Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108896
Title:
Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.
Authors:
West, Catharine M L; Moore, James V
Abstract:
The influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.
Affiliation:
Department of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.
Citation:
Mechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. 1992, 55 (3):425-30 Photochem. Photobiol.
Journal:
Photochemistry and Photobiology
Issue Date:
Mar-1992
URI:
http://hdl.handle.net/10541/108896
DOI:
10.1111/j.1751-1097.1992.tb04257.x
PubMed ID:
1532855
Type:
Article
Language:
en
ISSN:
0031-8655
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorWest, Catharine M Len
dc.contributor.authorMoore, James Ven
dc.date.accessioned2010-08-03T08:32:37Z-
dc.date.available2010-08-03T08:32:37Z-
dc.date.issued1992-03-
dc.identifier.citationMechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study. 1992, 55 (3):425-30 Photochem. Photobiol.en
dc.identifier.issn0031-8655-
dc.identifier.pmid1532855-
dc.identifier.doi10.1111/j.1751-1097.1992.tb04257.x-
dc.identifier.urihttp://hdl.handle.net/10541/108896-
dc.description.abstractThe influence of cell heterogeneity on response to photodynamic treatment (PDT) has been investigated using the human colon adenocarcinoma line WiDr, grown as spheroids and exposed to hematoporphyrin derivative. The spheroids show a marked spheroid size-dependent resistance to PDT. Using a flow cytometer, cell sub-populations have been separated, on the basis of drug fluorescence, from single cell suspensions prepared from 500 microm diameter spheroids. Cells low in fluorescence have been shown to be resistant to PDT, have a smaller median cell volume, and be enhanced in G1-type cells. These cells also show reduced low density lipoprotein uptake. The results suggest that spheroid size-dependent resistance to PDT is related to a decreasing growth fraction with increasing spheroid size. Heterogeneity of drug uptake could be a potential limitation to clinical PDT.en
dc.language.isoenen
dc.subjectHaematoporphyrinsen
dc.subjectHaematoporphyrin Derivativeen
dc.subjectCultured Tumour Cellsen
dc.subject.meshAdenocarcinoma-
dc.subject.meshAntineoplastic Agents-
dc.subject.meshBiological Transport, Active-
dc.subject.meshColonic Neoplasms-
dc.subject.meshDrug Resistance-
dc.subject.meshFlow Cytometry-
dc.subject.meshHematoporphyrin Derivative-
dc.subject.meshHematoporphyrins-
dc.subject.meshHumans-
dc.subject.meshPhotochemotherapy-
dc.subject.meshRadiation-Sensitizing Agents-
dc.subject.meshTumor Cells, Cultured-
dc.titleMechanisms behind the resistance of spheroids to photodynamic treatment: a flow cytometry study.en
dc.typeArticleen
dc.contributor.departmentDepartment of Experimental Radiation Oncology, Paterson Institute for Cancer Research, Christie Hospital, Manchester, U.K.en
dc.identifier.journalPhotochemistry and Photobiologyen
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