Macrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108905
Title:
Macrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo.
Authors:
Lord, Brian I; Dexter, T Michael; Clements, J M; Hunter, M A; Gearing, A J
Abstract:
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) has been assessed for its potential in vivo to protect hematopoietic progenitor cells from the cytotoxic effects of a cycle-specific drug--in this case hydroxyurea (HU). Two doses of HU, 7 hours apart, were administered to mice to induce spleen colony-forming unit (CFU-S) cycling and then to kill them during DNA-synthesis. MIP-1 alpha, in a variety of dose and time combinations, was injected before the second dose of HU in an attempt to prevent recruitment or maintain CFU-S quiescence, and thus protect them from the second dose of HU. Without MIP-1 alpha, recovery of the CFU-S population was complete in 7 days. In a dose-dependent manner, MIP-1 alpha either reduced the initial kill and accelerated recovery, or completely protected the CFU-S population. We conclude that MIP-1 alpha does protect multipotent progenitor cells in vivo and that these observations provide a base from which to build practical clinical applications.
Affiliation:
CRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.
Citation:
Macrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo. 1992, 79 (10):2605-9 Blood
Journal:
Blood
Issue Date:
15-May-1992
URI:
http://hdl.handle.net/10541/108905
PubMed ID:
1586712
Type:
Article
Language:
en
ISSN:
0006-4971
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorLord, Brian Ien
dc.contributor.authorDexter, T Michaelen
dc.contributor.authorClements, J Men
dc.contributor.authorHunter, M Aen
dc.contributor.authorGearing, A Jen
dc.date.accessioned2010-08-03T10:36:44Z-
dc.date.available2010-08-03T10:36:44Z-
dc.date.issued1992-05-15-
dc.identifier.citationMacrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo. 1992, 79 (10):2605-9 Blooden
dc.identifier.issn0006-4971-
dc.identifier.pmid1586712-
dc.identifier.urihttp://hdl.handle.net/10541/108905-
dc.description.abstractMacrophage inflammatory protein-1 alpha (MIP-1 alpha) has been assessed for its potential in vivo to protect hematopoietic progenitor cells from the cytotoxic effects of a cycle-specific drug--in this case hydroxyurea (HU). Two doses of HU, 7 hours apart, were administered to mice to induce spleen colony-forming unit (CFU-S) cycling and then to kill them during DNA-synthesis. MIP-1 alpha, in a variety of dose and time combinations, was injected before the second dose of HU in an attempt to prevent recruitment or maintain CFU-S quiescence, and thus protect them from the second dose of HU. Without MIP-1 alpha, recovery of the CFU-S population was complete in 7 days. In a dose-dependent manner, MIP-1 alpha either reduced the initial kill and accelerated recovery, or completely protected the CFU-S population. We conclude that MIP-1 alpha does protect multipotent progenitor cells in vivo and that these observations provide a base from which to build practical clinical applications.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cells-
dc.subject.meshAnimals-
dc.subject.meshBone Marrow-
dc.subject.meshCells, Cultured-
dc.subject.meshChemokine CCL4-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCytokines-
dc.subject.meshFemale-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHydroxyurea-
dc.subject.meshKinetics-
dc.subject.meshMacrophage Inflammatory Proteins-
dc.subject.meshMice-
dc.subject.meshMice, Inbred Strains-
dc.subject.meshMonokines-
dc.subject.meshRecombinant Proteins-
dc.subject.meshTime Factors-
dc.titleMacrophage-inflammatory protein protects multipotent hematopoietic cells from the cytotoxic effects of hydroxyurea in vivo.en
dc.typeArticleen
dc.contributor.departmentCRC Department of Experimental Haematology, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Manchester, UK.en
dc.identifier.journalBlooden
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