2.50
Hdl Handle:
http://hdl.handle.net/10541/108886
Title:
Growth of human umbilical-cord blood in longterm haemopoietic cultures.
Authors:
Hows, Jill M; Bradley, B A; Marsh, Judith C W; Luft, Thomas; Coutinho, Lucia H; Testa, Nydia G; Dexter, T Michael
Abstract:
Cryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered.
Affiliation:
Kay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.
Citation:
Growth of human umbilical-cord blood in longterm haemopoietic cultures. 1992, 340 (8811):73-6 Lancet
Journal:
Lancet
Issue Date:
11-Jul-1992
URI:
http://hdl.handle.net/10541/108886
DOI:
10.1016/0140-6736(92)90396-K
PubMed ID:
1352013
Type:
Article
Language:
en
ISSN:
0140-6736
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorHows, Jill Men
dc.contributor.authorBradley, B Aen
dc.contributor.authorMarsh, Judith C Wen
dc.contributor.authorLuft, Thomasen
dc.contributor.authorCoutinho, Lucia Hen
dc.contributor.authorTesta, Nydia Gen
dc.contributor.authorDexter, T Michaelen
dc.date.accessioned2010-08-03T08:55:33Z-
dc.date.available2010-08-03T08:55:33Z-
dc.date.issued1992-07-11-
dc.identifier.citationGrowth of human umbilical-cord blood in longterm haemopoietic cultures. 1992, 340 (8811):73-6 Lanceten
dc.identifier.issn0140-6736-
dc.identifier.pmid1352013-
dc.identifier.doi10.1016/0140-6736(92)90396-K-
dc.identifier.urihttp://hdl.handle.net/10541/108886-
dc.description.abstractCryopreserved human umbilical-cord (HUC) blood is an alternative to bone marrow as a source of haemopoietic "stem" cells for HLA-identical transplantation of children with leukaemia or Fanconi's anaemia. We have studied the in-vitro growth potential of HUC blood in clonogenic assays and in longterm haemopoietic cultures. Clonogenic assays showed that HUC blood produced as many haemopoietic-cell colonies as normal adult bone marrow and a higher proportion of primitive-cell colonies. In longterm culture on preformed irradiated marrow stroma, both progenitor-cell production and lifespan of cultures were significantly greater in HUC blood than in normal bone marrow (p = 0.0007). Our findings indicate that the quality and quantity of HUC-blood-derived haemopoietic "stem" cells are better than those of normal bone marrow. Therefore, single HUC-blood donations are probably sufficient for adults requiring transplantation for leukaemia and other haemopoietic disorders. Banking of HLA-typed HUC blood to facilitate transplantation of patients who lack a family donor should be considered.en
dc.language.isoenen
dc.subjectHaematopoietic Stem Cell Transplantationen
dc.subjectHaematopoietic Stem Cellsen
dc.subject.meshBlood Transfusion-
dc.subject.meshBone Marrow-
dc.subject.meshCell Division-
dc.subject.meshCell Survival-
dc.subject.meshCells, Cultured-
dc.subject.meshColony-Forming Units Assay-
dc.subject.meshCryopreservation-
dc.subject.meshErythroid Precursor Cells-
dc.subject.meshEvaluation Studies as Topic-
dc.subject.meshFetal Blood-
dc.subject.meshGranulocyte-Macrophage Colony-Stimulating Factor-
dc.subject.meshHematopoietic Stem Cell Transplantation-
dc.subject.meshHematopoietic Stem Cells-
dc.subject.meshHumans-
dc.subject.meshTime Factors-
dc.titleGrowth of human umbilical-cord blood in longterm haemopoietic cultures.en
dc.typeArticleen
dc.contributor.departmentKay Kendall Laboratory, Paterson Institute, Cancer Research Campaign, Manchester, UK.en
dc.identifier.journalLanceten

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