Immunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108945
Title:
Immunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.
Authors:
Ford, A M; Bennett, C A; Healy, L E; Navarro, E; Spooncer, Elaine; Greaves, M F
Abstract:
Multipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.
Affiliation:
Leukaemia Research Fund Centre, Chester Beatty Laboratories, London, United Kingdom.
Citation:
Immunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells. 1992, 89 (8):3424-8 Proc. Natl. Acad. Sci. U.S.A.
Journal:
Proceedings of the National Academy of Sciences of the United States of America
Issue Date:
15-Apr-1992
URI:
http://hdl.handle.net/10541/108945
PubMed ID:
1533043
Type:
Article
Language:
en
ISSN:
0027-8424
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorFord, A Men
dc.contributor.authorBennett, C Aen
dc.contributor.authorHealy, L Een
dc.contributor.authorNavarro, Een
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorGreaves, M Fen
dc.date.accessioned2010-08-03T11:53:57Z-
dc.date.available2010-08-03T11:53:57Z-
dc.date.issued1992-04-15-
dc.identifier.citationImmunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells. 1992, 89 (8):3424-8 Proc. Natl. Acad. Sci. U.S.A.en
dc.identifier.issn0027-8424-
dc.identifier.pmid1533043-
dc.identifier.urihttp://hdl.handle.net/10541/108945-
dc.description.abstractMultipotential interleukin 3-dependent non-immortalized murine hemopoietic progenitor cells have DNase I-hypersensitive sites in the immunoglobulin heavy-chain and CD3 delta enhancers and transcribe germ-line T-cell antigen receptor gamma-chain (TCR gamma), but not IgM or TCR beta, genes. Induction of myeloid differentiation in these cells clones down expression and/or transcriptional accessibility of the immunoglobulin heavy-chain and TCR gamma genes. The CD3 delta enhancer region remains DNase I-hypersensitive but closes down in B cells. In embryonic stem cells and pan-mesodermal cells, these genes or enhancer regions are neither expressed nor DNase I-hypersensitive. These data suggest that lineage potential may be programmed, at least in part, by alterations in the accessibility or conformation of regulatory regions of genes and that some promiscuity of gene expression and/or accessibility can precede lineage commitment and maturation in progenitor cells induced to self-renew by interleukin 3.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshAnimals-
dc.subject.meshAntigens, CD3-
dc.subject.meshAntigens, Differentiation, T-Lymphocyte-
dc.subject.meshBone Marrow-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line, Transformed-
dc.subject.meshCell Nucleus-
dc.subject.meshCells, Cultured-
dc.subject.meshDeoxyribonuclease I-
dc.subject.meshEnhancer Elements, Genetic-
dc.subject.meshGene Expression-
dc.subject.meshGenes, Immunoglobulin-
dc.subject.meshGranulocytes-
dc.subject.meshGrowth Substances-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshImmunoglobulin Heavy Chains-
dc.subject.meshInterleukin-3-
dc.subject.meshMethylation-
dc.subject.meshMice-
dc.subject.meshReceptors, Antigen, T-Cell-
dc.subject.meshRestriction Mapping-
dc.subject.meshT-Lymphocytes-
dc.subject.meshTranscription, Genetic-
dc.titleImmunoglobulin heavy-chain and CD3 delta-chain gene enhancers are DNase I-hypersensitive in hemopoietic progenitor cells.en
dc.typeArticleen
dc.contributor.departmentLeukaemia Research Fund Centre, Chester Beatty Laboratories, London, United Kingdom.en
dc.identifier.journalProceedings of the National Academy of Sciences of the United States of Americaen

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