2.50
Hdl Handle:
http://hdl.handle.net/10541/108877
Title:
K-ras gene mutations in adenomas and carcinomas of the colon.
Authors:
Boughdady, I S; Kinsella, Anne R; Haboubi, N Y; Schofield, Philip F
Abstract:
DNA extracted from 29 colorectal carcinomas and 40 sporadic adenomas was amplified by the polymerase chain reaction (PCR) and analysed for the presence of K-ras gene mutations at codon 12 using a panel of synthetic oligonucleotide probes specific for normal and mutated sequences. The presence of mutations was correlated with various histopathological and clinical data. Ten carcinomas (34.5%) and 14 sporadic adenomas (35%) showed K-ras mutations at codon 12. In the carcinoma group, no apparent correlation was found between the presence of mutant oncogenes and the degree of histological differentiation, Dukes' staging or the development of distant metastasis. In the adenoma group, the frequency of mutations increased with the size of the adenoma and the severity of the dysplastic changes. This study confirms that ras gene mutations are common and early events in colon carcinogenesis. They appear to give a selective growth advantage to those polyps with mutations which leads to their increase in size and thus possibly prepare the ground for malignant transformation.
Affiliation:
Paterson Institute for Cancer Research, Christie Hospital, Manchester, UK.
Citation:
K-ras gene mutations in adenomas and carcinomas of the colon. 1992, 1 (4):275-82 Surg Oncol
Journal:
Surgical Oncology
Issue Date:
Aug-1992
URI:
http://hdl.handle.net/10541/108877
DOI:
10.1016/0960-7404(92)90088-3
PubMed ID:
1341261
Type:
Article
Language:
en
ISSN:
0960-7404
Appears in Collections:
All Paterson Institute for Cancer Research

Full metadata record

DC FieldValue Language
dc.contributor.authorBoughdady, I Sen
dc.contributor.authorKinsella, Anne Ren
dc.contributor.authorHaboubi, N Yen
dc.contributor.authorSchofield, Philip Fen
dc.date.accessioned2010-08-03T08:25:29Z-
dc.date.available2010-08-03T08:25:29Z-
dc.date.issued1992-08-
dc.identifier.citationK-ras gene mutations in adenomas and carcinomas of the colon. 1992, 1 (4):275-82 Surg Oncolen
dc.identifier.issn0960-7404-
dc.identifier.pmid1341261-
dc.identifier.doi10.1016/0960-7404(92)90088-3-
dc.identifier.urihttp://hdl.handle.net/10541/108877-
dc.description.abstractDNA extracted from 29 colorectal carcinomas and 40 sporadic adenomas was amplified by the polymerase chain reaction (PCR) and analysed for the presence of K-ras gene mutations at codon 12 using a panel of synthetic oligonucleotide probes specific for normal and mutated sequences. The presence of mutations was correlated with various histopathological and clinical data. Ten carcinomas (34.5%) and 14 sporadic adenomas (35%) showed K-ras mutations at codon 12. In the carcinoma group, no apparent correlation was found between the presence of mutant oncogenes and the degree of histological differentiation, Dukes' staging or the development of distant metastasis. In the adenoma group, the frequency of mutations increased with the size of the adenoma and the severity of the dysplastic changes. This study confirms that ras gene mutations are common and early events in colon carcinogenesis. They appear to give a selective growth advantage to those polyps with mutations which leads to their increase in size and thus possibly prepare the ground for malignant transformation.en
dc.language.isoenen
dc.subjectColonic Canceren
dc.subject.meshAdenoma-
dc.subject.meshAdult-
dc.subject.meshAged-
dc.subject.meshBase Sequence-
dc.subject.meshCarcinoma-
dc.subject.meshCodon-
dc.subject.meshColonic Neoplasms-
dc.subject.meshColonic Polyps-
dc.subject.meshDNA-
dc.subject.meshFemale-
dc.subject.meshGenes, ras-
dc.subject.meshHumans-
dc.subject.meshImmunoblotting-
dc.subject.meshMale-
dc.subject.meshMiddle Aged-
dc.subject.meshMolecular Sequence Data-
dc.subject.meshMutation-
dc.subject.meshNucleic Acid Hybridization-
dc.subject.meshOligonucleotide Probes-
dc.subject.meshPolymerase Chain Reaction-
dc.titleK-ras gene mutations in adenomas and carcinomas of the colon.en
dc.typeArticleen
dc.contributor.departmentPaterson Institute for Cancer Research, Christie Hospital, Manchester, UK.en
dc.identifier.journalSurgical Oncologyen

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