 13 (5):877-81 Carcinogenesisen
dc.identifier.issn0143-3334-
dc.identifier.pmid1587002-
dc.identifier.doi10.1093/carcin/13.5.877-
dc.identifier.urihttp://hdl.handle.net/10541/109026-
dc.description.abstractRadioimmunoassays (RIAs) have been used to detect the promutagenic lesion O6-methyldeoxyguanosine (O6-MedG) in DNA isolated from the bladder tissues of Egyptian patients presenting with bladder carcinoma and concomitant schistosomiasis (bilharziasis), a parasitic disease known to be associated with the presence of N-nitrosamines in the urine. Alkylation damage was present in the DNA of the majority of the samples (44/46, 96%); 38 samples were of tumour tissue and 8 from uninvolved bladder mucosa. Levels of O6-MedG ranged from undetectable (ND; i.e. less than 0.01 mumol O6-MedG/mol dG) to 0.485 mumol/mol dG with an overall mean of 0.134 +/- 0.10 mumol/mol dG, including the two samples that were below the limit of detection. In contrast, methylation damage was detected in only 4/12 (33%) of the DNA samples from normal bladder tissue of European origin. In these samples levels of O6-MedG ranged from ND to 0.225 mumol/mol dG with an overall mean of 0.046 +/- 0.082 mumol O6-MedG/mol dG. These results confirm that alkylation events can be detected in the DNA of schistosome-infected human bladder tissue. The methylation of uninvolved and tumour tissue DNA to similar extents suggests that the alkylating intermediate may have been present in the urine. These results indicate the need for further investigation to determine whether relationships exist between levels of DNA damage and the prevalence of schistosome infection and/or the extent and type of bacterial infection that frequently accompanies this disease.en
dc.language.isoenen
dc.subjectCancer DNAen
dc.subjectUrinary Bladder Canceren
dc.subject.meshAge Factors-
dc.subject.meshDNA Damage-
dc.subject.meshDNA, Neoplasm-
dc.subject.meshDeoxyguanosine-
dc.subject.meshEgypt-
dc.subject.meshFemale-
dc.subject.meshHumans-
dc.subject.meshMale-
dc.subject.meshMethylation-
dc.subject.meshSchistosomiasis haematobia-
dc.subject.meshSex Factors-
dc.subject.meshUrinary Bladder Neoplasms-
dc.titlePromutagenic methylation damage in bladder DNA from patients with bladder cancer associated with schistosomiasis and from normal individuals.en
dc.typeArticleen
dc.contributor.departmentInstitute for Graduate Studies and Research, University of Alexandria, Egypt.en
dc.identifier.journalCarcinogenesisen

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