Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108933
Title:
Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines.
Authors:
Hampson, Ian N; Cross, Michael A; Heyworth, Clare M; Fairbairn, Leslie J; Spooncer, Elaine; Cowling, Graham J; Dexter, T Michael
Abstract:
Using the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3.
Affiliation:
Cancer Research Campaign Department of Experimental Hematology, Paterson Institute for Cancer Research, Withington, Manchester, UK.
Citation:
Expression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines. 1992, 80 (12):3097-105 Blood
Journal:
Blood
Issue Date:
15-Dec-1992
URI:
http://hdl.handle.net/10541/108933
PubMed ID:
1281690
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHampson, Ian Nen
dc.contributor.authorCross, Michael Aen
dc.contributor.authorHeyworth, Clare Men
dc.contributor.authorFairbairn, Leslie Jen
dc.contributor.authorSpooncer, Elaineen
dc.contributor.authorCowling, Graham Jen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-08-03T11:05:03Z-
dc.date.available2010-08-03T11:05:03Z-
dc.date.issued1992-12-15-
dc.identifier.citationExpression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines. 1992, 80 (12):3097-105 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid1281690-
dc.identifier.urihttp://hdl.handle.net/10541/108933-
dc.description.abstractUsing the technique of differential cDNA library screening, we have molecularly cloned a gene that is highly expressed in an undifferentiated myeloid multipotent and growth factor-dependent stem cell line (FDCP-Mix) and that downregulates as these cells are induced to differentiate along monocytic, granulocytic, and erythroid cell lineages. Sequence analysis of this gene has shown homology with a previously cloned gene, cytotoxic cell protease 1 (CCP1 or Granzyme 'B'), that has been shown to be expressed only in thymocytes, activated T cells, a mast cell line, and peritoneal exudate leukocytes. In situ hybridization, Northern blot analysis, and nuclear run-off assay has confirmed that expression of CCP1 is restricted to the phenotypically primitive multipotent undifferentiated. FDCP-Mix cells that are undergoing self-renewal in the presence of growth factors such as interleukin-3.en
dc.language.isoenen
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshBase Sequence-
dc.subject.meshCell Differentiation-
dc.subject.meshCell Line-
dc.subject.meshCloning, Molecular-
dc.subject.meshGene Expression Regulation, Enzymologic-
dc.subject.meshGene Library-
dc.subject.meshGranulocyte Colony-Stimulating Factor-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshGranzymes-
dc.subject.meshGrowth Substances-
dc.subject.meshInterleukin-3-
dc.subject.meshInterleukin-6-
dc.subject.meshMacrophage Colony-Stimulating Factor-
dc.subject.meshMice-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshOpen Reading Frames-
dc.subject.meshRNA-
dc.subject.meshRecombinant Proteins-
dc.subject.meshSerine Endopeptidases-
dc.subject.meshStem Cells-
dc.subject.meshTranscription, Genetic-
dc.subject.meshTransforming Growth Factor beta-
dc.titleExpression and downregulation of cytotoxic cell protease 1 or Granzyme 'B' transcripts during myeloid differentiation of interleukin-3-dependent murine stem cell lines.en
dc.typeArticleen
dc.contributor.departmentCancer Research Campaign Department of Experimental Hematology, Paterson Institute for Cancer Research, Withington, Manchester, UK.en
dc.identifier.journalBlooden
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