Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108879
Title:
Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.
Authors:
Baker, S M; Margison, Geoffrey P; Strike, P
Abstract:
We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.
Affiliation:
Department of Genetics and Microbiology, University of Liverpool, UK.
Citation:
Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. 1992, 20 (4):645-51 Nucleic Acids Res.
Journal:
Nucleic Acids Research
Issue Date:
25-Feb-1992
URI:
http://hdl.handle.net/10541/108879
DOI:
10.1093/nar/20.4.645
PubMed ID:
1542560
Type:
Article
Language:
en
ISSN:
0305-1048
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBaker, S Men
dc.contributor.authorMargison, Geoffrey Pen
dc.contributor.authorStrike, Pen
dc.date.accessioned2010-08-03T08:35:47Z-
dc.date.available2010-08-03T08:35:47Z-
dc.date.issued1992-02-25-
dc.identifier.citationInducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans. 1992, 20 (4):645-51 Nucleic Acids Res.en
dc.identifier.issn0305-1048-
dc.identifier.pmid1542560-
dc.identifier.doi10.1093/nar/20.4.645-
dc.identifier.urihttp://hdl.handle.net/10541/108879-
dc.description.abstractWe have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes a substantial induction of DNA alkyltransferase activity, with the specific activity in treated cells increasing up to one hundred-fold. Fluorography reveals the two main inducible species as proteins of 18.5 kDa and 21 kDa, both of which have activity primarily against O6-methylguanine (O6-MeG) lesions. In addition, two other alkyltransferase proteins can also be detected. One, of MW 16 kDa, is expressed in non-treated cells, but is not induced to the same extent as the 18.5 and 21 kDa proteins. The other, a protein of 19.5 kDa, is highly inducible and can only be detected in treated cells. Unlike the other three proteins, it acts primarily against methyl-phosphotriester (Me-PT) lesions. This is the first instance in which an MePT alkyltransferase has been detected in a eukaryotic organism and, coupled with the high level of induction of the O6-MeG alkyltransferase enzymes, this indicates that a control system similar to the bacterial adaptive response may be present in filamentous fungi.en
dc.language.isoenen
dc.subject.meshAlkyl and Aryl Transferases-
dc.subject.meshAspergillus nidulans-
dc.subject.meshDNA Repair-
dc.subject.meshDNA, Fungal-
dc.subject.meshElectrophoresis-
dc.subject.meshEnzyme Induction-
dc.subject.meshMethylnitronitrosoguanidine-
dc.subject.meshMethyltransferases-
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase-
dc.subject.meshTransferases-
dc.titleInducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.en
dc.typeArticleen
dc.contributor.departmentDepartment of Genetics and Microbiology, University of Liverpool, UK.en
dc.identifier.journalNucleic Acids Researchen
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