Vascular function and tissue injury in murine skin following hyperthermia and photodynamic therapy, alone and in combination.

2.50
Hdl Handle:
http://hdl.handle.net/10541/109015
Title:
Vascular function and tissue injury in murine skin following hyperthermia and photodynamic therapy, alone and in combination.
Authors:
Moore, James V; West, Catharine M L; Haylett, Ann K
Abstract:
The murine tail has been used as a model for injury to skin when hyperthermia (HT) and photodynamic therapy (PDT) using haematoporphyrin derivative, are used in combination. Skin injury by either agent alone was quantitated by the probability of tail necrosis as a function of dose of agent. 'Tolerance' doses of each modality were given and changes in skin vascular function were measured by the rate of clearance of 133Xenon. This was promptly inhibited but restored to normal by 7 days. The absolute numbers of hypodermal vessels of different sizes were measured in tail cross-sections and capillary numbers were found to be greatly reduced between 1 and 7 days, and restored to normal by 21-28 days. When a tolerance dose of PDT was followed at 1, 7, 21 and 28 days by test doses of HT, or vice versa, marked enhancements in probability of necrosis were observed when the interval was 1 or 7 days (Enhancement ratio (ER)PDT-HT = 1.5 and ERHT-PDT = 1.8). Prolonging the interval between modalities to 21-28 days spared the tissue (ERHT-PDT/21 DAYS = 1.1; ERPDT-HT/28 DAYS = 1.0). Close temporal apposition of PDT and HT, such as has been advocated to improve tumour control, may also increase injury to normal tissue through vascular effects common to both.
Affiliation:
Paterson Institute for Cancer Research (Cancer Research Campaign), Christie Hospital (NHS) Trust, Manchester, UK.
Citation:
Vascular function and tissue injury in murine skin following hyperthermia and photodynamic therapy, alone and in combination. 1992, 66 (6):1037-43 Br. J. Cancer
Journal:
British Journal of Cancer
Issue Date:
Dec-1992
URI:
http://hdl.handle.net/10541/109015
PubMed ID:
1457342
Type:
Article
Language:
en
ISSN:
0007-0920
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorMoore, James Ven
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorHaylett, Ann Ken
dc.date.accessioned2010-08-04T09:04:28Z-
dc.date.available2010-08-04T09:04:28Z-
dc.date.issued1992-12-
dc.identifier.citationVascular function and tissue injury in murine skin following hyperthermia and photodynamic therapy, alone and in combination. 1992, 66 (6):1037-43 Br. J. Canceren
dc.identifier.issn0007-0920-
dc.identifier.pmid1457342-
dc.identifier.urihttp://hdl.handle.net/10541/109015-
dc.description.abstractThe murine tail has been used as a model for injury to skin when hyperthermia (HT) and photodynamic therapy (PDT) using haematoporphyrin derivative, are used in combination. Skin injury by either agent alone was quantitated by the probability of tail necrosis as a function of dose of agent. 'Tolerance' doses of each modality were given and changes in skin vascular function were measured by the rate of clearance of 133Xenon. This was promptly inhibited but restored to normal by 7 days. The absolute numbers of hypodermal vessels of different sizes were measured in tail cross-sections and capillary numbers were found to be greatly reduced between 1 and 7 days, and restored to normal by 21-28 days. When a tolerance dose of PDT was followed at 1, 7, 21 and 28 days by test doses of HT, or vice versa, marked enhancements in probability of necrosis were observed when the interval was 1 or 7 days (Enhancement ratio (ER)PDT-HT = 1.5 and ERHT-PDT = 1.8). Prolonging the interval between modalities to 21-28 days spared the tissue (ERHT-PDT/21 DAYS = 1.1; ERPDT-HT/28 DAYS = 1.0). Close temporal apposition of PDT and HT, such as has been advocated to improve tumour control, may also increase injury to normal tissue through vascular effects common to both.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshHyperthermia, Induced-
dc.subject.meshMale-
dc.subject.meshMice-
dc.subject.meshNecrosis-
dc.subject.meshPhotochemotherapy-
dc.subject.meshSkin-
dc.subject.meshTail-
dc.titleVascular function and tissue injury in murine skin following hyperthermia and photodynamic therapy, alone and in combination.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research (Cancer Research Campaign), Christie Hospital (NHS) Trust, Manchester, UK.en
dc.identifier.journalBritish Journal of Canceren
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