Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts.

2.50
Hdl Handle:
http://hdl.handle.net/10541/108878
Title:
Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts.
Authors:
Strickland, P T; Nikaido, O; Matsunaga, T; Boyle, John M
Abstract:
Monoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol. 34, 595-601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent light. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6-4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings.
Affiliation:
Johns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205.
Citation:
Further characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts. 1992, 55 (5):723-7 Photochem. Photobiol.
Journal:
Photochemistry and Photobiology
Issue Date:
May-1992
URI:
http://hdl.handle.net/10541/108878
DOI:
10.1111/j.1751-1097.1992.tb08516.x
PubMed ID:
1528986
Type:
Article
Language:
en
ISSN:
0031-8655
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorStrickland, P Ten
dc.contributor.authorNikaido, Oen
dc.contributor.authorMatsunaga, Ten
dc.contributor.authorBoyle, John Men
dc.date.accessioned2010-08-03T08:33:51Z-
dc.date.available2010-08-03T08:33:51Z-
dc.date.issued1992-05-
dc.identifier.citationFurther characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts. 1992, 55 (5):723-7 Photochem. Photobiol.en
dc.identifier.issn0031-8655-
dc.identifier.pmid1528986-
dc.identifier.doi10.1111/j.1751-1097.1992.tb08516.x-
dc.identifier.urihttp://hdl.handle.net/10541/108878-
dc.description.abstractMonoclonal antibody aUVssDNA-1 is produced by hybridoma cell line 25JF.C3B6 originally selected from cell fusions using spleen cells from mice immunized with UV-irradiated polydeoxynucleotides (Strickland and Boyle, Photochem. Photobiol. 34, 595-601, 1981). Original and subsequent studies of the binding characteristics of aUVssDNA-1 indicated that it was specific for cyclobuta-dithymidine photoproducts. Those investigations examined action spectrum, short-wavelength photo-reversal, nucleotide sequence effects, and photoreactivation using E. coli photolyase and incandescent light. However, the more recent studies reported here examined acetophenone-UV-B photosensitization, UV-B photoisomerization, and photoreactivation using cloned E. coli photolyase and filtered incandescent light. The results indicate that aUVssDNA-1 recognizes photoproducts with characteristics of (6-4)-dipyrimidines. Thus, previous studies in which relatively rapid repair of cyclobuta-dithymidine photoproducts was inferred using this antibody, require re-interpretation in light of these new findings.en
dc.language.isoenen
dc.subject.meshAnimals-
dc.subject.meshAntibodies, Monoclonal-
dc.subject.meshAntibody Specificity-
dc.subject.meshDNA, Single-Stranded-
dc.subject.meshPhotochemistry-
dc.subject.meshPyrimidine Dimers-
dc.subject.meshUltraviolet Rays-
dc.titleFurther characterization of monoclonal antibody indicates specificity for (6-4)-dipyrimidine photoproducts.en
dc.typeArticleen
dc.contributor.departmentJohns Hopkins School of Hygiene and Public Health, Baltimore, MD 21205.en
dc.identifier.journalPhotochemistry and Photobiologyen
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