High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108942
Title:
High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.
Authors:
Goldberg, Martin W; Allen, Terence D
Abstract:
The nuclear envelope (NE) of amphibian oocytes can be readily isolated in relatively structurally intact and pure form and has been used extensively for structural studies. Using high resolution scanning electron microscopy (HRSEM), both surfaces of the NE can be visualized in detail. Here, we demonstrate the use of HRSEM to obtain high resolution information of NE structure, confirming previous data and providing some new information. NEs, manually isolated from Triturus cristatus oocytes, have been mounted on conductive silicon chips, fixed, critical point dried and coated with a thin, continuous film of chromium or tantalum and viewed at relatively high accelerating voltage in a field emission scanning electron microscope with the sample within the objective lens. Both nucleoplasmic and cytoplasmic surfaces of the nuclear pore complexes (NPC) have been visualized, revealing the cytoplasmic coaxial ring, associated particles, central plug/transporter and spokes. The nucleoplasmic face is dominated by the previously described basketlike structure attached to the nucleoplasmic coaxial ring. In Triturus, a novel, highly regular flat sheet of fibers, termed the NE lattice (NEL) has been observed attached to the distal ring of the NPC basket. The NEL appears to be distinct from the nuclear lamina. Evidence for the NEL is also presented in thin TEM sections from Triturus oocytes and GVs and in spread NEs from Xenopus. A model is presented for NEL structure and its interaction with the NPCs is discussed.
Affiliation:
Cancer Research Campaign (CRC) Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital National Health Service (NHS) Trust, Manchester, United Kingdom.
Citation:
High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores. 1992, 119 (6):1429-40 J. Cell Biol.
Journal:
Journal of Cell Biology
Issue Date:
Dec-1992
URI:
http://hdl.handle.net/10541/108942
PubMed ID:
1469043
Type:
Article
Language:
en
ISSN:
0021-9525
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorGoldberg, Martin Wen
dc.contributor.authorAllen, Terence Den
dc.date.accessioned2010-08-03T11:32:03Z-
dc.date.available2010-08-03T11:32:03Z-
dc.date.issued1992-12-
dc.identifier.citationHigh resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores. 1992, 119 (6):1429-40 J. Cell Biol.en
dc.identifier.issn0021-9525-
dc.identifier.pmid1469043-
dc.identifier.urihttp://hdl.handle.net/10541/108942-
dc.description.abstractThe nuclear envelope (NE) of amphibian oocytes can be readily isolated in relatively structurally intact and pure form and has been used extensively for structural studies. Using high resolution scanning electron microscopy (HRSEM), both surfaces of the NE can be visualized in detail. Here, we demonstrate the use of HRSEM to obtain high resolution information of NE structure, confirming previous data and providing some new information. NEs, manually isolated from Triturus cristatus oocytes, have been mounted on conductive silicon chips, fixed, critical point dried and coated with a thin, continuous film of chromium or tantalum and viewed at relatively high accelerating voltage in a field emission scanning electron microscope with the sample within the objective lens. Both nucleoplasmic and cytoplasmic surfaces of the nuclear pore complexes (NPC) have been visualized, revealing the cytoplasmic coaxial ring, associated particles, central plug/transporter and spokes. The nucleoplasmic face is dominated by the previously described basketlike structure attached to the nucleoplasmic coaxial ring. In Triturus, a novel, highly regular flat sheet of fibers, termed the NE lattice (NEL) has been observed attached to the distal ring of the NPC basket. The NEL appears to be distinct from the nuclear lamina. Evidence for the NEL is also presented in thin TEM sections from Triturus oocytes and GVs and in spread NEs from Xenopus. A model is presented for NEL structure and its interaction with the NPCs is discussed.en
dc.language.isoenen
dc.subject.meshAmphibians-
dc.subject.meshAnimals-
dc.subject.meshMicroscopy, Electron, Scanning-
dc.subject.meshNuclear Envelope-
dc.subject.meshOocytes-
dc.subject.meshOvum-
dc.subject.meshSubcellular Fractions-
dc.subject.meshTriturus-
dc.subject.meshXenopus laevis-
dc.titleHigh resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign (CRC) Department of Structural Cell Biology, Paterson Institute for Cancer Research, Christie Hospital National Health Service (NHS) Trust, Manchester, United Kingdom.en
dc.identifier.journalJournal of Cell Biologyen
All Items in Christie are protected by copyright, with all rights reserved, unless otherwise indicated.